Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression?
Nonalcoholic fatty liver disease (NAFLD) is becoming a major public health problem worldwide. The study aimed to evaluate the concentration of eicosanoids in serum and liver tissue during steatosis progression and to assess whether eicosanoid change scores may predict liver tissue remodeling. Thirty...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/9/2026 |
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| author | Dominika Maciejewska Arleta Drozd Karolina Skonieczna-Żydecka Marta Skórka-Majewicz Karolina Dec Karolina Jakubczyk Anna Pilutin Ewa Stachowska |
| author_facet | Dominika Maciejewska Arleta Drozd Karolina Skonieczna-Żydecka Marta Skórka-Majewicz Karolina Dec Karolina Jakubczyk Anna Pilutin Ewa Stachowska |
| author_sort | Dominika Maciejewska |
| collection | DOAJ |
| description | Nonalcoholic fatty liver disease (NAFLD) is becoming a major public health problem worldwide. The study aimed to evaluate the concentration of eicosanoids in serum and liver tissue during steatosis progression and to assess whether eicosanoid change scores may predict liver tissue remodeling. Thirty six eight-week-old male Sprague Dawley rats were enrolled and sacrificed at different stages of NAFLD. Eicosanoid concentrations, namely lipoxin A<sub>4</sub>, hydroxyeicosatetraenoic acids (HETE), hydroxyloctadecadienoic acids (HODE), protectin DX, Maresine1, leucotriene B<sub>4</sub>, prostaglandin E<sub>2</sub>, and resolvin D<sub>1</sub> measurement in serum and liver tissue with Agilent Technologies 1260 liquid chromatography were evaluated. For the liver and serum concentrations of 9-HODE and 13-HODE, the correlations were found to be strong and positive (r > 0.7, <i>p</i> < 0.05). Along with NAFLD progression, HODE concentration significantly increased, and change scores were more abundant in the liver. The moderate positive correlation between liver and serum (r = 0.52, <i>p</i> < 0.05) was also observed for resolvin E1. The eicosanoid concentration decreased during NAFLD progression, but mostly in serum. There were significant correlations between HETE concentrations in liver and serum, but their associations were relatively low and changes the most in liver tissue. Eicosanoids profile, predominantly 9-HODE and 13-HODE, may serve as a potential biomarker for NAFLD development. |
| first_indexed | 2024-03-10T20:12:19Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:12:19Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-540c00e5be3b47db9a1c94f40427e1f32023-11-19T22:49:01ZengMDPI AGMolecules1420-30492020-04-01259202610.3390/molecules25092026Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression?Dominika Maciejewska0Arleta Drozd1Karolina Skonieczna-Żydecka2Marta Skórka-Majewicz3Karolina Dec4Karolina Jakubczyk5Anna Pilutin6Ewa Stachowska7Department of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Histology and Embryology, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandDepartment of Human Nutrition and Metabolomics, Pomeranian Medical University in Szczecin, 70-204 Szczecin, PolandNonalcoholic fatty liver disease (NAFLD) is becoming a major public health problem worldwide. The study aimed to evaluate the concentration of eicosanoids in serum and liver tissue during steatosis progression and to assess whether eicosanoid change scores may predict liver tissue remodeling. Thirty six eight-week-old male Sprague Dawley rats were enrolled and sacrificed at different stages of NAFLD. Eicosanoid concentrations, namely lipoxin A<sub>4</sub>, hydroxyeicosatetraenoic acids (HETE), hydroxyloctadecadienoic acids (HODE), protectin DX, Maresine1, leucotriene B<sub>4</sub>, prostaglandin E<sub>2</sub>, and resolvin D<sub>1</sub> measurement in serum and liver tissue with Agilent Technologies 1260 liquid chromatography were evaluated. For the liver and serum concentrations of 9-HODE and 13-HODE, the correlations were found to be strong and positive (r > 0.7, <i>p</i> < 0.05). Along with NAFLD progression, HODE concentration significantly increased, and change scores were more abundant in the liver. The moderate positive correlation between liver and serum (r = 0.52, <i>p</i> < 0.05) was also observed for resolvin E1. The eicosanoid concentration decreased during NAFLD progression, but mostly in serum. There were significant correlations between HETE concentrations in liver and serum, but their associations were relatively low and changes the most in liver tissue. Eicosanoids profile, predominantly 9-HODE and 13-HODE, may serve as a potential biomarker for NAFLD development.https://www.mdpi.com/1420-3049/25/9/2026eicosanoidsNAFLDNASH9-HODE13-HODEbiomarkers |
| spellingShingle | Dominika Maciejewska Arleta Drozd Karolina Skonieczna-Żydecka Marta Skórka-Majewicz Karolina Dec Karolina Jakubczyk Anna Pilutin Ewa Stachowska Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? Molecules eicosanoids NAFLD NASH 9-HODE 13-HODE biomarkers |
| title | Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? |
| title_full | Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? |
| title_fullStr | Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? |
| title_full_unstemmed | Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? |
| title_short | Eicosanoids in Nonalcoholic Fatty Liver Disease (NAFLD) Progression. Do Serum Eicosanoids Profile Correspond with Liver Eicosanoids Content during NAFLD Development and Progression? |
| title_sort | eicosanoids in nonalcoholic fatty liver disease nafld progression do serum eicosanoids profile correspond with liver eicosanoids content during nafld development and progression |
| topic | eicosanoids NAFLD NASH 9-HODE 13-HODE biomarkers |
| url | https://www.mdpi.com/1420-3049/25/9/2026 |
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