Adam21 is dispensable for reproductive processes in mice
Background As a group of membrane-anchored proteins, the proteins containing a disintegrin and metalloprotease domain (ADAMs) control many biological processes, especially for male fertility. Mouse Adam21 was previously found to be specifically expressed in the somatic cells and germ cells of testes...
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| Format: | Article |
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PeerJ Inc.
2021-09-01
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| Online Access: | https://peerj.com/articles/12210.pdf |
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| author | Yinghong Chen Chao Liu Yongliang Shang Liying Wang Wei Li Guoping Li |
| author_facet | Yinghong Chen Chao Liu Yongliang Shang Liying Wang Wei Li Guoping Li |
| author_sort | Yinghong Chen |
| collection | DOAJ |
| description | Background As a group of membrane-anchored proteins, the proteins containing a disintegrin and metalloprotease domain (ADAMs) control many biological processes, especially for male fertility. Mouse Adam21 was previously found to be specifically expressed in the somatic cells and germ cells of testes, but its functional role during spermatogenesis and male reproductive processes is still unknown. Methods Adam21-null mice were created using the CRISPR/Cas9 system. Quantitative real-time PCR was used for analyzing of gene expression. Histological, cytological and immunofluorescence staining were performed to analyze the phenotypes of mouse testis and epididymis. Intracellular lipid droplets (LDs) were detected by Oil red O (ORO) staining and BODIPY staining. Fertility and sperm characteristics were also detected. Results Here, we successfully generated an Adam21 conventional knockout mouse model via CRISPR/Cas9 technology so that we can explore its potential role in male reproduction. We found that male mice lacking Adam21 have normal fertility without any detectable defects in spermatogenesis or sperm motility. Histological analysis of the seminiferous epithelium showed no obvious spermatogenesis difference between Adam21-null and wild-type mice. Cytological analysis revealed no detectable defects in meiotic progression, neither Sertoli cells nor Leydig cells displayed any defect compared with that of the control mice. All these results suggest that Adam21 might not be essential for male fertility in mice, and its potential function still needs further investigation. |
| first_indexed | 2024-03-09T06:34:07Z |
| format | Article |
| id | doaj.art-540fd8a27290465d85a51a458530432e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:34:07Z |
| publishDate | 2021-09-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-540fd8a27290465d85a51a458530432e2023-12-03T11:01:34ZengPeerJ Inc.PeerJ2167-83592021-09-019e1221010.7717/peerj.12210Adam21 is dispensable for reproductive processes in miceYinghong Chen0Chao Liu1Yongliang Shang2Liying Wang3Wei Li4Guoping Li5State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, ChinaAdvanced Medical Research Institute, Shandong University, Jinan, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, ChinaThe MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, ChinaBackground As a group of membrane-anchored proteins, the proteins containing a disintegrin and metalloprotease domain (ADAMs) control many biological processes, especially for male fertility. Mouse Adam21 was previously found to be specifically expressed in the somatic cells and germ cells of testes, but its functional role during spermatogenesis and male reproductive processes is still unknown. Methods Adam21-null mice were created using the CRISPR/Cas9 system. Quantitative real-time PCR was used for analyzing of gene expression. Histological, cytological and immunofluorescence staining were performed to analyze the phenotypes of mouse testis and epididymis. Intracellular lipid droplets (LDs) were detected by Oil red O (ORO) staining and BODIPY staining. Fertility and sperm characteristics were also detected. Results Here, we successfully generated an Adam21 conventional knockout mouse model via CRISPR/Cas9 technology so that we can explore its potential role in male reproduction. We found that male mice lacking Adam21 have normal fertility without any detectable defects in spermatogenesis or sperm motility. Histological analysis of the seminiferous epithelium showed no obvious spermatogenesis difference between Adam21-null and wild-type mice. Cytological analysis revealed no detectable defects in meiotic progression, neither Sertoli cells nor Leydig cells displayed any defect compared with that of the control mice. All these results suggest that Adam21 might not be essential for male fertility in mice, and its potential function still needs further investigation.https://peerj.com/articles/12210.pdfAdam21SpermatogenesisMale infertilityADAMs |
| spellingShingle | Yinghong Chen Chao Liu Yongliang Shang Liying Wang Wei Li Guoping Li Adam21 is dispensable for reproductive processes in mice PeerJ Adam21 Spermatogenesis Male infertility ADAMs |
| title | Adam21 is dispensable for reproductive processes in mice |
| title_full | Adam21 is dispensable for reproductive processes in mice |
| title_fullStr | Adam21 is dispensable for reproductive processes in mice |
| title_full_unstemmed | Adam21 is dispensable for reproductive processes in mice |
| title_short | Adam21 is dispensable for reproductive processes in mice |
| title_sort | adam21 is dispensable for reproductive processes in mice |
| topic | Adam21 Spermatogenesis Male infertility ADAMs |
| url | https://peerj.com/articles/12210.pdf |
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