Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis.
Infection, survival, and proliferation of pathogenic bacteria in humans depend on their capacity to impair host responses and acquire nutrients in a hostile environment. Among such nutrients is heme, a co-factor for oxygen storage, electron transport, photosynthesis, and redox biochemistry, which is...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-05-01
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| Series: | PLoS Pathogens |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19424422/?tool=EBI |
| _version_ | 1818435625590718464 |
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| author | Halina Wójtowicz Tibisay Guevara Cynthia Tallant Mariusz Olczak Aneta Sroka Jan Potempa Maria Solà Teresa Olczak F Xavier Gomis-Rüth |
| author_facet | Halina Wójtowicz Tibisay Guevara Cynthia Tallant Mariusz Olczak Aneta Sroka Jan Potempa Maria Solà Teresa Olczak F Xavier Gomis-Rüth |
| author_sort | Halina Wójtowicz |
| collection | DOAJ |
| description | Infection, survival, and proliferation of pathogenic bacteria in humans depend on their capacity to impair host responses and acquire nutrients in a hostile environment. Among such nutrients is heme, a co-factor for oxygen storage, electron transport, photosynthesis, and redox biochemistry, which is indispensable for life. Porphyromonas gingivalis is the major human bacterial pathogen responsible for severe periodontitis. It recruits heme through HmuY, which sequesters heme from host carriers and delivers it to its cognate outer-membrane transporter, the TonB-dependent receptor HmuR. Here we report that heme binding does not significantly affect the secondary structure of HmuY. The crystal structure of heme-bound HmuY reveals a new all-beta fold mimicking a right hand. The thumb and fingers pinch heme iron through two apical histidine residues, giving rise to highly symmetric octahedral iron co-ordination. The tetrameric quaternary arrangement of the protein found in the crystal structure is consistent with experiments in solution. It shows that thumbs and fingertips, and, by extension, the bound heme groups, are shielded from competing heme-binding proteins from the host. This may also facilitate heme transport to HmuR for internalization. HmuY, both in its apo- and in its heme-bound forms, is resistant to proteolytic digestion by trypsin and the major secreted proteases of P. gingivalis, gingipains K and R. It is also stable against thermal and chemical denaturation. In conclusion, these studies reveal novel molecular properties of HmuY that are consistent with its role as a putative virulence factor during bacterial infection. |
| first_indexed | 2024-12-14T16:55:52Z |
| format | Article |
| id | doaj.art-5401babd099e4124bfdf956a81364648 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-14T16:55:52Z |
| publishDate | 2009-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-5401babd099e4124bfdf956a813646482022-12-21T22:53:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742009-05-0155e100041910.1371/journal.ppat.1000419Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis.Halina WójtowiczTibisay GuevaraCynthia TallantMariusz OlczakAneta SrokaJan PotempaMaria SolàTeresa OlczakF Xavier Gomis-RüthInfection, survival, and proliferation of pathogenic bacteria in humans depend on their capacity to impair host responses and acquire nutrients in a hostile environment. Among such nutrients is heme, a co-factor for oxygen storage, electron transport, photosynthesis, and redox biochemistry, which is indispensable for life. Porphyromonas gingivalis is the major human bacterial pathogen responsible for severe periodontitis. It recruits heme through HmuY, which sequesters heme from host carriers and delivers it to its cognate outer-membrane transporter, the TonB-dependent receptor HmuR. Here we report that heme binding does not significantly affect the secondary structure of HmuY. The crystal structure of heme-bound HmuY reveals a new all-beta fold mimicking a right hand. The thumb and fingers pinch heme iron through two apical histidine residues, giving rise to highly symmetric octahedral iron co-ordination. The tetrameric quaternary arrangement of the protein found in the crystal structure is consistent with experiments in solution. It shows that thumbs and fingertips, and, by extension, the bound heme groups, are shielded from competing heme-binding proteins from the host. This may also facilitate heme transport to HmuR for internalization. HmuY, both in its apo- and in its heme-bound forms, is resistant to proteolytic digestion by trypsin and the major secreted proteases of P. gingivalis, gingipains K and R. It is also stable against thermal and chemical denaturation. In conclusion, these studies reveal novel molecular properties of HmuY that are consistent with its role as a putative virulence factor during bacterial infection.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19424422/?tool=EBI |
| spellingShingle | Halina Wójtowicz Tibisay Guevara Cynthia Tallant Mariusz Olczak Aneta Sroka Jan Potempa Maria Solà Teresa Olczak F Xavier Gomis-Rüth Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. PLoS Pathogens |
| title | Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. |
| title_full | Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. |
| title_fullStr | Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. |
| title_full_unstemmed | Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. |
| title_short | Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. |
| title_sort | unique structure and stability of hmuy a novel heme binding protein of porphyromonas gingivalis |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19424422/?tool=EBI |
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