The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h
ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-04-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/35322 |
| _version_ | 1811253004030967808 |
|---|---|
| author | Nathan Gamarra Stephanie L Johnson Michael J Trnka Alma L Burlingame Geeta J Narlikar |
| author_facet | Nathan Gamarra Stephanie L Johnson Michael J Trnka Alma L Burlingame Geeta J Narlikar |
| author_sort | Nathan Gamarra |
| collection | DOAJ |
| description | ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the human ISWI remodeler SNF2h. Further, by single molecule FRET we show that the acidic patch helps control the distance travelled per translocation event. We propose a model in which the acidic patch activates SNF2h by providing a landing pad for the NegC and AutoN auto-inhibitory domains. Interestingly, the INO80 complex is also strongly dependent on the acidic patch for nucleosome sliding, indicating that this substrate feature can regulate remodeling enzymes with substantially different mechanisms. We therefore hypothesize that regulating access to the acidic patch of the nucleosome plays a key role in coordinating the activities of different remodelers in the cell. |
| first_indexed | 2024-04-12T16:43:12Z |
| format | Article |
| id | doaj.art-541b8cfc76be4658bd1e9d44fdef24a5 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:43:12Z |
| publishDate | 2018-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-541b8cfc76be4658bd1e9d44fdef24a52022-12-22T03:24:41ZengeLife Sciences Publications LtdeLife2050-084X2018-04-01710.7554/eLife.35322The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2hNathan Gamarra0https://orcid.org/0000-0002-2430-8662Stephanie L Johnson1Michael J Trnka2https://orcid.org/0000-0002-8808-5146Alma L Burlingame3Geeta J Narlikar4https://orcid.org/0000-0002-1920-0147Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States; Tetrad Graduate Program, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, United StatesDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the human ISWI remodeler SNF2h. Further, by single molecule FRET we show that the acidic patch helps control the distance travelled per translocation event. We propose a model in which the acidic patch activates SNF2h by providing a landing pad for the NegC and AutoN auto-inhibitory domains. Interestingly, the INO80 complex is also strongly dependent on the acidic patch for nucleosome sliding, indicating that this substrate feature can regulate remodeling enzymes with substantially different mechanisms. We therefore hypothesize that regulating access to the acidic patch of the nucleosome plays a key role in coordinating the activities of different remodelers in the cell.https://elifesciences.org/articles/35322ATP-Dependent Chromatin RemodelingISWIINO80smFRETcross-linking mass spectrometrychromatin |
| spellingShingle | Nathan Gamarra Stephanie L Johnson Michael J Trnka Alma L Burlingame Geeta J Narlikar The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h eLife ATP-Dependent Chromatin Remodeling ISWI INO80 smFRET cross-linking mass spectrometry chromatin |
| title | The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h |
| title_full | The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h |
| title_fullStr | The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h |
| title_full_unstemmed | The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h |
| title_short | The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h |
| title_sort | nucleosomal acidic patch relieves auto inhibition by the iswi remodeler snf2h |
| topic | ATP-Dependent Chromatin Remodeling ISWI INO80 smFRET cross-linking mass spectrometry chromatin |
| url | https://elifesciences.org/articles/35322 |
| work_keys_str_mv | AT nathangamarra thenucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT stephanieljohnson thenucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT michaeljtrnka thenucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT almalburlingame thenucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT geetajnarlikar thenucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT nathangamarra nucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT stephanieljohnson nucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT michaeljtrnka nucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT almalburlingame nucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h AT geetajnarlikar nucleosomalacidicpatchrelievesautoinhibitionbytheiswiremodelersnf2h |