CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis
Introduction: Circular RNAs (circRNAs) are important regulators in various cancers, especially hepatocellular carcinoma. However, the role of circ RNA PTPRM (circPTPRM) in the development of non-small-cell lung cancer (NSCLC) remains unclear. Methods: We collected 26 clinical specimens (correspondin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-05-01
|
| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338221090090 |
| _version_ | 1818038401694171136 |
|---|---|
| author | Zeyong Jiang MD Jian Zhao MD Hanlin Zou BM Kaican Cai MD |
| author_facet | Zeyong Jiang MD Jian Zhao MD Hanlin Zou BM Kaican Cai MD |
| author_sort | Zeyong Jiang MD |
| collection | DOAJ |
| description | Introduction: Circular RNAs (circRNAs) are important regulators in various cancers, especially hepatocellular carcinoma. However, the role of circ RNA PTPRM (circPTPRM) in the development of non-small-cell lung cancer (NSCLC) remains unclear. Methods: We collected 26 clinical specimens (corresponding to 26 normal lung tissues) of lung adenocarcinoma and the expression of mir-139-5p and circPTPRM were first detected. Cell proliferation was detected by EdU method, invasion/migration ability of cells was evaluated by transwell method. And the correlation between circPTPRM and mir-139-5p was detected by luciferase reporter gene and RNA pull-down assay. Finally, we verified our hypothesis with BALB/c nude mice. Results: Through bioinformatics software, we found that circPTPRM was negatively correlated with mir-139-5p, and then we used human adenocarcinoma tissue samples to further verify their relationship and get the same result. EdU method, transwell method, and luciferase assay, RNA pull-down assay were applied, and the results show that the knockdown of circPTPRM inhibit proliferation, migration, and invasion of cells can be reversed by mir-139-5p inhibitor. Next, we used Starbase v2.0 to identify the target site of miR-139-5p and focused on SET domain containing 5 (SETD5). We derive the hypothesis by verifying the relationship between miR-139-5p and SETD5 that circPTPRM may interact with miR-139-5p/SETD5 axis. At last, we evaluated the effects of circPTPRM, SETD5, and miR-139-5p on tumor growth in vivo using BALB/c nude mice to prove the hypothesis. Conclusion: We thus conclude that circPTPRM promotes the progression of NSCLC by regulating the miR-139-5p/SETD5 axis. |
| first_indexed | 2024-12-10T07:42:09Z |
| format | Article |
| id | doaj.art-540128ff4978493ca84663a38f3a0028 |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-12-10T07:42:09Z |
| publishDate | 2022-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-540128ff4978493ca84663a38f3a00282022-12-22T01:57:17ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382022-05-012110.1177/15330338221090090CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 AxisZeyong Jiang MD0Jian Zhao MD1Hanlin Zou BM2Kaican Cai MD3 Nanfang Hospital, , Guangzhou, Guangdong, China Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, China People’s Hospital of Chenghai, Shantou, China Nanfang Hospital, , Guangzhou, Guangdong, ChinaIntroduction: Circular RNAs (circRNAs) are important regulators in various cancers, especially hepatocellular carcinoma. However, the role of circ RNA PTPRM (circPTPRM) in the development of non-small-cell lung cancer (NSCLC) remains unclear. Methods: We collected 26 clinical specimens (corresponding to 26 normal lung tissues) of lung adenocarcinoma and the expression of mir-139-5p and circPTPRM were first detected. Cell proliferation was detected by EdU method, invasion/migration ability of cells was evaluated by transwell method. And the correlation between circPTPRM and mir-139-5p was detected by luciferase reporter gene and RNA pull-down assay. Finally, we verified our hypothesis with BALB/c nude mice. Results: Through bioinformatics software, we found that circPTPRM was negatively correlated with mir-139-5p, and then we used human adenocarcinoma tissue samples to further verify their relationship and get the same result. EdU method, transwell method, and luciferase assay, RNA pull-down assay were applied, and the results show that the knockdown of circPTPRM inhibit proliferation, migration, and invasion of cells can be reversed by mir-139-5p inhibitor. Next, we used Starbase v2.0 to identify the target site of miR-139-5p and focused on SET domain containing 5 (SETD5). We derive the hypothesis by verifying the relationship between miR-139-5p and SETD5 that circPTPRM may interact with miR-139-5p/SETD5 axis. At last, we evaluated the effects of circPTPRM, SETD5, and miR-139-5p on tumor growth in vivo using BALB/c nude mice to prove the hypothesis. Conclusion: We thus conclude that circPTPRM promotes the progression of NSCLC by regulating the miR-139-5p/SETD5 axis.https://doi.org/10.1177/15330338221090090 |
| spellingShingle | Zeyong Jiang MD Jian Zhao MD Hanlin Zou BM Kaican Cai MD CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis Technology in Cancer Research & Treatment |
| title | CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis |
| title_full | CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis |
| title_fullStr | CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis |
| title_full_unstemmed | CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis |
| title_short | CircRNA PTPRM Promotes Non-Small Cell Lung Cancer Progression by Modulating the miR-139-5p/SETD5 Axis |
| title_sort | circrna ptprm promotes non small cell lung cancer progression by modulating the mir 139 5p setd5 axis |
| url | https://doi.org/10.1177/15330338221090090 |
| work_keys_str_mv | AT zeyongjiangmd circrnaptprmpromotesnonsmallcelllungcancerprogressionbymodulatingthemir1395psetd5axis AT jianzhaomd circrnaptprmpromotesnonsmallcelllungcancerprogressionbymodulatingthemir1395psetd5axis AT hanlinzoubm circrnaptprmpromotesnonsmallcelllungcancerprogressionbymodulatingthemir1395psetd5axis AT kaicancaimd circrnaptprmpromotesnonsmallcelllungcancerprogressionbymodulatingthemir1395psetd5axis |