The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit
Auxin plays an important role in regulating plant development, and <i>Auxin/indole acetic acid</i> (<i>Aux/IAA</i>) is a type of auxin-responsive gene and plays an important role in auxin signaling; to date, although 29 Aux/IAA proteins have been reported in <i>Abrabido...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9454 |
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| author | Haidong Bu Xiaohuan Sun Pengtao Yue Junling Qiao Jiamao Sun Aide Wang Hui Yuan Wenquan Yu |
| author_facet | Haidong Bu Xiaohuan Sun Pengtao Yue Junling Qiao Jiamao Sun Aide Wang Hui Yuan Wenquan Yu |
| author_sort | Haidong Bu |
| collection | DOAJ |
| description | Auxin plays an important role in regulating plant development, and <i>Auxin/indole acetic acid</i> (<i>Aux/IAA</i>) is a type of auxin-responsive gene and plays an important role in auxin signaling; to date, although 29 Aux/IAA proteins have been reported in <i>Abrabidopsis thaliana</i>, only parts of the <i>Aux/IAA</i> family gene functions have been identified. We previously reported that a bud sport of ‘Longfeng’ (LF) apple (<i>Malus domestica</i>), named ‘Grand longfeng’ (GLF), which showed a larger fruit size than LF, has lower expression of <i>MdAux/IAA2</i>. In this study, we identified the function of the <i>MdAux/IAA2</i> gene in apple fruit size difference using <i>Agrobacterium</i>-mediated genetic transformation. Overexpression of <i>MdAux/IAA2</i> decreased the apple flesh callus increment and caused a smaller globular cell size. In addition, overexpression of <i>MdAux/IAA2</i> in GLF fruit resulted in the reduction of apple fruit size, weight, and cell size, while silencing <i>MdAux/IAA2</i> in LF apple fruit resulted in an increase in apple fruit weight and cell size. We suggest that the high auxin content depressed the expression of <i>MdAux/IAA2</i>, and that the downregulated expression of <i>MdAux/IAA2</i> led to the formation of GLF. Our study suggests a mechanism for fruit size regulation in plants and we will explore the transcription factors functioning in this process in the future. |
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| language | English |
| last_indexed | 2024-03-09T09:55:16Z |
| publishDate | 2022-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-543d1bf361a94e1bbf07cdad92e7c0182023-12-01T23:48:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316945410.3390/ijms23169454The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple FruitHaidong Bu0Xiaohuan Sun1Pengtao Yue2Junling Qiao3Jiamao Sun4Aide Wang5Hui Yuan6Wenquan Yu7Mudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province), Key Laboratory of Protected Horticulture (Ministry of Education), National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province), Key Laboratory of Protected Horticulture (Ministry of Education), National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province), Key Laboratory of Protected Horticulture (Ministry of Education), National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province), Key Laboratory of Protected Horticulture (Ministry of Education), National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province), Key Laboratory of Protected Horticulture (Ministry of Education), National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaAuxin plays an important role in regulating plant development, and <i>Auxin/indole acetic acid</i> (<i>Aux/IAA</i>) is a type of auxin-responsive gene and plays an important role in auxin signaling; to date, although 29 Aux/IAA proteins have been reported in <i>Abrabidopsis thaliana</i>, only parts of the <i>Aux/IAA</i> family gene functions have been identified. We previously reported that a bud sport of ‘Longfeng’ (LF) apple (<i>Malus domestica</i>), named ‘Grand longfeng’ (GLF), which showed a larger fruit size than LF, has lower expression of <i>MdAux/IAA2</i>. In this study, we identified the function of the <i>MdAux/IAA2</i> gene in apple fruit size difference using <i>Agrobacterium</i>-mediated genetic transformation. Overexpression of <i>MdAux/IAA2</i> decreased the apple flesh callus increment and caused a smaller globular cell size. In addition, overexpression of <i>MdAux/IAA2</i> in GLF fruit resulted in the reduction of apple fruit size, weight, and cell size, while silencing <i>MdAux/IAA2</i> in LF apple fruit resulted in an increase in apple fruit weight and cell size. We suggest that the high auxin content depressed the expression of <i>MdAux/IAA2</i>, and that the downregulated expression of <i>MdAux/IAA2</i> led to the formation of GLF. Our study suggests a mechanism for fruit size regulation in plants and we will explore the transcription factors functioning in this process in the future.https://www.mdpi.com/1422-0067/23/16/9454applefruit sizeauxin<i>MdAux/IAA2</i>cell size |
| spellingShingle | Haidong Bu Xiaohuan Sun Pengtao Yue Junling Qiao Jiamao Sun Aide Wang Hui Yuan Wenquan Yu The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit International Journal of Molecular Sciences apple fruit size auxin <i>MdAux/IAA2</i> cell size |
| title | The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit |
| title_full | The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit |
| title_fullStr | The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit |
| title_full_unstemmed | The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit |
| title_short | The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit |
| title_sort | i mdaux iaa2 i transcription repressor regulates cell and fruit size in apple fruit |
| topic | apple fruit size auxin <i>MdAux/IAA2</i> cell size |
| url | https://www.mdpi.com/1422-0067/23/16/9454 |
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