Diagnosis of onychomycosis by trypsin treatment method
<b>Background: </b> Fungal infection of the nails is a common, difficult to treat problem, prevalent worldwide. A discrepancy in the microscopic examination and culture findings can create problems in the diagnosis of this common infection. <b> Aim:</b> This study was desig...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2007-01-01
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Series: | Indian Journal of Dermatology |
Subjects: | |
Online Access: | http://www.e-ijd.org/article.asp?issn=0019-5154;year=2007;volume=52;issue=1;spage=61;epage=63;aulast=Xess |
Summary: | <b>Background: </b> Fungal infection of the nails is a common, difficult to treat problem, prevalent worldwide. A discrepancy in the microscopic examination and culture findings can create problems in the diagnosis of this common infection. <b> Aim:</b> This study was designed to evaluate a new method for accurate diagnosis of onychomycosis. <b> Materials and Methods:</b> Nail samples from 25 patients of suspected onychomycosis were taken. A portion of the samples was treated with 2% trypsin before culturing and the rest was processed by the standard mycological technique. <b> Results:</b> A higher number of culture positive samples were obtained by the trypsin treatment method as compared to the standard technique. <b> Conclusion:</b> Trypsin treatment prior to culture increases the isolation of fungi from nail samples. |
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ISSN: | 0019-5154 |