The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study

Objectives. The aim of this in vitro study was to observe through scanning electron microscopy (SEM) the surface morphology of root samples treated with different mechanical instrumentation methods and the additional application of a nanocolloidal silver-based antiseptic solution. Material and meth...

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Main Authors: Andrada Soanca, Alexandra Roman, Andreea Ciurea, Iulia Cristina Micu, Andrei Picos, Sofia Iozon (Ene)
Format: Article
Language:English
Published: Amaltea Medical Publishing House 2023-03-01
Series:Romanian Journal of Stomatology
Subjects:
Online Access:https://rjs.com.ro/articles/2023.1/RJS_2023_1_Art-08.pdf
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author Andrada Soanca
Alexandra Roman
Andreea Ciurea
Iulia Cristina Micu
Andrei Picos
Sofia Iozon (Ene)
author_facet Andrada Soanca
Alexandra Roman
Andreea Ciurea
Iulia Cristina Micu
Andrei Picos
Sofia Iozon (Ene)
author_sort Andrada Soanca
collection DOAJ
description Objectives. The aim of this in vitro study was to observe through scanning electron microscopy (SEM) the surface morphology of root samples treated with different mechanical instrumentation methods and the additional application of a nanocolloidal silver-based antiseptic solution. Material and methods. Root samples were prepared from extracted molars and divided in four groups: group 1 of samples was instrumented with Gracey curette (7/8), group 2 of samples was instrumented with ultrasonic tip 1S, group 3 of samples was instrumented with ultrasonic tips 1S plus H4R/L, group 4 of samples was treated with ultrasonic tips 1S plus H4R/L and application of the silver-based antiseptic solution. All samples were then subjected to SEM examination. Outcomes. Group 1 of samples presented a smoother surface compared to the samples in the other groups. Group 2, 3 and 4 displayed superficial grooves parallel to the direction of action of the ultrasonic tip. In group 3 and 4, root planning with tip H4R/L did not ameliorate the smoothness of the surface. The smear layer was present, regardless the instrumentation method. In group 4, the additional application of the antiseptic solution did not influence the surface morphology or the amount of smear layer. Conclusions. Gracey curettes created a smoother radicular surface compared to ultrasonic tips. Smear layer was obvious on the radicular surfaces, no matter the instrumentation method. The application of the silver-based antiseptic solution had no additional impact on surface morphology.
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spelling doaj.art-53f355667b2a4e69944a2177bb7c56332023-09-14T14:09:09ZengAmaltea Medical Publishing HouseRomanian Journal of Stomatology1843-08052069-60782023-03-01681434910.37897/RJS.2023.1.8The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro studyAndrada Soanca0Alexandra Roman1Andreea Ciurea2Iulia Cristina Micu3Andrei Picos4Sofia Iozon (Ene)5Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Prevention in Dental Medicine, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Odontology, Endodontics and Oral Pathology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaObjectives. The aim of this in vitro study was to observe through scanning electron microscopy (SEM) the surface morphology of root samples treated with different mechanical instrumentation methods and the additional application of a nanocolloidal silver-based antiseptic solution. Material and methods. Root samples were prepared from extracted molars and divided in four groups: group 1 of samples was instrumented with Gracey curette (7/8), group 2 of samples was instrumented with ultrasonic tip 1S, group 3 of samples was instrumented with ultrasonic tips 1S plus H4R/L, group 4 of samples was treated with ultrasonic tips 1S plus H4R/L and application of the silver-based antiseptic solution. All samples were then subjected to SEM examination. Outcomes. Group 1 of samples presented a smoother surface compared to the samples in the other groups. Group 2, 3 and 4 displayed superficial grooves parallel to the direction of action of the ultrasonic tip. In group 3 and 4, root planning with tip H4R/L did not ameliorate the smoothness of the surface. The smear layer was present, regardless the instrumentation method. In group 4, the additional application of the antiseptic solution did not influence the surface morphology or the amount of smear layer. Conclusions. Gracey curettes created a smoother radicular surface compared to ultrasonic tips. Smear layer was obvious on the radicular surfaces, no matter the instrumentation method. The application of the silver-based antiseptic solution had no additional impact on surface morphology.https://rjs.com.ro/articles/2023.1/RJS_2023_1_Art-08.pdfsubgingival mechanical instrumentationroot surfaceantisepticsilver nanoparticlesscanning electron microscopy
spellingShingle Andrada Soanca
Alexandra Roman
Andreea Ciurea
Iulia Cristina Micu
Andrei Picos
Sofia Iozon (Ene)
The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
Romanian Journal of Stomatology
subgingival mechanical instrumentation
root surface
antiseptic
silver nanoparticles
scanning electron microscopy
title The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
title_full The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
title_fullStr The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
title_full_unstemmed The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
title_short The evaluation of root surface modifications after different subgingival mechanical instrumentation techniques – an in vitro study
title_sort evaluation of root surface modifications after different subgingival mechanical instrumentation techniques an in vitro study
topic subgingival mechanical instrumentation
root surface
antiseptic
silver nanoparticles
scanning electron microscopy
url https://rjs.com.ro/articles/2023.1/RJS_2023_1_Art-08.pdf
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