Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM
Abstract The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In th...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-11-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-023-01298-9 |
| _version_ | 1797630017968013312 |
|---|---|
| author | Chongyi Shu Yiqi Yu Xiaopan Chen Jiansheng Guo Yier Zhou Dandan Wu Tianyun Yang Yuhang Fan Qiongxiao Huang Jing Shu |
| author_facet | Chongyi Shu Yiqi Yu Xiaopan Chen Jiansheng Guo Yier Zhou Dandan Wu Tianyun Yang Yuhang Fan Qiongxiao Huang Jing Shu |
| author_sort | Chongyi Shu |
| collection | DOAJ |
| description | Abstract The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature. |
| first_indexed | 2024-03-11T11:02:08Z |
| format | Article |
| id | doaj.art-540ac97ef6b547388ecbd0ade61ec803 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-03-11T11:02:08Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-540ac97ef6b547388ecbd0ade61ec8032023-11-12T12:26:55ZengBMCJournal of Ovarian Research1757-22152023-11-0116111110.1186/s13048-023-01298-9Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEMChongyi Shu0Yiqi Yu1Xiaopan Chen2Jiansheng Guo3Yier Zhou4Dandan Wu5Tianyun Yang6Yuhang Fan7Qiongxiao Huang8Jing Shu9Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)School of Medicine, Zhejiang UniversityCenter for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Department of Obstetrics, Zhejiang HospitalCenter for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Abstract The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature.https://doi.org/10.1186/s13048-023-01298-9Oocyte cumulus complexGranulosa cellsFocused ion beam scanning electronmicroscopy3D reconstructionOrganellesIntercellular connections |
| spellingShingle | Chongyi Shu Yiqi Yu Xiaopan Chen Jiansheng Guo Yier Zhou Dandan Wu Tianyun Yang Yuhang Fan Qiongxiao Huang Jing Shu Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM Journal of Ovarian Research Oocyte cumulus complex Granulosa cells Focused ion beam scanning electronmicroscopy 3D reconstruction Organelles Intercellular connections |
| title | Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM |
| title_full | Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM |
| title_fullStr | Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM |
| title_full_unstemmed | Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM |
| title_short | Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM |
| title_sort | three dimensional architecture of granulosa cell derived from oocyte cumulus complex revealed by fib sem |
| topic | Oocyte cumulus complex Granulosa cells Focused ion beam scanning electronmicroscopy 3D reconstruction Organelles Intercellular connections |
| url | https://doi.org/10.1186/s13048-023-01298-9 |
| work_keys_str_mv | AT chongyishu threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT yiqiyu threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT xiaopanchen threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT jianshengguo threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT yierzhou threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT dandanwu threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT tianyunyang threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT yuhangfan threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT qiongxiaohuang threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem AT jingshu threedimensionalarchitectureofgranulosacellderivedfromoocytecumuluscomplexrevealedbyfibsem |