Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation
The integrated application of nanoparticles and phytohormones was explored in this study as a potentially eco-friendly remediation strategy to mitigate heavy metal toxicity in a bamboo species (<i>Pleioblastus pygmaeus</i>) by utilizing titanium oxide nanoparticles (TiO<sub>2</s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/3/451 |
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| author | Abolghassem Emamverdian Yulong Ding James Barker Guohua Liu Mirza Hasanuzzaman Yang Li Muthusamy Ramakrishnan Farzad Mokhberdoran |
| author_facet | Abolghassem Emamverdian Yulong Ding James Barker Guohua Liu Mirza Hasanuzzaman Yang Li Muthusamy Ramakrishnan Farzad Mokhberdoran |
| author_sort | Abolghassem Emamverdian |
| collection | DOAJ |
| description | The integrated application of nanoparticles and phytohormones was explored in this study as a potentially eco-friendly remediation strategy to mitigate heavy metal toxicity in a bamboo species (<i>Pleioblastus pygmaeus</i>) by utilizing titanium oxide nanoparticles (TiO<sub>2</sub>-NPs) and 24-epibrassinolide (EBL). Hence, an in vitro experiment was performed to evaluate the role of 100 µM TiO<sub>2</sub> NPs and 10<sup>−8</sup> M 24-epibrassinolide individually and in combination under 100 µM Cu and Cd in a completely randomized design using four replicates. Whereas 100 µM of Cu and Cd reduced antioxidant activity, photosynthetic capacity, plant tolerance, and ultimately plant growth, the co-application of 100 µM TiO<sub>2</sub> NPs and 10<sup>−8</sup> M EBL+ heavy metals (Cu and Cd) resulted in a significant increase in plant antioxidant activity (85%), nonenzymatic antioxidant activities (47%), photosynthetic pigments (43%), fluorescence parameters (68%), plant growth (39%), and plant tolerance (41%) and a significant reduction in the contents of malondialdehyde (45%), hydrogen peroxide (36%), superoxide radical (62%), and soluble protein (28%), as well as the percentage of electrolyte leakage (49%), relative to the control. Moreover, heavy metal accumulation and translocation were reduced by TiO<sub>2</sub> NPs and EBL individually and in combination, which could improve bamboo plant tolerance. |
| first_indexed | 2024-03-09T13:52:56Z |
| format | Article |
| id | doaj.art-542e030f89fa44bdac9494759f1e2924 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:52:56Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-542e030f89fa44bdac9494759f1e29242023-11-30T20:47:28ZengMDPI AGAntioxidants2076-39212022-02-0111345110.3390/antiox11030451Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and TranslocationAbolghassem Emamverdian0Yulong Ding1James Barker2Guohua Liu3Mirza Hasanuzzaman4Yang Li5Muthusamy Ramakrishnan6Farzad Mokhberdoran7Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaSchool of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston-upon-Thames, Surrey KT1 2EE, UKCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshDepartment of Mathematical Sciences, Florida Atlantic University, Boca Raton, FL 33431, USACo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaThe integrated application of nanoparticles and phytohormones was explored in this study as a potentially eco-friendly remediation strategy to mitigate heavy metal toxicity in a bamboo species (<i>Pleioblastus pygmaeus</i>) by utilizing titanium oxide nanoparticles (TiO<sub>2</sub>-NPs) and 24-epibrassinolide (EBL). Hence, an in vitro experiment was performed to evaluate the role of 100 µM TiO<sub>2</sub> NPs and 10<sup>−8</sup> M 24-epibrassinolide individually and in combination under 100 µM Cu and Cd in a completely randomized design using four replicates. Whereas 100 µM of Cu and Cd reduced antioxidant activity, photosynthetic capacity, plant tolerance, and ultimately plant growth, the co-application of 100 µM TiO<sub>2</sub> NPs and 10<sup>−8</sup> M EBL+ heavy metals (Cu and Cd) resulted in a significant increase in plant antioxidant activity (85%), nonenzymatic antioxidant activities (47%), photosynthetic pigments (43%), fluorescence parameters (68%), plant growth (39%), and plant tolerance (41%) and a significant reduction in the contents of malondialdehyde (45%), hydrogen peroxide (36%), superoxide radical (62%), and soluble protein (28%), as well as the percentage of electrolyte leakage (49%), relative to the control. Moreover, heavy metal accumulation and translocation were reduced by TiO<sub>2</sub> NPs and EBL individually and in combination, which could improve bamboo plant tolerance.https://www.mdpi.com/2076-3921/11/3/451toxic metals/metalloidnanoparticlesphytohormonesphytoremediationreactive oxygen species |
| spellingShingle | Abolghassem Emamverdian Yulong Ding James Barker Guohua Liu Mirza Hasanuzzaman Yang Li Muthusamy Ramakrishnan Farzad Mokhberdoran Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation Antioxidants toxic metals/metalloid nanoparticles phytohormones phytoremediation reactive oxygen species |
| title | Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation |
| title_full | Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation |
| title_fullStr | Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation |
| title_full_unstemmed | Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation |
| title_short | Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes <i>Pleioblastus pygmaeus</i> Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation |
| title_sort | co application of 24 epibrassinolide and titanium oxide nanoparticles promotes i pleioblastus pygmaeus i plant tolerance to cu and cd toxicity by increasing antioxidant activity and photosynthetic capacity and reducing heavy metal accumulation and translocation |
| topic | toxic metals/metalloid nanoparticles phytohormones phytoremediation reactive oxygen species |
| url | https://www.mdpi.com/2076-3921/11/3/451 |
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