Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting
Solid or liquid waste containing a high concentration of nicotine can pollute sediment in rivers and lakes, and may destroy the ecological balance if it is directly discharged into the environment without any treatment. In this study, the polymerase chain reaction (PCR) and denaturing gradient gel e...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Water Science and Engineering |
| Subjects: | |
| Online Access: | http://www.waterjournal.cn:8080/water/EN/abstract/abstract356.shtml |
| _version_ | 1823999370960306176 |
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| author | Ai-dong Ruan Chen-xiao Liu |
| author_facet | Ai-dong Ruan Chen-xiao Liu |
| author_sort | Ai-dong Ruan |
| collection | DOAJ |
| description | Solid or liquid waste containing a high concentration of nicotine can pollute sediment in rivers and lakes, and may destroy the ecological balance if it is directly discharged into the environment without any treatment. In this study, the polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) method was used to analyze the variation of the microbial community structure in the control and nicotine-contaminated sediment samples with nicotine concentration and time of exposure. The results demonstrated that the growth of some bacterial species in the nicotine-contaminated sediment samples was inhibited during the exposure. Some bacteria decreased in species diversity and in quantity with the increase of nicotine concentration or time of exposure, while other bacteria were enriched under the effect of nicotine, and their DGGE bands changed from undertones to deep colors. The microbial community structure, however, showed a wide variation in the nicotine-contaminated sediment samples, especially in the sediment samples treated with high-concentration nicotine. The Jaccard index was only 35.1% between the initial sediment sample and the sediment sample with a nicotine concentration of 0.030 μg/g after 28 d of exposure. Diversity indices showed that the contaminated groups had a similar trend over time. The diversity indices of contaminated groups all decreased in the first 7 d after exposure, then increased until day 42. It has been found that nicotine decreased the diversity of the microbial community in the sediment. |
| first_indexed | 2024-12-18T17:43:29Z |
| format | Article |
| id | doaj.art-5400a410d2664c8a8e2030ad8645cdef |
| institution | Directory Open Access Journal |
| issn | 1674-2370 2405-8106 |
| language | English |
| last_indexed | 2024-12-18T17:43:29Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Water Science and Engineering |
| spelling | doaj.art-5400a410d2664c8a8e2030ad8645cdef2022-12-21T20:59:05ZengElsevierWater Science and Engineering1674-23702405-81062015-10-0184309314Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprintingAi-dong Ruan0Chen-xiao Liu1State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, PR ChinaState Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, PR ChinaSolid or liquid waste containing a high concentration of nicotine can pollute sediment in rivers and lakes, and may destroy the ecological balance if it is directly discharged into the environment without any treatment. In this study, the polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) method was used to analyze the variation of the microbial community structure in the control and nicotine-contaminated sediment samples with nicotine concentration and time of exposure. The results demonstrated that the growth of some bacterial species in the nicotine-contaminated sediment samples was inhibited during the exposure. Some bacteria decreased in species diversity and in quantity with the increase of nicotine concentration or time of exposure, while other bacteria were enriched under the effect of nicotine, and their DGGE bands changed from undertones to deep colors. The microbial community structure, however, showed a wide variation in the nicotine-contaminated sediment samples, especially in the sediment samples treated with high-concentration nicotine. The Jaccard index was only 35.1% between the initial sediment sample and the sediment sample with a nicotine concentration of 0.030 μg/g after 28 d of exposure. Diversity indices showed that the contaminated groups had a similar trend over time. The diversity indices of contaminated groups all decreased in the first 7 d after exposure, then increased until day 42. It has been found that nicotine decreased the diversity of the microbial community in the sediment.http://www.waterjournal.cn:8080/water/EN/abstract/abstract356.shtmlnicotinesedimentPCR-DGGEmicrobial community structurediversity indices |
| spellingShingle | Ai-dong Ruan Chen-xiao Liu Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting Water Science and Engineering nicotine sediment PCR-DGGE microbial community structure diversity indices |
| title | Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting |
| title_full | Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting |
| title_fullStr | Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting |
| title_full_unstemmed | Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting |
| title_short | Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting |
| title_sort | analysis of effect of nicotine on microbial community structure in sediment using pcr dgge fingerprinting |
| topic | nicotine sediment PCR-DGGE microbial community structure diversity indices |
| url | http://www.waterjournal.cn:8080/water/EN/abstract/abstract356.shtml |
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