Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
Cadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresse...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.843725/full |
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| author | Pei-Long Wang Pei-Long Wang Xiao-Jin Lei Yuan-Yuan Wang Bai-chao Liu Dan-ni Wang Zhong-Yuan Liu Cai-Qiu Gao |
| author_facet | Pei-Long Wang Pei-Long Wang Xiao-Jin Lei Yuan-Yuan Wang Bai-chao Liu Dan-ni Wang Zhong-Yuan Liu Cai-Qiu Gao |
| author_sort | Pei-Long Wang |
| collection | DOAJ |
| description | Cadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresses. However, no systematic study has investigated the molecular mechanism of Cd tolerance in T. hispida. In the study, RNA-seq technique was applied to analyze the transcriptomic changes in T. hispida treated with 150 μmol L–1 CdCl2 for 24, 48, and 72 h compared with control. In total, 72,764 unigenes exhibited similar sequences in the Non-redundant nucleic acid database (NR database), while 36.3% of all these unigenes may be new transcripts. In addition, 6,778, 8,282, and 8,601 DEGs were detected at 24, 48, and 72 h, respectively. Functional annotation analysis indicated that many genes may be involved in Cd stress response, including ion bonding, signal transduction, stress sensing, hormone responses and ROS metabolism. A ThUGT gene from the abscisic acid (ABA) signaling pathway can enhance Cd resistance ability of T. hispida by regulating the production of ROS under Cd stress and inhibit absorption of Cd. The new transcriptome resources and data that we present in this study for T. hispida may facilitate investigation of molecular mechanisms governing Cd resistance. |
| first_indexed | 2024-12-12T21:31:20Z |
| format | Article |
| id | doaj.art-53fe73e9fd4549a3842607e3f8c68ee1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T21:31:20Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-53fe73e9fd4549a3842607e3f8c68ee12022-12-22T00:11:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.843725843725Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid NetworkPei-Long Wang0Pei-Long Wang1Xiao-Jin Lei2Yuan-Yuan Wang3Bai-chao Liu4Dan-ni Wang5Zhong-Yuan Liu6Cai-Qiu Gao7State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaZhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaCadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresses. However, no systematic study has investigated the molecular mechanism of Cd tolerance in T. hispida. In the study, RNA-seq technique was applied to analyze the transcriptomic changes in T. hispida treated with 150 μmol L–1 CdCl2 for 24, 48, and 72 h compared with control. In total, 72,764 unigenes exhibited similar sequences in the Non-redundant nucleic acid database (NR database), while 36.3% of all these unigenes may be new transcripts. In addition, 6,778, 8,282, and 8,601 DEGs were detected at 24, 48, and 72 h, respectively. Functional annotation analysis indicated that many genes may be involved in Cd stress response, including ion bonding, signal transduction, stress sensing, hormone responses and ROS metabolism. A ThUGT gene from the abscisic acid (ABA) signaling pathway can enhance Cd resistance ability of T. hispida by regulating the production of ROS under Cd stress and inhibit absorption of Cd. The new transcriptome resources and data that we present in this study for T. hispida may facilitate investigation of molecular mechanisms governing Cd resistance.https://www.frontiersin.org/articles/10.3389/fpls.2022.843725/fullTamarix hispida Willdcadmium stresstranscriptomic analysisdifferentially expressed genesH2O2ABA |
| spellingShingle | Pei-Long Wang Pei-Long Wang Xiao-Jin Lei Yuan-Yuan Wang Bai-chao Liu Dan-ni Wang Zhong-Yuan Liu Cai-Qiu Gao Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network Frontiers in Plant Science Tamarix hispida Willd cadmium stress transcriptomic analysis differentially expressed genes H2O2 ABA |
| title | Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network |
| title_full | Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network |
| title_fullStr | Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network |
| title_full_unstemmed | Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network |
| title_short | Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network |
| title_sort | transcriptomic analysis of cadmium stressed tamarix hispida revealed novel transcripts and the importance of abscisic acid network |
| topic | Tamarix hispida Willd cadmium stress transcriptomic analysis differentially expressed genes H2O2 ABA |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.843725/full |
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