Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats
Purpose Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill run...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Continence Society
2018-10-01
|
| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-1836226-113.pdf |
| _version_ | 1819027522093842432 |
|---|---|
| author | Jae-Min Lee Tae-Woon Kim Sang-Seo Park Jin-Hee Han Mal-Soon Shin Baek-Vin Lim Sang-Hoon Kim Seung-Soo Baek Young Sam Cho Khae Hawn Kim |
| author_facet | Jae-Min Lee Tae-Woon Kim Sang-Seo Park Jin-Hee Han Mal-Soon Shin Baek-Vin Lim Sang-Hoon Kim Seung-Soo Baek Young Sam Cho Khae Hawn Kim |
| author_sort | Jae-Min Lee |
| collection | DOAJ |
| description | Purpose Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill running improves motor ability using rotenone-caused PD rats. The effect of treadmill running on PD was also assessed in relation with apoptosis of cerebellar Purkinje cells. Methods Treadmill running was applied to the rats in the exercise groups for 30 minutes once a day for 4 weeks, starting 4 weeks after birth. We used rota-rod test for the determination of motor coordination and balance. In this experiment, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for calbindin, glial fibrillary acidic protein (GFAP), Iba-1, and western blot analysis for Bax and Bcl-2 were performed. Results Treadmill running enhanced motor balance and coordination by preventing the loss of Purkinje cells in the cerebellar vermis. Treadmill running suppressed PD-induced expression of GFAP-positive reactive astrocytes and Iba-1-positive microglia, showing that treadmill running suppressed reactive astrogliosis and microglia activation. Treadmill running suppressed TUNEL-positive cell number and Bax expression and enhanced Bcl-2 expression, demonstrating that treadmill running inhibited the progress of apoptosis in the cerebellum of rotenone-induced PD rats. Conclusions Treadmill running improved motor ability of the rotenone-induced PD rats by inhibiting apoptosis in the cerebellum. Apoptosis suppressing effect of treadmill running on rotenone-induced PD was achieved via suppression of reactive astrocyte and inhibition of microglial activation. |
| first_indexed | 2024-12-21T05:43:48Z |
| format | Article |
| id | doaj.art-53f8c0710bfc42b88167da10ee04884f |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-12-21T05:43:48Z |
| publishDate | 2018-10-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-53f8c0710bfc42b88167da10ee04884f2022-12-21T19:14:12ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312018-10-0122Suppl 3S14715510.5213/inj.1836226.113756Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease RatsJae-Min Lee0Tae-Woon Kim1Sang-Seo Park2Jin-Hee Han3Mal-Soon Shin4Baek-Vin Lim5Sang-Hoon Kim6Seung-Soo Baek7Young Sam Cho8Khae Hawn Kim9 Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea School of Global Sport Studies, Korea University, Sejong, Korea Division of Leisure & Sports Science, Department of Exercise Prescription, Dongseo University, Busan, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Sport & Health Science, College of Natural Science, Sangmyung University, Seoul, Korea Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea Department of Urology, Gil Medical Center, Gachon University School of Medicine, Incheon, KoreaPurpose Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill running improves motor ability using rotenone-caused PD rats. The effect of treadmill running on PD was also assessed in relation with apoptosis of cerebellar Purkinje cells. Methods Treadmill running was applied to the rats in the exercise groups for 30 minutes once a day for 4 weeks, starting 4 weeks after birth. We used rota-rod test for the determination of motor coordination and balance. In this experiment, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for calbindin, glial fibrillary acidic protein (GFAP), Iba-1, and western blot analysis for Bax and Bcl-2 were performed. Results Treadmill running enhanced motor balance and coordination by preventing the loss of Purkinje cells in the cerebellar vermis. Treadmill running suppressed PD-induced expression of GFAP-positive reactive astrocytes and Iba-1-positive microglia, showing that treadmill running suppressed reactive astrogliosis and microglia activation. Treadmill running suppressed TUNEL-positive cell number and Bax expression and enhanced Bcl-2 expression, demonstrating that treadmill running inhibited the progress of apoptosis in the cerebellum of rotenone-induced PD rats. Conclusions Treadmill running improved motor ability of the rotenone-induced PD rats by inhibiting apoptosis in the cerebellum. Apoptosis suppressing effect of treadmill running on rotenone-induced PD was achieved via suppression of reactive astrocyte and inhibition of microglial activation.http://www.einj.org/upload/pdf/inj-1836226-113.pdfParkinson diseaseRotenone, CerebellumMotor coordinationTreadmill exerciseApoptosis |
| spellingShingle | Jae-Min Lee Tae-Woon Kim Sang-Seo Park Jin-Hee Han Mal-Soon Shin Baek-Vin Lim Sang-Hoon Kim Seung-Soo Baek Young Sam Cho Khae Hawn Kim Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats International Neurourology Journal Parkinson disease Rotenone, Cerebellum Motor coordination Treadmill exercise Apoptosis |
| title | Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats |
| title_full | Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats |
| title_fullStr | Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats |
| title_full_unstemmed | Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats |
| title_short | Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats |
| title_sort | treadmill exercise improves motor function by suppressing purkinje cell loss in parkinson disease rats |
| topic | Parkinson disease Rotenone, Cerebellum Motor coordination Treadmill exercise Apoptosis |
| url | http://www.einj.org/upload/pdf/inj-1836226-113.pdf |
| work_keys_str_mv | AT jaeminlee treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT taewoonkim treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT sangseopark treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT jinheehan treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT malsoonshin treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT baekvinlim treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT sanghoonkim treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT seungsoobaek treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT youngsamcho treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats AT khaehawnkim treadmillexerciseimprovesmotorfunctionbysuppressingpurkinjecelllossinparkinsondiseaserats |