Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection
<p>Abstract</p> <p>Background</p> <p>Despite its widespread use to assess fibrosis, liver biopsy has several important drawbacks, including that is it semi-quantitative, invasive, and limited by sampling and observer variability. Non-invasive serum biomarkers may more a...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-07-01
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| Series: | Journal of Translational Medicine |
| Online Access: | http://www.translational-medicine.com/content/5/1/33 |
| _version_ | 1811297183404654592 |
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| author | Gattu Mahanandeeshwar Cutler Paul Zekry Amany Liu Chiang Skehel Mark Tsokanas Nikos Griffin Philip Dev Anouk Symonds William T Patel Keyur White Ian R Rockey Don C Berrey Michelle M McHutchison John G |
| author_facet | Gattu Mahanandeeshwar Cutler Paul Zekry Amany Liu Chiang Skehel Mark Tsokanas Nikos Griffin Philip Dev Anouk Symonds William T Patel Keyur White Ian R Rockey Don C Berrey Michelle M McHutchison John G |
| author_sort | Gattu Mahanandeeshwar |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Despite its widespread use to assess fibrosis, liver biopsy has several important drawbacks, including that is it semi-quantitative, invasive, and limited by sampling and observer variability. Non-invasive serum biomarkers may more accurately reflect the fibrogenetic process. To identify potential biomarkers of fibrosis, we compared serum protein expression profiles in patients with chronic hepatitis C (CHC) virus infection and fibrosis.</p> <p>Methods</p> <p>Twenty-one patients with no or mild fibrosis (METAVIR stage F0, F1) and 23 with advanced fibrosis (F3, F4) were retrospectively identified from a pedigreed database of 1600 CHC patients. All samples were carefully phenotyped and matched for age, gender, race, body mass index, genotype, duration of infection, alcohol use, and viral load. Expression profiling was performed in a blinded fashion using a 2D polyacrylamide gel electrophoresis/LC-MS/MS platform. Partial least squares discriminant analysis and likelihood ratio statistics were used to rank individual differences in protein expression between the 2 groups.</p> <p>Results</p> <p>Seven individual protein spots were identified as either significantly increased (α<sub>2</sub>-macroglobulin, haptoglobin, albumin) or decreased (complement C-4, serum retinol binding protein, apolipoprotein A-1, and two isoforms of apolipoprotein A-IV) with advanced fibrosis. Three individual proteins, haptoglobin, apolipoprotein A-1, and α<sub>2</sub>-macroglobulin, are included in existing non-invasive serum marker panels.</p> <p>Conclusion</p> <p>Biomarkers identified through expression profiling may facilitate the development of more accurate marker algorithms to better quantitate hepatic fibrosis and monitor disease progression.</p> |
| first_indexed | 2024-04-13T06:00:24Z |
| format | Article |
| id | doaj.art-540c6086a56f4c5fb5f51cd98b702528 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-13T06:00:24Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-540c6086a56f4c5fb5f51cd98b7025282022-12-22T02:59:27ZengBMCJournal of Translational Medicine1479-58762007-07-01513310.1186/1479-5876-5-33Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infectionGattu MahanandeeshwarCutler PaulZekry AmanyLiu ChiangSkehel MarkTsokanas NikosGriffin PhilipDev AnoukSymonds William TPatel KeyurWhite Ian RRockey Don CBerrey Michelle MMcHutchison John G<p>Abstract</p> <p>Background</p> <p>Despite its widespread use to assess fibrosis, liver biopsy has several important drawbacks, including that is it semi-quantitative, invasive, and limited by sampling and observer variability. Non-invasive serum biomarkers may more accurately reflect the fibrogenetic process. To identify potential biomarkers of fibrosis, we compared serum protein expression profiles in patients with chronic hepatitis C (CHC) virus infection and fibrosis.</p> <p>Methods</p> <p>Twenty-one patients with no or mild fibrosis (METAVIR stage F0, F1) and 23 with advanced fibrosis (F3, F4) were retrospectively identified from a pedigreed database of 1600 CHC patients. All samples were carefully phenotyped and matched for age, gender, race, body mass index, genotype, duration of infection, alcohol use, and viral load. Expression profiling was performed in a blinded fashion using a 2D polyacrylamide gel electrophoresis/LC-MS/MS platform. Partial least squares discriminant analysis and likelihood ratio statistics were used to rank individual differences in protein expression between the 2 groups.</p> <p>Results</p> <p>Seven individual protein spots were identified as either significantly increased (α<sub>2</sub>-macroglobulin, haptoglobin, albumin) or decreased (complement C-4, serum retinol binding protein, apolipoprotein A-1, and two isoforms of apolipoprotein A-IV) with advanced fibrosis. Three individual proteins, haptoglobin, apolipoprotein A-1, and α<sub>2</sub>-macroglobulin, are included in existing non-invasive serum marker panels.</p> <p>Conclusion</p> <p>Biomarkers identified through expression profiling may facilitate the development of more accurate marker algorithms to better quantitate hepatic fibrosis and monitor disease progression.</p>http://www.translational-medicine.com/content/5/1/33 |
| spellingShingle | Gattu Mahanandeeshwar Cutler Paul Zekry Amany Liu Chiang Skehel Mark Tsokanas Nikos Griffin Philip Dev Anouk Symonds William T Patel Keyur White Ian R Rockey Don C Berrey Michelle M McHutchison John G Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection Journal of Translational Medicine |
| title | Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection |
| title_full | Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection |
| title_fullStr | Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection |
| title_full_unstemmed | Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection |
| title_short | Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection |
| title_sort | serum proteomic analysis focused on fibrosis in patients with hepatitis c virus infection |
| url | http://www.translational-medicine.com/content/5/1/33 |
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