A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway
Nuanxinkang tablet (NXK), a Chinese herbal formula, can improve heart function and quality of life in patients with chronic heart failure (CHF). However, the mechanisms of action of NXK are not fully understood. In this study, we investigated the effects of NXK on inflammation in the CHF mouse model...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.865614/full |
| _version_ | 1818469880458903552 |
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| author | Xiaoming Dong Xiaowei Han Xiaojiao Zhang Sijing Li Ziru Li Jinhua Kang Jialin Jiang Shihao Ni Lu Lu Zhiling He Zhiling He Haoming Huang Shaoxiang Xian Shaoxiang Xian Tianhui Yuan Zhongqi Yang Zhongqi Yang Wenjie Long Wenjie Long Zemin Wan |
| author_facet | Xiaoming Dong Xiaowei Han Xiaojiao Zhang Sijing Li Ziru Li Jinhua Kang Jialin Jiang Shihao Ni Lu Lu Zhiling He Zhiling He Haoming Huang Shaoxiang Xian Shaoxiang Xian Tianhui Yuan Zhongqi Yang Zhongqi Yang Wenjie Long Wenjie Long Zemin Wan |
| author_sort | Xiaoming Dong |
| collection | DOAJ |
| description | Nuanxinkang tablet (NXK), a Chinese herbal formula, can improve heart function and quality of life in patients with chronic heart failure (CHF). However, the mechanisms of action of NXK are not fully understood. In this study, we investigated the effects of NXK on inflammation in the CHF mouse model. This model was established by transverse aortic constriction (TAC) and treated with NXK for 8 weeks. Then, the cardiac function and myocardial fibrosis were evaluated. The monocytes/macrophages were evaluated by immunofluorescence. The mRNA levels of IL-1β, IL-6, TNF-α, ICAM-1, and VCAM-1 were measured by quantitative real-time polymerase chain reaction (qRT-PCR), while TLR4, MyD88, NF-κB p65, P-IκBα, TLR2, TLR7 and TLR9 protein levels were evaluated by Western blot. The results showed that NXK improved the left ventricular ejection fraction (LVEF) and left ventricular end-systolic dimension, reversed myocardial fibrosis, and inhibited pro-inflammatory (CD11b + Ly6C+) monocytes/macrophages in the TAC mouse model. NXK also reduced the mRNA and protein levels of the above markers. Taken together, NXK improved heart function and reduced inflammation through the TLR-mediated NF-κB signaling pathway, suggesting that it might be used as an innovative treatment strategy for CHF. |
| first_indexed | 2024-04-13T21:30:36Z |
| format | Article |
| id | doaj.art-544130db3a6a4f61818d0c01fe1a2160 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T21:30:36Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-544130db3a6a4f61818d0c01fe1a21602022-12-22T02:29:11ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-06-011310.3389/fphar.2022.865614865614A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling PathwayXiaoming Dong0Xiaowei Han1Xiaojiao Zhang2Sijing Li3Ziru Li4Jinhua Kang5Jialin Jiang6Shihao Ni7Lu Lu8Zhiling He9Zhiling He10Haoming Huang11Shaoxiang Xian12Shaoxiang Xian13Tianhui Yuan14Zhongqi Yang15Zhongqi Yang16Wenjie Long17Wenjie Long18Zemin Wan19The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaLingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Radiology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaLingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaLingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaLingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Geriatrics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaNuanxinkang tablet (NXK), a Chinese herbal formula, can improve heart function and quality of life in patients with chronic heart failure (CHF). However, the mechanisms of action of NXK are not fully understood. In this study, we investigated the effects of NXK on inflammation in the CHF mouse model. This model was established by transverse aortic constriction (TAC) and treated with NXK for 8 weeks. Then, the cardiac function and myocardial fibrosis were evaluated. The monocytes/macrophages were evaluated by immunofluorescence. The mRNA levels of IL-1β, IL-6, TNF-α, ICAM-1, and VCAM-1 were measured by quantitative real-time polymerase chain reaction (qRT-PCR), while TLR4, MyD88, NF-κB p65, P-IκBα, TLR2, TLR7 and TLR9 protein levels were evaluated by Western blot. The results showed that NXK improved the left ventricular ejection fraction (LVEF) and left ventricular end-systolic dimension, reversed myocardial fibrosis, and inhibited pro-inflammatory (CD11b + Ly6C+) monocytes/macrophages in the TAC mouse model. NXK also reduced the mRNA and protein levels of the above markers. Taken together, NXK improved heart function and reduced inflammation through the TLR-mediated NF-κB signaling pathway, suggesting that it might be used as an innovative treatment strategy for CHF.https://www.frontiersin.org/articles/10.3389/fphar.2022.865614/fullchronic heart failureinflammationChinese medicineNuanxinkang tabletTLR-mediated NF-κB signaling pathway |
| spellingShingle | Xiaoming Dong Xiaowei Han Xiaojiao Zhang Sijing Li Ziru Li Jinhua Kang Jialin Jiang Shihao Ni Lu Lu Zhiling He Zhiling He Haoming Huang Shaoxiang Xian Shaoxiang Xian Tianhui Yuan Zhongqi Yang Zhongqi Yang Wenjie Long Wenjie Long Zemin Wan A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway Frontiers in Pharmacology chronic heart failure inflammation Chinese medicine Nuanxinkang tablet TLR-mediated NF-κB signaling pathway |
| title | A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway |
| title_full | A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway |
| title_fullStr | A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway |
| title_full_unstemmed | A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway |
| title_short | A Simplified Herbal Formula Improves Cardiac Function and Reduces Inflammation in Mice Through the TLR-Mediated NF-κB Signaling Pathway |
| title_sort | simplified herbal formula improves cardiac function and reduces inflammation in mice through the tlr mediated nf κb signaling pathway |
| topic | chronic heart failure inflammation Chinese medicine Nuanxinkang tablet TLR-mediated NF-κB signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.865614/full |
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