دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة
This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa and the resulted isolates were divided into 40 clinical isolates form burns, wounds , urine and ear inflammation, an...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Anbar
2015-12-01
|
| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_127584_5df44d89e269f8df646f84b0e64ddd20.pdf |
| _version_ | 1797373196360482816 |
|---|---|
| author | Ahmed M. Yuosif Ahmed M. Turkey Ahmed A. Suliaman |
| author_facet | Ahmed M. Yuosif Ahmed M. Turkey Ahmed A. Suliaman |
| author_sort | Ahmed M. Yuosif |
| collection | DOAJ |
| description | This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa and the resulted isolates were divided into 40 clinical isolates form burns, wounds , urine and ear inflammation, and 10 from soil.Antiobiotic sensitivity test were done against 12 antibiotic discs for all 50 selected isolates by disc diffusion method, and the results indicated that all clinical isolates were resistance to three types of antibiotics(Penicillin, Ampicillin, Amoxicilline) while they varied in their resistance to other antibiotics. The soil isolates were 100% sensitive to all antibiotics except for penicillin and ampicillin were resistance with 60%and 70% respectively. Also the molecular variation for these isolates for virulence factors were detected and the characterization of bacteria was confirmed by checking 16SrRNA gene, after the specific primers were designed for each gene of virulence which included pvdE, toxA, and phzM also specific primer for 16SrRNA was designed. The results showed that the characterization of bacteria was confirmed by 16SrRNA gene detection and sequence and the isolates contain the virulence genes had some polymorphism in comparison with those in NCBI. |
| first_indexed | 2024-03-08T18:46:52Z |
| format | Article |
| id | doaj.art-5434374bde1148eb8884b9375242ee00 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:46:52Z |
| publishDate | 2015-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-5434374bde1148eb8884b9375242ee002023-12-28T21:58:13ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032015-12-0193243510.37652/juaps.2015.127584127584دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیةAhmed M. Yuosif0Ahmed M. Turkey1Ahmed A. Suliaman2Ministry of Health - Anbar Health DepartmentUniversity of Anbar - College of ScienceUniversity of Anbar - Center for Desert StudiesThis study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa and the resulted isolates were divided into 40 clinical isolates form burns, wounds , urine and ear inflammation, and 10 from soil.Antiobiotic sensitivity test were done against 12 antibiotic discs for all 50 selected isolates by disc diffusion method, and the results indicated that all clinical isolates were resistance to three types of antibiotics(Penicillin, Ampicillin, Amoxicilline) while they varied in their resistance to other antibiotics. The soil isolates were 100% sensitive to all antibiotics except for penicillin and ampicillin were resistance with 60%and 70% respectively. Also the molecular variation for these isolates for virulence factors were detected and the characterization of bacteria was confirmed by checking 16SrRNA gene, after the specific primers were designed for each gene of virulence which included pvdE, toxA, and phzM also specific primer for 16SrRNA was designed. The results showed that the characterization of bacteria was confirmed by 16SrRNA gene detection and sequence and the isolates contain the virulence genes had some polymorphism in comparison with those in NCBI.https://juaps.uoanbar.edu.iq/article_127584_5df44d89e269f8df646f84b0e64ddd20.pdfmolecular variation study of clinical isolates of pseudomonas aeruginosa forpvdetoxaand phzm genes related with virulence and characterization featurespcrantibiotic resistancepseudomonas aeruginosa |
| spellingShingle | Ahmed M. Yuosif Ahmed M. Turkey Ahmed A. Suliaman دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة مجلة جامعة الانبار للعلوم الصرفة molecular variation study of clinical isolates of pseudomonas aeruginosa for pvde toxa and phzm genes related with virulence and characterization features pcr antibiotic resistance pseudomonas aeruginosa |
| title | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة |
| title_full | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة |
| title_fullStr | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة |
| title_full_unstemmed | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة |
| title_short | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة |
| title_sort | دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات phzm toxa pvde 16srrna الخاصة بالضراوة والصفات التشخیصیة |
| topic | molecular variation study of clinical isolates of pseudomonas aeruginosa for pvde toxa and phzm genes related with virulence and characterization features pcr antibiotic resistance pseudomonas aeruginosa |
| url | https://juaps.uoanbar.edu.iq/article_127584_5df44d89e269f8df646f84b0e64ddd20.pdf |
| work_keys_str_mv | AT ahmedmyuosif drạsẗạltbạynạljzyỷyllʿzlạtạlmrḍyẗlbḵtryạạlzwạỷfạlznjạryẗljynạtphzmtoxapvde16srrnaạlkẖạṣẗbạlḍrạwẗwạlṣfạtạltsẖkẖyṣyẗ AT ahmedmturkey drạsẗạltbạynạljzyỷyllʿzlạtạlmrḍyẗlbḵtryạạlzwạỷfạlznjạryẗljynạtphzmtoxapvde16srrnaạlkẖạṣẗbạlḍrạwẗwạlṣfạtạltsẖkẖyṣyẗ AT ahmedasuliaman drạsẗạltbạynạljzyỷyllʿzlạtạlmrḍyẗlbḵtryạạlzwạỷfạlznjạryẗljynạtphzmtoxapvde16srrnaạlkẖạṣẗbạlḍrạwẗwạlṣfạtạltsẖkẖyṣyẗ |