Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen
The structure of hapten determines the performance of the antibody and the corresponding detection method. A new type of antigen was designed and synthesized to expose the spatial and characteristic structure of dinotefuran molecule, and a type of high-quality antibody was obtained. The IC<sub>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/13/2426 |
| _version_ | 1811269677485129728 |
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| author | Fangfang Zhao Jingkun Liu Jinhui Luo |
| author_facet | Fangfang Zhao Jingkun Liu Jinhui Luo |
| author_sort | Fangfang Zhao |
| collection | DOAJ |
| description | The structure of hapten determines the performance of the antibody and the corresponding detection method. A new type of antigen was designed and synthesized to expose the spatial and characteristic structure of dinotefuran molecule, and a type of high-quality antibody was obtained. The IC<sub>50</sub> value of the monoclonal antibody was 5.30 ng/mL and its cross-reactivity (CRs) was less than 2% when reacting with other structurally related analytes. The effects of spatial configurations of hapten on the antibody were visually analyzed while using the appropriate software according to the quality of the antibodies, which showed that the specificity of the antibody is closely related with the exposed structure of hapten. An ELISA assay with an IC<sub>50</sub> of 5.66 ng/mL and a linear range of 1.95 to 16.29 ng/mL was developed. The results that were obtained from the ELISA and HPLC methods were equivalent. The results showed that spatial simulation is a crucial method that is used in the designing of hapten to obtain a sensitive and specific antibody. The application of this method will highlight the potential aim and improve the detection efficiency of ELISA. |
| first_indexed | 2024-04-12T21:46:28Z |
| format | Article |
| id | doaj.art-54074d41751d4bef9fb80d598a3531f9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T21:46:28Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-54074d41751d4bef9fb80d598a3531f92022-12-22T03:15:36ZengMDPI AGMolecules1420-30492019-07-012413242610.3390/molecules24132426molecules24132426Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed AntigenFangfang Zhao0Jingkun Liu1Jinhui Luo2Analysis & Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, ChinaInstitute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, ChinaAnalysis & Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, ChinaThe structure of hapten determines the performance of the antibody and the corresponding detection method. A new type of antigen was designed and synthesized to expose the spatial and characteristic structure of dinotefuran molecule, and a type of high-quality antibody was obtained. The IC<sub>50</sub> value of the monoclonal antibody was 5.30 ng/mL and its cross-reactivity (CRs) was less than 2% when reacting with other structurally related analytes. The effects of spatial configurations of hapten on the antibody were visually analyzed while using the appropriate software according to the quality of the antibodies, which showed that the specificity of the antibody is closely related with the exposed structure of hapten. An ELISA assay with an IC<sub>50</sub> of 5.66 ng/mL and a linear range of 1.95 to 16.29 ng/mL was developed. The results that were obtained from the ELISA and HPLC methods were equivalent. The results showed that spatial simulation is a crucial method that is used in the designing of hapten to obtain a sensitive and specific antibody. The application of this method will highlight the potential aim and improve the detection efficiency of ELISA.https://www.mdpi.com/1420-3049/24/13/2426dinotefuranHapten designspatial configurationsELISAsensitivity and specificity |
| spellingShingle | Fangfang Zhao Jingkun Liu Jinhui Luo Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen Molecules dinotefuran Hapten design spatial configurations ELISA sensitivity and specificity |
| title | Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen |
| title_full | Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen |
| title_fullStr | Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen |
| title_full_unstemmed | Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen |
| title_short | Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen |
| title_sort | development of a high quality elisa method for dinotefuran based on a novel and newly designed antigen |
| topic | dinotefuran Hapten design spatial configurations ELISA sensitivity and specificity |
| url | https://www.mdpi.com/1420-3049/24/13/2426 |
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