Clinical importance of serum miRNA levels in breast cancer patients
Abstract There is limited data on the relationship of miRNAs with parameters that may affect surgical management or reflect tumour prognosis. It was aimed to evaluate serum miRNA levels in breast carcinoma cases and reveal the relationship between these levels and prognosis-related factors such as t...
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| Format: | Article |
| Language: | English |
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Springer
2024-01-01
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| Series: | Discover Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s12672-024-00871-y |
| _version_ | 1797276466955681792 |
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| author | Fatih Turkoglu Akin Calisir Bahadir Ozturk |
| author_facet | Fatih Turkoglu Akin Calisir Bahadir Ozturk |
| author_sort | Fatih Turkoglu |
| collection | DOAJ |
| description | Abstract There is limited data on the relationship of miRNAs with parameters that may affect surgical management or reflect tumour prognosis. It was aimed to evaluate serum miRNA levels in breast carcinoma cases and reveal the relationship between these levels and prognosis-related factors such as the histological type of the tumour, estrogen receptor, progesterone receptor, Ki-67 index, HER-2neu, E-cadherin, tumour size, CK5/6, CA15.3 levels, number of tumour foci, number of metastatic lymph nodes, and status of receiving neoadjuvant therapy. Thirty-five patients with a histopathologically confirmed breast carcinoma diagnosis in the case group and 35 healthy individuals in the control group were examined. miR-206, miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-31, miR-21, miR-155, miR-10b, miR-373, miR-520c, miR-210, miR-145, miR-139-5p, miR-195, miR-99a, miR-497 and miR-205 expression levels in the serum of participants were determined using the Polymerase Chain Reaction method. While serum miR-125b and Let-7a expression levels were significantly higher in breast cancer patients, miR-17-5p, miR-125a, miR-200a, miR-34a, miR-21, miR-99a and miR-497 levels were significantly lower in them. The Let-7a expression level had a statistically significant relationship with breast cancer histological type and HER-2neu parameters, miR-17-5p, miR-125b, Let-7a, miR-34a, miR-21 and miR-99a levels with E-cadherin, miR-34a, miR-99a and miR-497 with CA15.3, miR-125b, miR-200a and miR-34a with the number of metastatic lymph nodes, miR-125a with the number of tumour foci and miR-200a with the status of having the neoadjuvant therapy. Serum miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-21, miR-99a and miR-497 expression levels were determined to have predictive and prognostic importance in breast cancer. |
| first_indexed | 2024-03-07T15:28:35Z |
| format | Article |
| id | doaj.art-542223d69458439d96f086770bea7749 |
| institution | Directory Open Access Journal |
| issn | 2730-6011 |
| language | English |
| last_indexed | 2024-03-07T15:28:35Z |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj.art-542223d69458439d96f086770bea77492024-03-05T16:33:49ZengSpringerDiscover Oncology2730-60112024-01-011511910.1007/s12672-024-00871-yClinical importance of serum miRNA levels in breast cancer patientsFatih Turkoglu0Akin Calisir1Bahadir Ozturk2Department of General Surgery, Faculty of Medicine, Selcuk UniversityDepartment of General Surgery, Faculty of Medicine, Selcuk UniversityDepartment of Biochemistry, Faculty of Medicine, Selcuk UniversityAbstract There is limited data on the relationship of miRNAs with parameters that may affect surgical management or reflect tumour prognosis. It was aimed to evaluate serum miRNA levels in breast carcinoma cases and reveal the relationship between these levels and prognosis-related factors such as the histological type of the tumour, estrogen receptor, progesterone receptor, Ki-67 index, HER-2neu, E-cadherin, tumour size, CK5/6, CA15.3 levels, number of tumour foci, number of metastatic lymph nodes, and status of receiving neoadjuvant therapy. Thirty-five patients with a histopathologically confirmed breast carcinoma diagnosis in the case group and 35 healthy individuals in the control group were examined. miR-206, miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-31, miR-21, miR-155, miR-10b, miR-373, miR-520c, miR-210, miR-145, miR-139-5p, miR-195, miR-99a, miR-497 and miR-205 expression levels in the serum of participants were determined using the Polymerase Chain Reaction method. While serum miR-125b and Let-7a expression levels were significantly higher in breast cancer patients, miR-17-5p, miR-125a, miR-200a, miR-34a, miR-21, miR-99a and miR-497 levels were significantly lower in them. The Let-7a expression level had a statistically significant relationship with breast cancer histological type and HER-2neu parameters, miR-17-5p, miR-125b, Let-7a, miR-34a, miR-21 and miR-99a levels with E-cadherin, miR-34a, miR-99a and miR-497 with CA15.3, miR-125b, miR-200a and miR-34a with the number of metastatic lymph nodes, miR-125a with the number of tumour foci and miR-200a with the status of having the neoadjuvant therapy. Serum miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-21, miR-99a and miR-497 expression levels were determined to have predictive and prognostic importance in breast cancer.https://doi.org/10.1007/s12672-024-00871-yBreast cancerMicrornaPrognosisBiomarker |
| spellingShingle | Fatih Turkoglu Akin Calisir Bahadir Ozturk Clinical importance of serum miRNA levels in breast cancer patients Discover Oncology Breast cancer Microrna Prognosis Biomarker |
| title | Clinical importance of serum miRNA levels in breast cancer patients |
| title_full | Clinical importance of serum miRNA levels in breast cancer patients |
| title_fullStr | Clinical importance of serum miRNA levels in breast cancer patients |
| title_full_unstemmed | Clinical importance of serum miRNA levels in breast cancer patients |
| title_short | Clinical importance of serum miRNA levels in breast cancer patients |
| title_sort | clinical importance of serum mirna levels in breast cancer patients |
| topic | Breast cancer Microrna Prognosis Biomarker |
| url | https://doi.org/10.1007/s12672-024-00871-y |
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