The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids
Viral coat protein (CP) has numerous critical functions in plant infection, but little is known about p25, the CP of maize chlorotic mottle virus (MCMV; Machlomovirus), which causes severe yield losses in maize worldwide. Here, we investigated the roles of p25 in pathogenicity and systemic movement,...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.951479/full |
| _version_ | 1811220587844993024 |
|---|---|
| author | Chao Zhang Di Wang Weimin Li Baolong Zhang Gamal M. Abdel-Fattah Ouf Xiaofeng Su Jun Li |
| author_facet | Chao Zhang Di Wang Weimin Li Baolong Zhang Gamal M. Abdel-Fattah Ouf Xiaofeng Su Jun Li |
| author_sort | Chao Zhang |
| collection | DOAJ |
| description | Viral coat protein (CP) has numerous critical functions in plant infection, but little is known about p25, the CP of maize chlorotic mottle virus (MCMV; Machlomovirus), which causes severe yield losses in maize worldwide. Here, we investigated the roles of p25 in pathogenicity and systemic movement, as well as potential interactions with host plants, using a hybrid tobacco mosaic virus (TMV)-based expression system. Highly conserved protein p25 is predicted to contain a membrane-anchored nuclear localization signal (NLS) sequence and an extracellular sequence. In transgenic Nicotiana benthamiana plants containing the movement protein (MP) of TMV (TMV-MP), p25 induced severe symptoms, including dwarf and foliar necrosis, and was detected in inoculated and non-inoculated leaves. After the deletion of NLS from nuclear-located p25, the protein was found throughout the host cell, and plant stunting and starch granule deformity were reduced. Systemic movement and pathogenicity were significantly impaired when the C-terminal regions of p25 were absent. Using virus-induced gene silencing (VIGS), the transcript level of heat shock protein HSP90 was distinctly lower in host plants in association with the absence of leaf necrosis induced by TMV-p25. Our results revealed crucial roles for MCMV p25 in viral pathogenicity, long-distance movement, and interactions with N. benthamiana. |
| first_indexed | 2024-04-12T07:44:29Z |
| format | Article |
| id | doaj.art-5409db2cfe4443e0833e2bbe4b9bb2a8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T07:44:29Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5409db2cfe4443e0833e2bbe4b9bb2a82022-12-22T03:41:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.951479951479The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybridsChao Zhang0Di Wang1Weimin Li2Baolong Zhang3Gamal M. Abdel-Fattah Ouf4Xiaofeng Su5Jun Li6State Key Laboratory of North China Crop Improvement and Regulation, College of Life Sciences, Hebei Agricultural University, Baoding, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaExcellence and Innovation Center, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Botany and Applied Microbiology, Faculty of Science, Mansoura University, Mansoura, EgyptBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of North China Crop Improvement and Regulation, College of Life Sciences, Hebei Agricultural University, Baoding, ChinaViral coat protein (CP) has numerous critical functions in plant infection, but little is known about p25, the CP of maize chlorotic mottle virus (MCMV; Machlomovirus), which causes severe yield losses in maize worldwide. Here, we investigated the roles of p25 in pathogenicity and systemic movement, as well as potential interactions with host plants, using a hybrid tobacco mosaic virus (TMV)-based expression system. Highly conserved protein p25 is predicted to contain a membrane-anchored nuclear localization signal (NLS) sequence and an extracellular sequence. In transgenic Nicotiana benthamiana plants containing the movement protein (MP) of TMV (TMV-MP), p25 induced severe symptoms, including dwarf and foliar necrosis, and was detected in inoculated and non-inoculated leaves. After the deletion of NLS from nuclear-located p25, the protein was found throughout the host cell, and plant stunting and starch granule deformity were reduced. Systemic movement and pathogenicity were significantly impaired when the C-terminal regions of p25 were absent. Using virus-induced gene silencing (VIGS), the transcript level of heat shock protein HSP90 was distinctly lower in host plants in association with the absence of leaf necrosis induced by TMV-p25. Our results revealed crucial roles for MCMV p25 in viral pathogenicity, long-distance movement, and interactions with N. benthamiana.https://www.frontiersin.org/articles/10.3389/fmicb.2022.951479/fullMCMVp25pathogenicitysystemic movementHSP90 |
| spellingShingle | Chao Zhang Di Wang Weimin Li Baolong Zhang Gamal M. Abdel-Fattah Ouf Xiaofeng Su Jun Li The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids Frontiers in Microbiology MCMV p25 pathogenicity systemic movement HSP90 |
| title | The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| title_full | The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| title_fullStr | The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| title_full_unstemmed | The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| title_short | The coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| title_sort | coat protein p25 from maize chlorotic mottle virus involved in symptom development and systemic movement of tobacco mosaic virus hybrids |
| topic | MCMV p25 pathogenicity systemic movement HSP90 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.951479/full |
| work_keys_str_mv | AT chaozhang thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT diwang thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT weiminli thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT baolongzhang thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT gamalmabdelfattahouf thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT xiaofengsu thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT junli thecoatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT chaozhang coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT diwang coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT weiminli coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT baolongzhang coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT gamalmabdelfattahouf coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT xiaofengsu coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids AT junli coatproteinp25frommaizechloroticmottlevirusinvolvedinsymptomdevelopmentandsystemicmovementoftobaccomosaicvirushybrids |