Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium
Intestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of CD8+ T cells critical for monitoring the MHC I antigen processing pathw...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/90466 |
| _version_ | 1797383202384379904 |
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| author | Jian Guan J David Peske Michael Manoharan Valerio Chansu Park Ellen A Robey Scheherazade Sadegh-Nasseri |
| author_facet | Jian Guan J David Peske Michael Manoharan Valerio Chansu Park Ellen A Robey Scheherazade Sadegh-Nasseri |
| author_sort | Jian Guan |
| collection | DOAJ |
| description | Intestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of CD8+ T cells critical for monitoring the MHC I antigen processing pathway, we established that unconventional Qa-1b-restricted CD8+ T cells are abundant in intestinal epithelium. We found that QFL T cells showed a Qa-1b-dependent unconventional phenotype in the spleen and small intestine of naïve wild-type mice. The splenic QFL T cells showed innate-like functionality exemplified by rapid response to cytokines or antigens, while the gut population was refractory to stimuli. Microbiota was required for the maintenance, but not the initial gut homing of QFL T cells. Moreover, monocolonization with Pediococcus pentosaceus, which expresses a peptide that cross-activated QFL T cells, was sufficient to maintain QFL T cells in the intestine. Thus, microbiota is critical for shaping the Qa-1b-restricted IEL landscape. |
| first_indexed | 2024-03-08T21:17:13Z |
| format | Article |
| id | doaj.art-54430899bea94fd7b55a65b2c274a757 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-08T21:17:13Z |
| publishDate | 2023-12-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj.art-54430899bea94fd7b55a65b2c274a7572023-12-21T15:01:50ZengeLife Sciences Publications LtdeLife2050-084X2023-12-011210.7554/eLife.90466Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epitheliumJian Guan0https://orcid.org/0000-0002-0118-6578J David Peske1Michael Manoharan Valerio2Chansu Park3Ellen A Robey4https://orcid.org/0000-0002-3630-5266Scheherazade Sadegh-Nasseri5https://orcid.org/0000-0002-8127-1720Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, United States; Institute of Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, United StatesDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, United States; Institute of Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, United StatesDivision of Immunology and Molecular Medicine, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, United States; Institute of Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, United StatesDivision of Immunology and Molecular Medicine, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, United StatesIntestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of CD8+ T cells critical for monitoring the MHC I antigen processing pathway, we established that unconventional Qa-1b-restricted CD8+ T cells are abundant in intestinal epithelium. We found that QFL T cells showed a Qa-1b-dependent unconventional phenotype in the spleen and small intestine of naïve wild-type mice. The splenic QFL T cells showed innate-like functionality exemplified by rapid response to cytokines or antigens, while the gut population was refractory to stimuli. Microbiota was required for the maintenance, but not the initial gut homing of QFL T cells. Moreover, monocolonization with Pediococcus pentosaceus, which expresses a peptide that cross-activated QFL T cells, was sufficient to maintain QFL T cells in the intestine. Thus, microbiota is critical for shaping the Qa-1b-restricted IEL landscape.https://elifesciences.org/articles/90466IELunconventional TmicrobiotaMHC-E |
| spellingShingle | Jian Guan J David Peske Michael Manoharan Valerio Chansu Park Ellen A Robey Scheherazade Sadegh-Nasseri Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium eLife IEL unconventional T microbiota MHC-E |
| title | Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium |
| title_full | Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium |
| title_fullStr | Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium |
| title_full_unstemmed | Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium |
| title_short | Commensal bacteria maintain a Qa-1b-restricted unconventional CD8+ T population in gut epithelium |
| title_sort | commensal bacteria maintain a qa 1b restricted unconventional cd8 t population in gut epithelium |
| topic | IEL unconventional T microbiota MHC-E |
| url | https://elifesciences.org/articles/90466 |
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