Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease
ObjectiveMetabolomics has found extensive applications in the field of neurological diseases, significantly contributing to their diagnosis and treatment. However, there has been limited research applying metabolomics to moyamoya disease (MMD). This study aims to investigate and identify differentia...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2024.1298385/full |
| _version_ | 1827349896508735488 |
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| author | Jin Yu Tongyu Chen Xiang Li Xiang Li Jincao Chen Wei Wei Wei Wei Jianjian Zhang |
| author_facet | Jin Yu Tongyu Chen Xiang Li Xiang Li Jincao Chen Wei Wei Wei Wei Jianjian Zhang |
| author_sort | Jin Yu |
| collection | DOAJ |
| description | ObjectiveMetabolomics has found extensive applications in the field of neurological diseases, significantly contributing to their diagnosis and treatment. However, there has been limited research applying metabolomics to moyamoya disease (MMD). This study aims to investigate and identify differential metabolites associated with MMD.MethodsWe employed a liquid chromatography coupled with mass spectrometry (LC-MS) approach, complemented by univariate and multivariate analyses, to discern metabolic biomarkers in cerebrospinal fluid samples. We then compared these biomarkers between MMD patients and healthy controls (Ctl).ResultsSixteen patients diagnosed with MMD via cerebral angiography and eight healthy controls were enrolled in this study. Comparative analyses, including univariate and multivariate analyses, correlation studies, heatmaps, Volcano Plots, and KEGG pathway enrichment, were performed between MMD patients and controls. As a result, we identified 129 significant differential metabolites in the cerebrospinal fluid between MMD patients and controls. These metabolic biomarkers are associated with various pathways, with notable involvement in purine and pyrimidine metabolism.ConclusionUtilizing an LC-MS-based metabolomics approach holds promise for enhancing the clinical diagnosis of MMD. The identified biomarkers offer potential avenues for the development of novel diagnostic methods for MMD and offer fresh insights into the pathogenesis of the disease. |
| first_indexed | 2024-03-08T00:50:55Z |
| format | Article |
| id | doaj.art-541c8f696c014d2b98a3a002630e39ef |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-03-08T00:50:55Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-541c8f696c014d2b98a3a002630e39ef2024-02-15T04:40:11ZengFrontiers Media S.A.Frontiers in Neurology1664-22952024-02-011510.3389/fneur.2024.12983851298385Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya diseaseJin Yu0Tongyu Chen1Xiang Li2Xiang Li3Jincao Chen4Wei Wei5Wei Wei6Jianjian Zhang7Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaObjectiveMetabolomics has found extensive applications in the field of neurological diseases, significantly contributing to their diagnosis and treatment. However, there has been limited research applying metabolomics to moyamoya disease (MMD). This study aims to investigate and identify differential metabolites associated with MMD.MethodsWe employed a liquid chromatography coupled with mass spectrometry (LC-MS) approach, complemented by univariate and multivariate analyses, to discern metabolic biomarkers in cerebrospinal fluid samples. We then compared these biomarkers between MMD patients and healthy controls (Ctl).ResultsSixteen patients diagnosed with MMD via cerebral angiography and eight healthy controls were enrolled in this study. Comparative analyses, including univariate and multivariate analyses, correlation studies, heatmaps, Volcano Plots, and KEGG pathway enrichment, were performed between MMD patients and controls. As a result, we identified 129 significant differential metabolites in the cerebrospinal fluid between MMD patients and controls. These metabolic biomarkers are associated with various pathways, with notable involvement in purine and pyrimidine metabolism.ConclusionUtilizing an LC-MS-based metabolomics approach holds promise for enhancing the clinical diagnosis of MMD. The identified biomarkers offer potential avenues for the development of novel diagnostic methods for MMD and offer fresh insights into the pathogenesis of the disease.https://www.frontiersin.org/articles/10.3389/fneur.2024.1298385/fullmoyamoya diseasemetabolomicscerebrospinal fluidpurine metabolismpyrimidine metabolism |
| spellingShingle | Jin Yu Tongyu Chen Xiang Li Xiang Li Jincao Chen Wei Wei Wei Wei Jianjian Zhang Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease Frontiers in Neurology moyamoya disease metabolomics cerebrospinal fluid purine metabolism pyrimidine metabolism |
| title | Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| title_full | Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| title_fullStr | Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| title_full_unstemmed | Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| title_short | Liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| title_sort | liquid chromatography coupled to mass spectrometry metabolomic analysis of cerebrospinal fluid revealed the metabolic characteristics of moyamoya disease |
| topic | moyamoya disease metabolomics cerebrospinal fluid purine metabolism pyrimidine metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2024.1298385/full |
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