Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-27129-3 |
| _version_ | 1818342254375337984 |
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| author | Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon |
| author_facet | Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon |
| author_sort | Raghuvir Viswanatha |
| collection | DOAJ |
| description | Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines. |
| first_indexed | 2024-12-13T16:11:46Z |
| format | Article |
| id | doaj.art-54334539e93547d7bcc9a97c1c393d4a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-13T16:11:46Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-54334539e93547d7bcc9a97c1c393d4a2022-12-21T23:38:55ZengNature PortfolioNature Communications2041-17232021-11-0112111310.1038/s41467-021-27129-3Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitosRaghuvir Viswanatha0Enzo Mameli1Jonathan Rodiger2Pierre Merckaert3Fabiana Feitosa-Suntheimer4Tonya M. Colpitts5Stephanie E. Mohr6Yanhui Hu7Norbert Perrimon8Department of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, 620 Albany StreetDepartment of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, 620 Albany StreetDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolForward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.https://doi.org/10.1038/s41467-021-27129-3 |
| spellingShingle | Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos Nature Communications |
| title | Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos |
| title_full | Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos |
| title_fullStr | Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos |
| title_full_unstemmed | Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos |
| title_short | Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos |
| title_sort | bioinformatic and cell based tools for pooled crispr knockout screening in mosquitos |
| url | https://doi.org/10.1038/s41467-021-27129-3 |
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