Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize
MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-02-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913602221 |
| _version_ | 1818437512221163520 |
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| author | Jiang-hua YU Yan-xin ZHAO Ya-ting QIN Bing YUE Yong-lian ZHENG Hai-lin XIAO |
| author_facet | Jiang-hua YU Yan-xin ZHAO Ya-ting QIN Bing YUE Yong-lian ZHENG Hai-lin XIAO |
| author_sort | Jiang-hua YU |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.01<P-value<0.05, respectively. Four miRNAs showing >1.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes. |
| first_indexed | 2024-12-14T17:25:51Z |
| format | Article |
| id | doaj.art-540df3579cb84e9eb2b808b9db648b24 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-14T17:25:51Z |
| publishDate | 2013-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-540df3579cb84e9eb2b808b9db648b242022-12-21T22:53:13ZengElsevierJournal of Integrative Agriculture2095-31192013-02-01122229238Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in MaizeJiang-hua YU0Yan-xin ZHAO1Ya-ting QIN2Bing YUE3Yong-lian ZHENG4Hai-lin XIAO5YU Jiang-hua, Tel: +86-27-87282689; National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaCorrespondence XIAO Hai-lin, Mobile: 18627050215; National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R. ChinaMicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.01<P-value<0.05, respectively. Four miRNAs showing >1.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.http://www.sciencedirect.com/science/article/pii/S2095311913602221CMS-SmicroRNAsreal-time PCRSolexa sequencingtarget genemaize |
| spellingShingle | Jiang-hua YU Yan-xin ZHAO Ya-ting QIN Bing YUE Yong-lian ZHENG Hai-lin XIAO Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize Journal of Integrative Agriculture CMS-S microRNAs real-time PCR Solexa sequencing target gene maize |
| title | Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| title_full | Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| title_fullStr | Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| title_full_unstemmed | Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| title_short | Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| title_sort | discovery of micrornas associated with the s type cytoplasmic male sterility in maize |
| topic | CMS-S microRNAs real-time PCR Solexa sequencing target gene maize |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913602221 |
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