Improving dental epithelial junction on dental implants with bioengineered peptides
Introduction: The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1165853/full |
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| author | Ivan V. Panayotov Ivan V. Panayotov Attila G. Végh Marta Martin Boyan Vladimirov Christian Larroque Csilla Gergely Frédéric J. G. Cuisinier Frédéric J. G. Cuisinier Elias Estephan Elias Estephan |
| author_facet | Ivan V. Panayotov Ivan V. Panayotov Attila G. Végh Marta Martin Boyan Vladimirov Christian Larroque Csilla Gergely Frédéric J. G. Cuisinier Frédéric J. G. Cuisinier Elias Estephan Elias Estephan |
| author_sort | Ivan V. Panayotov |
| collection | DOAJ |
| description | Introduction: The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported.Results: The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. In vivo tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells.Conclusion: The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice. |
| first_indexed | 2024-03-13T04:20:39Z |
| format | Article |
| id | doaj.art-54367483c62340259b93f1f365906c2b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T04:20:39Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-54367483c62340259b93f1f365906c2b2023-06-20T10:04:20ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-06-011110.3389/fbioe.2023.11658531165853Improving dental epithelial junction on dental implants with bioengineered peptidesIvan V. Panayotov0Ivan V. Panayotov1Attila G. Végh2Marta Martin3Boyan Vladimirov4Christian Larroque5Csilla Gergely6Frédéric J. G. Cuisinier7Frédéric J. G. Cuisinier8Elias Estephan9Elias Estephan10LBN, University Montpellier, Montpellier, FranceCSERD, CHU Montpellier, Montpellier, FranceBiological Research Centre, Institute of Biophysics, Eötvös Lóránd Research Network (ELKH), Szeged, HungaryL2C, University Montpellier, CNRS, Montpellier, FranceDepartment of Maxillofacial Surgery, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Nephrology, CHU Montpellier, Hôpital Lapeyronie, IRMB, University of Montpellier, INSERM U1183, Montpellier, FranceL2C, University Montpellier, CNRS, Montpellier, FranceLBN, University Montpellier, Montpellier, FranceCSERD, CHU Montpellier, Montpellier, FranceLBN, University Montpellier, Montpellier, FranceNeuroscience Research Center, Faculty of Medical Sciences, Lebanese University, Beirut, LebanonIntroduction: The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported.Results: The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. In vivo tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells.Conclusion: The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1165853/fullbioengineered peptidephage displayimplantstitanium surface functionalizationepithelial adhesion |
| spellingShingle | Ivan V. Panayotov Ivan V. Panayotov Attila G. Végh Marta Martin Boyan Vladimirov Christian Larroque Csilla Gergely Frédéric J. G. Cuisinier Frédéric J. G. Cuisinier Elias Estephan Elias Estephan Improving dental epithelial junction on dental implants with bioengineered peptides Frontiers in Bioengineering and Biotechnology bioengineered peptide phage display implants titanium surface functionalization epithelial adhesion |
| title | Improving dental epithelial junction on dental implants with bioengineered peptides |
| title_full | Improving dental epithelial junction on dental implants with bioengineered peptides |
| title_fullStr | Improving dental epithelial junction on dental implants with bioengineered peptides |
| title_full_unstemmed | Improving dental epithelial junction on dental implants with bioengineered peptides |
| title_short | Improving dental epithelial junction on dental implants with bioengineered peptides |
| title_sort | improving dental epithelial junction on dental implants with bioengineered peptides |
| topic | bioengineered peptide phage display implants titanium surface functionalization epithelial adhesion |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1165853/full |
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