Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina
Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dai...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
|
| Series: | Revista Argentina de Microbiología |
| Subjects: | |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000200009&lng=en&tlng=en |
| _version_ | 1818482695305428992 |
|---|---|
| author | Pablo J Tamiozzo Abel A Estanguet Julia Maito Liliana Tirante Martin Pol José A Giraudo |
| author_facet | Pablo J Tamiozzo Abel A Estanguet Julia Maito Liliana Tirante Martin Pol José A Giraudo |
| author_sort | Pablo J Tamiozzo |
| collection | DOAJ |
| description | Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (16S-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively. |
| first_indexed | 2024-12-10T11:50:42Z |
| format | Article |
| id | doaj.art-53f8eca0576f4c8395e629f2a22a3566 |
| institution | Directory Open Access Journal |
| issn | 1851-7617 |
| language | English |
| last_indexed | 2024-12-10T11:50:42Z |
| publisher | Elsevier España |
| record_format | Article |
| series | Revista Argentina de Microbiología |
| spelling | doaj.art-53f8eca0576f4c8395e629f2a22a35662022-12-22T01:49:56ZengElsevier EspañaRevista Argentina de Microbiología1851-7617462119121S0325-75412014000200009Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from ArgentinaPablo J Tamiozzo0Abel A Estanguet1Julia Maito2Liliana Tirante3Martin Pol4José A Giraudo5Universidad Nacional de Río CuartoUniversidad Nacional de Río CuartoSociedad Responsabilidad LimitadaSociedad Responsabilidad LimitadaSociedad Responsabilidad LimitadaUniversidad Nacional de Río CuartoDifferent species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (16S-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000200009&lng=en&tlng=enGanado lecheroMycoplasma bovisMycoplasma canadenseMycoplasma californicumITS16S-23S ARNr |
| spellingShingle | Pablo J Tamiozzo Abel A Estanguet Julia Maito Liliana Tirante Martin Pol José A Giraudo Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina Revista Argentina de Microbiología Ganado lechero Mycoplasma bovis Mycoplasma canadense Mycoplasma californicum ITS 16S-23S ARNr |
| title | Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina |
| title_full | Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina |
| title_fullStr | Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina |
| title_full_unstemmed | Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina |
| title_short | Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina |
| title_sort | detection of mycoplasma canadense and mycoplasma californicum in dairy cattle from argentina |
| topic | Ganado lechero Mycoplasma bovis Mycoplasma canadense Mycoplasma californicum ITS 16S-23S ARNr |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000200009&lng=en&tlng=en |
| work_keys_str_mv | AT pablojtamiozzo detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina AT abelaestanguet detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina AT juliamaito detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina AT lilianatirante detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina AT martinpol detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina AT joseagiraudo detectionofmycoplasmacanadenseandmycoplasmacalifornicumindairycattlefromargentina |