The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.)
The aim of the study was to select microbiological inoculants for a specific plant species, i.e., white lupine (<i>Lupinus albus</i> L.), to increase the efficiency of the diazotroph process. The research involved an in vitro assessment of interactions between the symbiotic bacteria (<...
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MDPI AG
2022-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/23/12382 |
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| author | Zyta Waraczewska Alicja Niewiadomska Agnieszka Wolna-Maruwka Hanna Sulewska Anna Budka Agnieszka A. Pilarska |
| author_facet | Zyta Waraczewska Alicja Niewiadomska Agnieszka Wolna-Maruwka Hanna Sulewska Anna Budka Agnieszka A. Pilarska |
| author_sort | Zyta Waraczewska |
| collection | DOAJ |
| description | The aim of the study was to select microbiological inoculants for a specific plant species, i.e., white lupine (<i>Lupinus albus</i> L.), to increase the efficiency of the diazotroph process. The research involved an in vitro assessment of interactions between the symbiotic bacteria (<i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora commercial preparations dedicated to white lupine) and selected endophytes (<i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i>) used for seed coinoculation. In addition, selected morphological traits of plants (the weight and length of aboveground and belowground parts) were examined after the inoculation/coinoculation. The degree of root colonisation by selected endophytes used as individual inoculants and in combination with bacteria of the <i>Bradyrhizobium</i> genus was determined. The diazotrophic parameters were also investigated (nitrogenase activity, the number, and weight of nodules). The results showed no antagonistic interactions have been demonstrated between bacterial strains of the genus <i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora, and the endophytes <i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i> used for the study. The applied coinoculation in vitro had a stimulating effect on the weight of the stems and roots of white lupine causing an average increase of 13% and 28%, respectively. The level of nitrogenase activity in the coinoculation variants increased from 3.5 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup> to an average of 32.34 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup>. |
| first_indexed | 2024-03-09T17:52:14Z |
| format | Article |
| id | doaj.art-54240294b4a74d9cbc72f83a433c2647 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:52:14Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-54240294b4a74d9cbc72f83a433c26472023-11-24T10:35:36ZengMDPI AGApplied Sciences2076-34172022-12-0112231238210.3390/app122312382The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.)Zyta Waraczewska0Alicja Niewiadomska1Agnieszka Wolna-Maruwka2Hanna Sulewska3Anna Budka4Agnieszka A. Pilarska5Manufacturing and Consulting Company ADOB Ltd., Kołodzieja 11, 61-070 Poznań, PolandDepartment of Soil Science and Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-656 Poznań, PolandDepartment of Soil Science and Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-656 Poznań, PolandDepartment of Agronomy, Poznan University of Life Sciences, ul. Dojazd 11, 60-632 Poznań, PolandDepartment of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, PolandDepartment of Hydraulic and Sanitary Engineering, Poznań University of Life Sciences, ul. Piątkowska 94 A, 60-649 Poznań, PolandThe aim of the study was to select microbiological inoculants for a specific plant species, i.e., white lupine (<i>Lupinus albus</i> L.), to increase the efficiency of the diazotroph process. The research involved an in vitro assessment of interactions between the symbiotic bacteria (<i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora commercial preparations dedicated to white lupine) and selected endophytes (<i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i>) used for seed coinoculation. In addition, selected morphological traits of plants (the weight and length of aboveground and belowground parts) were examined after the inoculation/coinoculation. The degree of root colonisation by selected endophytes used as individual inoculants and in combination with bacteria of the <i>Bradyrhizobium</i> genus was determined. The diazotrophic parameters were also investigated (nitrogenase activity, the number, and weight of nodules). The results showed no antagonistic interactions have been demonstrated between bacterial strains of the genus <i>Bradyrhizobium</i> sp. isolated from Nitragina and Nitroflora, and the endophytes <i>Pseudomonas fluorescens</i> or <i>Bacillus subtilis</i> used for the study. The applied coinoculation in vitro had a stimulating effect on the weight of the stems and roots of white lupine causing an average increase of 13% and 28%, respectively. The level of nitrogenase activity in the coinoculation variants increased from 3.5 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup> to an average of 32.34 nMC<sub>2</sub>H<sub>4</sub> plant<sup>−1</sup> h<sup>−1</sup>.https://www.mdpi.com/2076-3417/12/23/12382<i>Pseudomonas fluorescens</i><i>Bacillus subtilis</i><i>Bradyrhizobium</i> sp.diazotrophynodulesplants parameters |
| spellingShingle | Zyta Waraczewska Alicja Niewiadomska Agnieszka Wolna-Maruwka Hanna Sulewska Anna Budka Agnieszka A. Pilarska The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) Applied Sciences <i>Pseudomonas fluorescens</i> <i>Bacillus subtilis</i> <i>Bradyrhizobium</i> sp. diazotrophy nodules plants parameters |
| title | The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) |
| title_full | The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) |
| title_fullStr | The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) |
| title_full_unstemmed | The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) |
| title_short | The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (<i>Lupinus albus</i> L.) |
| title_sort | effect of in vitro coinoculation on the physiological parameters of white lupine plants i lupinus albus i l |
| topic | <i>Pseudomonas fluorescens</i> <i>Bacillus subtilis</i> <i>Bradyrhizobium</i> sp. diazotrophy nodules plants parameters |
| url | https://www.mdpi.com/2076-3417/12/23/12382 |
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