Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
Abstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-15164-4 |
| _version_ | 1819127247789883392 |
|---|---|
| author | Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park |
| author_facet | Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park |
| author_sort | Hyeon-Woo Koh |
| collection | DOAJ |
| description | Abstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize arsenite, and investigated its physiological and genomic traits. Its cells were rod-shaped and Gram-negative, and formed small beige-pigmented colonies. 16S rRNA-based phylogenetic analysis indicated that the strain belongs to the genus Herminiimonas and is closely related to Herminiimonas glaciei UMB49T (98.7% of 16S rRNA gene sequence similarity), Herminiimonas arsenicoxydans ULPAs1T (98.4%), and Herminiimonas saxobsidens NS11T (98.4%). Under chemolithoheterotrophic conditions, the strain utilized some organic acids and amino acids as carbon and/or nitrogen sources but not electron sources. Further, the strain grew as a sulfur oxidizer in a complex medium (trypticase soy agar). Unexpectedly, most carbohydrates failed to support its growth as sole carbon sources. Genome sequencing supported these observations, and very few ABC transporters capable of oligo/monosaccharide uptake were identified in the AS8 genome. The genome harbored genes required for the colonization, flagella biosynthesis, urea degradation, and heavy metal and antibiotic resistance. Based on these polyphasic and genomic analyses, we propose that the strain AS8 be named Herminiimonas arsenitoxidans. |
| first_indexed | 2024-12-22T08:08:54Z |
| format | Article |
| id | doaj.art-540f17052803423b8296db701466ece3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T08:08:54Z |
| publishDate | 2017-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-540f17052803423b8296db701466ece32022-12-21T18:33:04ZengNature PortfolioScientific Reports2045-23222017-11-017111210.1038/s41598-017-15164-4Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidansHyeon-Woo Koh0Moonsuk Hur1Myung-Suk Kang2Youn-Bong Ku3Rohit Ghai4Soo-Je Park5Department of Biology, Jeju National University, 102 Jejudaehak-roMicroorganism Resources Division, National Institute of Biological Resources, 42 Hwangyeong-roMicroorganism Resources Division, National Institute of Biological Resources, 42 Hwangyeong-roMicroorganism Resources Division, National Institute of Biological Resources, 42 Hwangyeong-roInstitute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center CAS, Na Sadkach 7Department of Biology, Jeju National University, 102 Jejudaehak-roAbstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize arsenite, and investigated its physiological and genomic traits. Its cells were rod-shaped and Gram-negative, and formed small beige-pigmented colonies. 16S rRNA-based phylogenetic analysis indicated that the strain belongs to the genus Herminiimonas and is closely related to Herminiimonas glaciei UMB49T (98.7% of 16S rRNA gene sequence similarity), Herminiimonas arsenicoxydans ULPAs1T (98.4%), and Herminiimonas saxobsidens NS11T (98.4%). Under chemolithoheterotrophic conditions, the strain utilized some organic acids and amino acids as carbon and/or nitrogen sources but not electron sources. Further, the strain grew as a sulfur oxidizer in a complex medium (trypticase soy agar). Unexpectedly, most carbohydrates failed to support its growth as sole carbon sources. Genome sequencing supported these observations, and very few ABC transporters capable of oligo/monosaccharide uptake were identified in the AS8 genome. The genome harbored genes required for the colonization, flagella biosynthesis, urea degradation, and heavy metal and antibiotic resistance. Based on these polyphasic and genomic analyses, we propose that the strain AS8 be named Herminiimonas arsenitoxidans.https://doi.org/10.1038/s41598-017-15164-4 |
| spellingShingle | Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans Scientific Reports |
| title | Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans |
| title_full | Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans |
| title_fullStr | Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans |
| title_full_unstemmed | Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans |
| title_short | Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans |
| title_sort | physiological and genomic insights into the lifestyle of arsenite oxidizing herminiimonas arsenitoxidans |
| url | https://doi.org/10.1038/s41598-017-15164-4 |
| work_keys_str_mv | AT hyeonwookoh physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans AT moonsukhur physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans AT myungsukkang physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans AT younbongku physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans AT rohitghai physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans AT soojepark physiologicalandgenomicinsightsintothelifestyleofarseniteoxidizingherminiimonasarsenitoxidans |