Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal
Carya cathayensis Sarg meal (CM) is a by-product of the edible kernel during oil manufacture. In order to improve wastes utilization, the CM derived peptides (CMPs) that showed an in vitro radical scavenging ability were firstly prepared by five different hydrolases. Alcalase treatment revealed the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122002301 |
| _version_ | 1827919372760383488 |
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| author | Fujie Yan Qingqing Wang Jialuo Teng Fenghua Wu Zhiping He |
| author_facet | Fujie Yan Qingqing Wang Jialuo Teng Fenghua Wu Zhiping He |
| author_sort | Fujie Yan |
| collection | DOAJ |
| description | Carya cathayensis Sarg meal (CM) is a by-product of the edible kernel during oil manufacture. In order to improve wastes utilization, the CM derived peptides (CMPs) that showed an in vitro radical scavenging ability were firstly prepared by five different hydrolases. Alcalase treatment revealed the highest yield and the optimal conditions were further determined by response surface methodology (RSM), under which the yield reached 35.84%. Simulated gastrointestinal digestion led to an enrichment of low molecular weight (MW) peptides (<3 kDa), which was beneficial for protecting hepatocyte damaged by hydrogen peroxide (H2O2). Furthermore, generated hydrolysates exhibited protective effects on paraquat-induced Caenorhabditis elegans via enhancing expressions of Skinhead-1 (SKN-1) and its downstream target including glutathione S-transferase (GST)-4 and superoxide dismutase (SOD)-3 to diminish oxidative stress. Taken together, our results demonstrated that simple enzymatic hydrolysis of crude protein powder from CM represents an efficient, eco-friendly and economical strategy for producing bioactive peptides, which can be supplemented in nutraceutical products and food preservation. |
| first_indexed | 2024-03-13T03:56:53Z |
| format | Article |
| id | doaj.art-541a30ef35224142b48abf494aa7628b |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-13T03:56:53Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-541a30ef35224142b48abf494aa7628b2023-06-22T05:04:46ZengElsevierCurrent Research in Food Science2665-92712023-01-016100408Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg mealFujie Yan0Qingqing Wang1Jialuo Teng2Fenghua Wu3Zhiping He4Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, ChinaCollege of Food and Health, Zhejiang Agriculture and Forest University, Hangzhou, 311300, Zhejiang, ChinaDepartment of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, ChinaCollege of Food and Health, Zhejiang Agriculture and Forest University, Hangzhou, 311300, Zhejiang, ChinaCollege of Food and Health, Zhejiang Agriculture and Forest University, Hangzhou, 311300, Zhejiang, China; College of Food Science and Engineering, Xinjiang Institute of Technology Xinjiang, Aksu, 843100, China; Corresponding author. College of Food and Health, Zhejiang Agriculture and Forest University, Hangzhou, 311300, Zhejiang, China.Carya cathayensis Sarg meal (CM) is a by-product of the edible kernel during oil manufacture. In order to improve wastes utilization, the CM derived peptides (CMPs) that showed an in vitro radical scavenging ability were firstly prepared by five different hydrolases. Alcalase treatment revealed the highest yield and the optimal conditions were further determined by response surface methodology (RSM), under which the yield reached 35.84%. Simulated gastrointestinal digestion led to an enrichment of low molecular weight (MW) peptides (<3 kDa), which was beneficial for protecting hepatocyte damaged by hydrogen peroxide (H2O2). Furthermore, generated hydrolysates exhibited protective effects on paraquat-induced Caenorhabditis elegans via enhancing expressions of Skinhead-1 (SKN-1) and its downstream target including glutathione S-transferase (GST)-4 and superoxide dismutase (SOD)-3 to diminish oxidative stress. Taken together, our results demonstrated that simple enzymatic hydrolysis of crude protein powder from CM represents an efficient, eco-friendly and economical strategy for producing bioactive peptides, which can be supplemented in nutraceutical products and food preservation.http://www.sciencedirect.com/science/article/pii/S2665927122002301Antioxidant peptideCarya cathayensis Sarg mealProtein hydrolysatePhysicochemical characteristicsCytoprotective effectCaenorhabditis elegans |
| spellingShingle | Fujie Yan Qingqing Wang Jialuo Teng Fenghua Wu Zhiping He Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal Current Research in Food Science Antioxidant peptide Carya cathayensis Sarg meal Protein hydrolysate Physicochemical characteristics Cytoprotective effect Caenorhabditis elegans |
| title | Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal |
| title_full | Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal |
| title_fullStr | Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal |
| title_full_unstemmed | Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal |
| title_short | Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal |
| title_sort | preparation process optimization and evaluation of bioactive peptides from carya cathayensis sarg meal |
| topic | Antioxidant peptide Carya cathayensis Sarg meal Protein hydrolysate Physicochemical characteristics Cytoprotective effect Caenorhabditis elegans |
| url | http://www.sciencedirect.com/science/article/pii/S2665927122002301 |
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