Interferometric fluorescence cross correlation spectroscopy.
Measuring transport properties like diffusion and directional flow is essential for understanding dynamics within heterogeneous systems including living cells and novel materials. Fluorescent molecules traveling within these inhomogeneous environments under the forces of Brownian motion and flow exh...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0225797 |
| _version_ | 1818586789330288640 |
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| author | Ipsita Saha Saveez Saffarian |
| author_facet | Ipsita Saha Saveez Saffarian |
| author_sort | Ipsita Saha |
| collection | DOAJ |
| description | Measuring transport properties like diffusion and directional flow is essential for understanding dynamics within heterogeneous systems including living cells and novel materials. Fluorescent molecules traveling within these inhomogeneous environments under the forces of Brownian motion and flow exhibit fluctuations in their concentration, which are directly linked to the transport properties. We present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and under certain conditions, eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels. |
| first_indexed | 2024-12-16T08:58:33Z |
| format | Article |
| id | doaj.art-543668eb37264a718cfe21d53eb0b6d6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:58:33Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-543668eb37264a718cfe21d53eb0b6d62022-12-21T22:37:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022579710.1371/journal.pone.0225797Interferometric fluorescence cross correlation spectroscopy.Ipsita SahaSaveez SaffarianMeasuring transport properties like diffusion and directional flow is essential for understanding dynamics within heterogeneous systems including living cells and novel materials. Fluorescent molecules traveling within these inhomogeneous environments under the forces of Brownian motion and flow exhibit fluctuations in their concentration, which are directly linked to the transport properties. We present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and under certain conditions, eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.https://doi.org/10.1371/journal.pone.0225797 |
| spellingShingle | Ipsita Saha Saveez Saffarian Interferometric fluorescence cross correlation spectroscopy. PLoS ONE |
| title | Interferometric fluorescence cross correlation spectroscopy. |
| title_full | Interferometric fluorescence cross correlation spectroscopy. |
| title_fullStr | Interferometric fluorescence cross correlation spectroscopy. |
| title_full_unstemmed | Interferometric fluorescence cross correlation spectroscopy. |
| title_short | Interferometric fluorescence cross correlation spectroscopy. |
| title_sort | interferometric fluorescence cross correlation spectroscopy |
| url | https://doi.org/10.1371/journal.pone.0225797 |
| work_keys_str_mv | AT ipsitasaha interferometricfluorescencecrosscorrelationspectroscopy AT saveezsaffarian interferometricfluorescencecrosscorrelationspectroscopy |