Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection
<i>Rhizoctonia solani</i> is a soil-borne fungus causing sheath blight disease in cereal crops including rice. Genetic resistance to sheath blight disease in cereal crops is not well understood in most of the host(s). Aside from this, a comparative study on the different hosts at the bio...
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MDPI AG
2022-10-01
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| author | Md. Shamim Divakar Sharma Deepa Bisht Rashmi Maurya Mayank Kaashyap Deepti Srivastava Anurag Mishra Deepak Kumar Mahesh Kumar Vijaya Naresh Juturu N. A. Khan Sameer Chaudhary Raja Hussain K. N. Singh |
| author_facet | Md. Shamim Divakar Sharma Deepa Bisht Rashmi Maurya Mayank Kaashyap Deepti Srivastava Anurag Mishra Deepak Kumar Mahesh Kumar Vijaya Naresh Juturu N. A. Khan Sameer Chaudhary Raja Hussain K. N. Singh |
| author_sort | Md. Shamim |
| collection | DOAJ |
| description | <i>Rhizoctonia solani</i> is a soil-borne fungus causing sheath blight disease in cereal crops including rice. Genetic resistance to sheath blight disease in cereal crops is not well understood in most of the host(s). Aside from this, a comparative study on the different hosts at the biochemical and proteomic level upon <i>R. solani</i> infection was not reported earlier. Here, we performed proteomic based analysis and studied defense pathways among cultivated rice (cv. Pusa Basmati-1), wild rice accession (<i>Oryza grandiglumis</i>), and barley (cv. NDB-1445) after inoculation with <i>R. solani</i>. Increased levels of phenol, peroxidase, and β-1, 3-glucanase were observed in infected tissue as compared to the control in all of the hosts. Wild rice accession <i>O. grandiglumis</i> showed a higher level of biochemical signals than barley cv. NDB 1445 and cultivated rice cv. Pusa Basmati-1. Using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS), differently expressed proteins were also studied in control and after inoculation with <i>R. solani</i>. Wild rice accession <i>O. grandiglumis</i> induced a cysteine protease inhibitor and zinc finger proteins, which have defense functions and resistance against fungal pathogens. On the other hand, barley cv. NDB-1445 and cultivated rice cv. Pusa Basmati-1 mainly induce energy metabolism-related proteins/signals after inoculation with <i>R. solani</i> in comparison to wild rice accession <i>O. grandiglumis</i>. The present comprehensive study of <i>R. solani</i> interaction using three hosts, namely, Pusa Basmati-1 (cultivated rice), <i>O. grandiglumis</i> (wild rice), and NDB-1445 (barley) would interpret wider possibilities in the dissection of the protein(s) induced during the infection process. These proteins may further be correlated to the gene(s) and other related molecular tools that will help for the marker-assisted breeding and/or gene editing for this distressing disease among the major cereal crops. |
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| spelling | doaj.art-53f760d4d06b4af3acd5dcd0164fdc392023-11-23T22:58:19ZengMDPI AGBioengineering2306-53542022-10-0191058910.3390/bioengineering9100589Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> InfectionMd. Shamim0Divakar Sharma1Deepa Bisht2Rashmi Maurya3Mayank Kaashyap4Deepti Srivastava5Anurag Mishra6Deepak Kumar7Mahesh Kumar8Vijaya Naresh Juturu9N. A. Khan10Sameer Chaudhary11Raja Hussain12K. N. Singh13Department of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaDepartment of Microbiology, Maulana Azad Medical College, Bahadur Shah Zafar Marg, New Delhi 110002, Delhi, IndiaDepartment of Biochemistry, ICMR-National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282001, Uttar Pradesh, IndiaSchool of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi 110067, Delhi, IndiaSchool of Life Science, RMIT University, Bundoora, Melbourne, VIC 3083, AustraliaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaDepartment of Molecular Biology and Genetic Engineering, Dr. Kalam Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Arrabari, Kishanganj 855107, Bihar, IndiaAgri Biotech Foundation, Formerly A P Netherlands Biotechnology, Programme, Rajendra Nagar, Hyderabad 500030, Telangana, IndiaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaRASA Life Science Informatics, Law College Road, Pune 411052, Maharashtra, IndiaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, IndiaDepartment of Plant Molecular Biology and Genetic Engineering, A.N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224229, Uttar Pradesh, India<i>Rhizoctonia solani</i> is a soil-borne fungus causing sheath blight disease in cereal crops including rice. Genetic resistance to sheath blight disease in cereal crops is not well understood in most of the host(s). Aside from this, a comparative study on the different hosts at the biochemical and proteomic level upon <i>R. solani</i> infection was not reported earlier. Here, we performed proteomic based analysis and studied defense pathways among cultivated rice (cv. Pusa Basmati-1), wild rice accession (<i>Oryza grandiglumis</i>), and barley (cv. NDB-1445) after inoculation with <i>R. solani</i>. Increased levels of phenol, peroxidase, and β-1, 3-glucanase were observed in infected tissue as compared to the control in all of the hosts. Wild rice accession <i>O. grandiglumis</i> showed a higher level of biochemical signals than barley cv. NDB 1445 and cultivated rice cv. Pusa Basmati-1. Using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS), differently expressed proteins were also studied in control and after inoculation with <i>R. solani</i>. Wild rice accession <i>O. grandiglumis</i> induced a cysteine protease inhibitor and zinc finger proteins, which have defense functions and resistance against fungal pathogens. On the other hand, barley cv. NDB-1445 and cultivated rice cv. Pusa Basmati-1 mainly induce energy metabolism-related proteins/signals after inoculation with <i>R. solani</i> in comparison to wild rice accession <i>O. grandiglumis</i>. The present comprehensive study of <i>R. solani</i> interaction using three hosts, namely, Pusa Basmati-1 (cultivated rice), <i>O. grandiglumis</i> (wild rice), and NDB-1445 (barley) would interpret wider possibilities in the dissection of the protein(s) induced during the infection process. These proteins may further be correlated to the gene(s) and other related molecular tools that will help for the marker-assisted breeding and/or gene editing for this distressing disease among the major cereal crops.https://www.mdpi.com/2306-5354/9/10/589<i>Rhizoctonia solani</i>pathogenesiscereal cropsproteomics |
| spellingShingle | Md. Shamim Divakar Sharma Deepa Bisht Rashmi Maurya Mayank Kaashyap Deepti Srivastava Anurag Mishra Deepak Kumar Mahesh Kumar Vijaya Naresh Juturu N. A. Khan Sameer Chaudhary Raja Hussain K. N. Singh Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection Bioengineering <i>Rhizoctonia solani</i> pathogenesis cereal crops proteomics |
| title | Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection |
| title_full | Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection |
| title_fullStr | Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection |
| title_full_unstemmed | Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection |
| title_short | Proteo-Molecular Investigation of Cultivated Rice, Wild Rice, and Barley Provides Clues of Defense Responses against <i>Rhizoctonia solani</i> Infection |
| title_sort | proteo molecular investigation of cultivated rice wild rice and barley provides clues of defense responses against i rhizoctonia solani i infection |
| topic | <i>Rhizoctonia solani</i> pathogenesis cereal crops proteomics |
| url | https://www.mdpi.com/2306-5354/9/10/589 |
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