Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C
Background: The objective of the present study was to investigate the ability of butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C to modulate ethanol-Induced toxicity and oxidation damage in maternal and fetal tissues of mice. Butanolic extract from leaves of Chrysanthemum...
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| Format: | Article |
| Language: | English |
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"Vikol publishing" ST Kolesnichenko V.V.
2014-05-01
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| Series: | Journal of Stress Physiology & Biochemistry |
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| Online Access: | http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_35-43.pdf |
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| author | Amel Amrani Nassima Boubekri Ouahiba Benaissa Djamila Zama Fadila Benayache Samir Benayache |
| author_facet | Amel Amrani Nassima Boubekri Ouahiba Benaissa Djamila Zama Fadila Benayache Samir Benayache |
| author_sort | Amel Amrani |
| collection | DOAJ |
| description | Background: The objective of the present study was to investigate the ability of butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C to modulate ethanol-Induced toxicity and oxidation damage in maternal and fetal tissues of mice. Butanolic extract from leaves of Chrysanthemum fontanesii (200 mg/Kg per day), vitamin E (100mg/Kg per day) and C (8.3mg/Kg per day) were administered by gavage to groups of pregnant mice from the 6 th to 17 th day of gestation. A number of animals received plant extract, vitamin E and C, also treated with an oral administration of ethanol (0.02ml/g of 25% v/v absolute ethanol in water per day) in same conditions. On day 18 of gestation, pregnant mice were killed, fetus, placenta, fetal liver, liver, kidneys and brain were removed, homogenised and used for determination of lipid peroxidation (LPO) using TBARS method. Embryotoxicity was assessed by counting the number of live and dead fetus and growth retardation.
Results: Severe alterations in all biomarkers were observed after injury with ETOH. ETOH produced significant decreases in fetal weight and significant increases in embryolethality and lipid peroxidation relative to control values. Treatment with Chrysanthemum fontanesii extract, vitamin C and vitamin E resulted in markedly decreased embryolethality and fetal growth retardation, while increased fetal weight were observed.
Conclusion: The butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C protected against ethanol induce fetal and maternal toxicity as revealed by the decrease in the extent of lipid peroxidation. So that butanolic extract from leaves of Chrysanthemum fontanesii posses in vivo antioxidant properties. |
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| institution | Directory Open Access Journal |
| issn | 1997-0838 |
| language | English |
| last_indexed | 2024-12-10T11:49:23Z |
| publishDate | 2014-05-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-54226ca89fd048b7b3e24f07f7444fb12022-12-22T01:49:57Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08382014-05-011023543Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and CAmel Amrani0Nassima Boubekri1Ouahiba Benaissa2Djamila Zama3Fadila Benayache4Samir Benayache5 Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, Algйrie Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, Algйrie Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, Algйrie Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, Algйrie Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, Algйrie Unitй de Recherche Valorisation des Ressources Naturelles, Molйcules Bioactives, Analyses Physicochimiques et Biologiques (VARENBIOMOL), Universitй Constantine 1, Route de Aпn, El Bey, 25000 Constantine, AlgйrieBackground: The objective of the present study was to investigate the ability of butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C to modulate ethanol-Induced toxicity and oxidation damage in maternal and fetal tissues of mice. Butanolic extract from leaves of Chrysanthemum fontanesii (200 mg/Kg per day), vitamin E (100mg/Kg per day) and C (8.3mg/Kg per day) were administered by gavage to groups of pregnant mice from the 6 th to 17 th day of gestation. A number of animals received plant extract, vitamin E and C, also treated with an oral administration of ethanol (0.02ml/g of 25% v/v absolute ethanol in water per day) in same conditions. On day 18 of gestation, pregnant mice were killed, fetus, placenta, fetal liver, liver, kidneys and brain were removed, homogenised and used for determination of lipid peroxidation (LPO) using TBARS method. Embryotoxicity was assessed by counting the number of live and dead fetus and growth retardation. Results: Severe alterations in all biomarkers were observed after injury with ETOH. ETOH produced significant decreases in fetal weight and significant increases in embryolethality and lipid peroxidation relative to control values. Treatment with Chrysanthemum fontanesii extract, vitamin C and vitamin E resulted in markedly decreased embryolethality and fetal growth retardation, while increased fetal weight were observed. Conclusion: The butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C protected against ethanol induce fetal and maternal toxicity as revealed by the decrease in the extent of lipid peroxidation. So that butanolic extract from leaves of Chrysanthemum fontanesii posses in vivo antioxidant properties.http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_35-43.pdfEthanolembryotoxicitylipid peroxidationChrysanthemum fontanesiivitamin Evitamin C. |
| spellingShingle | Amel Amrani Nassima Boubekri Ouahiba Benaissa Djamila Zama Fadila Benayache Samir Benayache Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C Journal of Stress Physiology & Biochemistry Ethanol embryotoxicity lipid peroxidation Chrysanthemum fontanesii vitamin E vitamin C. |
| title | Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C |
| title_full | Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C |
| title_fullStr | Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C |
| title_full_unstemmed | Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C |
| title_short | Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C |
| title_sort | ethanol induced toxicity and lipid peroxidation in pregnant mice protective effects of butanolic extract from leaves of chrysanthemum fontanesii vitamin e and c |
| topic | Ethanol embryotoxicity lipid peroxidation Chrysanthemum fontanesii vitamin E vitamin C. |
| url | http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_35-43.pdf |
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