METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway
Abstract Hepatic fibrosis (HF) is caused by chronic hepatic injury and is characterized by hepatic stellate cells (HSCs) activation. Studies focusing on the function of exosomes derived from macrophages in HF progression are limited. This study aims to identify the roles of exosomal NEAT1 derived fr...
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Format: | Article |
Language: | English |
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Nature Publishing Group
2022-05-01
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Series: | Cell Death Discovery |
Online Access: | https://doi.org/10.1038/s41420-022-01036-y |
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author | Bo Shu Rui-Zhi Zhang Ying-Xia Zhou Chao He Xin Yang |
author_facet | Bo Shu Rui-Zhi Zhang Ying-Xia Zhou Chao He Xin Yang |
author_sort | Bo Shu |
collection | DOAJ |
description | Abstract Hepatic fibrosis (HF) is caused by chronic hepatic injury and is characterized by hepatic stellate cells (HSCs) activation. Studies focusing on the function of exosomes derived from macrophages in HF progression are limited. This study aims to identify the roles of exosomal NEAT1 derived from macrophages on HF and the underlying mechanisms. Our studies showed that METTL3 targeted and enhanced NEAT1 expression in macrophages. Exosomal NEAT1 originating from LPS-treated macrophages promoted HSCs proliferation and migration, and induced the expression of fibrotic proteins including collagen I, α-SMA, and fibronectin. Macrophage exosomal NEAT1 contributed to HSCs activation by sponging miR-342. MiR-342 directly targeted Sp1 and suppressed its downstream TGF-β1/Smad signaling pathway, which eventually led to the inhibition of HSCs activation. Depletion of NEAT1 in the macrophage exosomes inhibited HF progression both in vitro and in vivo. Altogether, our study proved that silence of NEAT1 in the macrophage exosomes exerted protective roles against HF through the miR-342/Sp1/TGF-β1/Smad signaling pathway, suggesting a potential therapeutic target in HF treatment. |
first_indexed | 2024-04-13T18:54:31Z |
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id | doaj.art-543afb54e0a1486c91eafb609fdf29b5 |
institution | Directory Open Access Journal |
issn | 2058-7716 |
language | English |
last_indexed | 2024-04-13T18:54:31Z |
publishDate | 2022-05-01 |
publisher | Nature Publishing Group |
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series | Cell Death Discovery |
spelling | doaj.art-543afb54e0a1486c91eafb609fdf29b52022-12-22T02:34:18ZengNature Publishing GroupCell Death Discovery2058-77162022-05-018111410.1038/s41420-022-01036-yMETTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathwayBo Shu0Rui-Zhi Zhang1Ying-Xia Zhou2Chao He3Xin Yang4Department of General Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of General Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Surgical Operation, The Second Xiangya Hospital, Central South UniversityDepartment of General Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of General Surgery, The Second Xiangya Hospital, Central South UniversityAbstract Hepatic fibrosis (HF) is caused by chronic hepatic injury and is characterized by hepatic stellate cells (HSCs) activation. Studies focusing on the function of exosomes derived from macrophages in HF progression are limited. This study aims to identify the roles of exosomal NEAT1 derived from macrophages on HF and the underlying mechanisms. Our studies showed that METTL3 targeted and enhanced NEAT1 expression in macrophages. Exosomal NEAT1 originating from LPS-treated macrophages promoted HSCs proliferation and migration, and induced the expression of fibrotic proteins including collagen I, α-SMA, and fibronectin. Macrophage exosomal NEAT1 contributed to HSCs activation by sponging miR-342. MiR-342 directly targeted Sp1 and suppressed its downstream TGF-β1/Smad signaling pathway, which eventually led to the inhibition of HSCs activation. Depletion of NEAT1 in the macrophage exosomes inhibited HF progression both in vitro and in vivo. Altogether, our study proved that silence of NEAT1 in the macrophage exosomes exerted protective roles against HF through the miR-342/Sp1/TGF-β1/Smad signaling pathway, suggesting a potential therapeutic target in HF treatment.https://doi.org/10.1038/s41420-022-01036-y |
spellingShingle | Bo Shu Rui-Zhi Zhang Ying-Xia Zhou Chao He Xin Yang METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway Cell Death Discovery |
title | METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway |
title_full | METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway |
title_fullStr | METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway |
title_full_unstemmed | METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway |
title_short | METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-β1/Smad signaling pathway |
title_sort | mettl3 mediated macrophage exosomal neat1 contributes to hepatic fibrosis progression through sp1 tgf β1 smad signaling pathway |
url | https://doi.org/10.1038/s41420-022-01036-y |
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