Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis
Deoxynivalenol (DON), a mycotoxin primarily produced by Fusarium graminearum (F. graminearum), is widely present in food and feed, posing great hazards to human and livestock health. In this study, a strain of Acinetobacter pittii (A. pittii) S12 capable of degrading DON was isolated from soil sampl...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323008990 |
| _version_ | 1797683197408968704 |
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| author | Yuxuan Liu Laipeng Xu Ziyao Shi Ruolin Wang Yang Liu Yujie Gong Yadong Tian Xiangtao Kang Xiangli Sun Yanbin Wang |
| author_facet | Yuxuan Liu Laipeng Xu Ziyao Shi Ruolin Wang Yang Liu Yujie Gong Yadong Tian Xiangtao Kang Xiangli Sun Yanbin Wang |
| author_sort | Yuxuan Liu |
| collection | DOAJ |
| description | Deoxynivalenol (DON), a mycotoxin primarily produced by Fusarium graminearum (F. graminearum), is widely present in food and feed, posing great hazards to human and livestock health. In this study, a strain of Acinetobacter pittii (A. pittii) S12 capable of degrading DON was isolated from soil samples and identified through morphological characterization, biochemistry analysis, and 16 S rRNA gene sequencing. The results of HPLC-MS indicated that the degradation products underwent a conversion from [M-H]- to [M+CH3CO], with concomitant transformation of the hydroxyl group into an acetyl moiety. Based on transcriptome sequencing analysis, the acyltransferase encoded by DLK06_RS13370 was predicted to be the pivotal gene responsible for DON biotransformation. The result of molecular docking analysis suggest a high affinity between the enzyme and DON. The recombinant protein encoded by DLK06_RS13370 was expressed in Escherichia coli (E. coli) and demonstrated the capacity to catalyze the conversion of DON into 3-Acetyl-deoxynivalenol (3-ADON), as confirmed by HPLC analysis. In conclusion, our findings confirm that the acyltransferase encoded by DLK06-RS13370 is responsible for the acetylation of DON. This sheds light on the co-occurrence of DON and its acetyl-derivatives in wheat-based products. Data Availability: Not applicable. |
| first_indexed | 2024-03-12T00:11:03Z |
| format | Article |
| id | doaj.art-5418e47df3fa48c1ad290ea177d23fde |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T00:11:03Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-5418e47df3fa48c1ad290ea177d23fde2023-09-16T05:28:38ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01263115395Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysisYuxuan Liu0Laipeng Xu1Ziyao Shi2Ruolin Wang3Yang Liu4Yujie Gong5Yadong Tian6Xiangtao Kang7Xiangli Sun8Yanbin Wang9College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People’s Republic of ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, People’s Republic of China; Corresponding authors.College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, People’s Republic of China; Corresponding authors.Deoxynivalenol (DON), a mycotoxin primarily produced by Fusarium graminearum (F. graminearum), is widely present in food and feed, posing great hazards to human and livestock health. In this study, a strain of Acinetobacter pittii (A. pittii) S12 capable of degrading DON was isolated from soil samples and identified through morphological characterization, biochemistry analysis, and 16 S rRNA gene sequencing. The results of HPLC-MS indicated that the degradation products underwent a conversion from [M-H]- to [M+CH3CO], with concomitant transformation of the hydroxyl group into an acetyl moiety. Based on transcriptome sequencing analysis, the acyltransferase encoded by DLK06_RS13370 was predicted to be the pivotal gene responsible for DON biotransformation. The result of molecular docking analysis suggest a high affinity between the enzyme and DON. The recombinant protein encoded by DLK06_RS13370 was expressed in Escherichia coli (E. coli) and demonstrated the capacity to catalyze the conversion of DON into 3-Acetyl-deoxynivalenol (3-ADON), as confirmed by HPLC analysis. In conclusion, our findings confirm that the acyltransferase encoded by DLK06-RS13370 is responsible for the acetylation of DON. This sheds light on the co-occurrence of DON and its acetyl-derivatives in wheat-based products. Data Availability: Not applicable.http://www.sciencedirect.com/science/article/pii/S0147651323008990Deoxynivalenol3-acetyl-deoxynimoscylenolAcinetobacter pittiiBiological transformationTranscriptome analysis |
| spellingShingle | Yuxuan Liu Laipeng Xu Ziyao Shi Ruolin Wang Yang Liu Yujie Gong Yadong Tian Xiangtao Kang Xiangli Sun Yanbin Wang Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis Ecotoxicology and Environmental Safety Deoxynivalenol 3-acetyl-deoxynimoscylenol Acinetobacter pittii Biological transformation Transcriptome analysis |
| title | Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis |
| title_full | Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis |
| title_fullStr | Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis |
| title_full_unstemmed | Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis |
| title_short | Identification of an Acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3-acetyl-deoxynivalenol by transcriptomic analysis |
| title_sort | identification of an acinetobacter pittii acyltransferase involved in transformation of deoxynivalenol to 3 acetyl deoxynivalenol by transcriptomic analysis |
| topic | Deoxynivalenol 3-acetyl-deoxynimoscylenol Acinetobacter pittii Biological transformation Transcriptome analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323008990 |
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