| Summary: | The aim of the study was to select microbiological inoculants for a specific plant species, i.e., white lupine (<i>Lupinus albus</i> L.), to increase the efficiency of the diazotroph process. The research involved an in vitro assessment of interactions between the symbiotic bacteria (<i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora commercial preparations dedicated to white lupine) and selected endophytes (<i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i>) used for seed coinoculation. In addition, selected morphological traits of plants (the weight and length of aboveground and belowground parts) were examined after the inoculation/coinoculation. The degree of root colonisation by selected endophytes used as individual inoculants and in combination with bacteria of the <i>Bradyrhizobium</i> genus was determined. The diazotrophic parameters were also investigated (nitrogenase activity, the number, and weight of nodules). The results showed no antagonistic interactions have been demonstrated between bacterial strains of the genus <i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora, and the endophytes <i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i> used for the study. The applied coinoculation in vitro had a stimulating effect on the weight of the stems and roots of white lupine causing an average increase of 13% and 28%, respectively. The level of nitrogenase activity in the coinoculation variants increased from 3.5 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup> to an average of 32.34 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup>.
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