A miniature fluorescence microscope for multi-plane imaging
Abstract Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21022-9 |
| _version_ | 1817979424985841664 |
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| author | Giovanni Barbera Rachel Jun Yan Zhang Bo Liang Yun Li Da-Ting Lin |
| author_facet | Giovanni Barbera Rachel Jun Yan Zhang Bo Liang Yun Li Da-Ting Lin |
| author_sort | Giovanni Barbera |
| collection | DOAJ |
| description | Abstract Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by modulating the pulse width of the control signal to a variable focus liquid lens, which is synchronized to the image sensor to enable changing focal plane between frames. The system was tested by imaging GCaMP7f expressing neurons in the mouse medial prefrontal cortex (mPFC) in vivo during open field test. Results showed that with the proposed design it is possible to image neurons across an axial scan of ~ 60 μm, resulting in a ~ 40% increase of total neurons imaged compared to single plane imaging. |
| first_indexed | 2024-04-13T22:42:35Z |
| format | Article |
| id | doaj.art-5438f91cc8e746d5b1ed1dc67f1b53a3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T22:42:35Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5438f91cc8e746d5b1ed1dc67f1b53a32022-12-22T02:26:33ZengNature PortfolioScientific Reports2045-23222022-10-011211810.1038/s41598-022-21022-9A miniature fluorescence microscope for multi-plane imagingGiovanni Barbera0Rachel Jun1Yan Zhang2Bo Liang3Yun Li4Da-Ting Lin5Intramural Research Program, National Institute on Drug Abuse, National Institutes of HealthIntramural Research Program, National Institute on Drug Abuse, National Institutes of HealthIntramural Research Program, National Institute on Drug Abuse, National Institutes of HealthSchool of Electrical Engineering and Computer Science, University of North DakotaDepartment of Zoology and Physiology, University of WyomingIntramural Research Program, National Institute on Drug Abuse, National Institutes of HealthAbstract Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by modulating the pulse width of the control signal to a variable focus liquid lens, which is synchronized to the image sensor to enable changing focal plane between frames. The system was tested by imaging GCaMP7f expressing neurons in the mouse medial prefrontal cortex (mPFC) in vivo during open field test. Results showed that with the proposed design it is possible to image neurons across an axial scan of ~ 60 μm, resulting in a ~ 40% increase of total neurons imaged compared to single plane imaging.https://doi.org/10.1038/s41598-022-21022-9 |
| spellingShingle | Giovanni Barbera Rachel Jun Yan Zhang Bo Liang Yun Li Da-Ting Lin A miniature fluorescence microscope for multi-plane imaging Scientific Reports |
| title | A miniature fluorescence microscope for multi-plane imaging |
| title_full | A miniature fluorescence microscope for multi-plane imaging |
| title_fullStr | A miniature fluorescence microscope for multi-plane imaging |
| title_full_unstemmed | A miniature fluorescence microscope for multi-plane imaging |
| title_short | A miniature fluorescence microscope for multi-plane imaging |
| title_sort | miniature fluorescence microscope for multi plane imaging |
| url | https://doi.org/10.1038/s41598-022-21022-9 |
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