POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE
Acute Myeloid Leukemia (AML) is a prevalent form of leukemia, responsible for an alarming 62% of all fatalities related to leukemia globally, and the current chemotherapy treatment is known for its considerable negative impacts on patient health. This underscores the potential of the bioactive compo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137923007162 |
| _version_ | 1797654967919575040 |
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| author | IC Rocha RGB Gomes NG Amôr STO Saad MC Alvarez |
| author_facet | IC Rocha RGB Gomes NG Amôr STO Saad MC Alvarez |
| author_sort | IC Rocha |
| collection | DOAJ |
| description | Acute Myeloid Leukemia (AML) is a prevalent form of leukemia, responsible for an alarming 62% of all fatalities related to leukemia globally, and the current chemotherapy treatment is known for its considerable negative impacts on patient health. This underscores the potential of the bioactive compounds found in natural products as vital adjuncts in disease management. In this context, Hesperidin (HD) and its aglycone Hesperetin (HT), polyphenols classified as flavanones, were investigated for their pro-apoptotic properties on the K562 cell line. The MTT assay revealed IC50 values of 200uM for HD and 150uM for HT, reflecting their potential in inhibiting cellular proliferation. Both compounds were found to induce programmed cell death at concentrations of 75, 100, 150, 200, and 300uM, across 24 and 48-hour time exposure. Intriguingly, HD demonstrated a more robust induction of apoptosis compared to HT at equivalent concentrations and duration of exposure, with apoptosis rates of 84% and 89% for 150uM HD at 24 and 48 hours, respectively, versus 32% and 38% for 150uM HT. These preliminary findings suggest that Hesperidin and Hesperetin may hold promise as efficacious initiators of the apoptosis pathway and could be explored as potential adjuvant therapies in the treatment of AML. |
| first_indexed | 2024-03-11T17:07:15Z |
| format | Article |
| id | doaj.art-54443e67614943f690560f71634bc255 |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-03-11T17:07:15Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-54443e67614943f690560f71634bc2552023-10-20T06:42:07ZengElsevierHematology, Transfusion and Cell Therapy2531-13792023-10-0145S270POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINEIC Rocha0RGB Gomes1NG Amôr2STO Saad3MC Alvarez4Centro de Hematologia e Hemoterapia (Hemocentro), Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil; Universidade São Francisco (USF), Bragança Paulista, BrazilCentro de Hematologia e Hemoterapia (Hemocentro), Universidade Estadual de Campinas (UNICAMP), Campinas, BrazilCentro de Hematologia e Hemoterapia (Hemocentro), Universidade Estadual de Campinas (UNICAMP), Campinas, BrazilCentro de Hematologia e Hemoterapia (Hemocentro), Universidade Estadual de Campinas (UNICAMP), Campinas, BrazilCentro de Hematologia e Hemoterapia (Hemocentro), Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil; Universidade São Francisco (USF), Bragança Paulista, BrazilAcute Myeloid Leukemia (AML) is a prevalent form of leukemia, responsible for an alarming 62% of all fatalities related to leukemia globally, and the current chemotherapy treatment is known for its considerable negative impacts on patient health. This underscores the potential of the bioactive compounds found in natural products as vital adjuncts in disease management. In this context, Hesperidin (HD) and its aglycone Hesperetin (HT), polyphenols classified as flavanones, were investigated for their pro-apoptotic properties on the K562 cell line. The MTT assay revealed IC50 values of 200uM for HD and 150uM for HT, reflecting their potential in inhibiting cellular proliferation. Both compounds were found to induce programmed cell death at concentrations of 75, 100, 150, 200, and 300uM, across 24 and 48-hour time exposure. Intriguingly, HD demonstrated a more robust induction of apoptosis compared to HT at equivalent concentrations and duration of exposure, with apoptosis rates of 84% and 89% for 150uM HD at 24 and 48 hours, respectively, versus 32% and 38% for 150uM HT. These preliminary findings suggest that Hesperidin and Hesperetin may hold promise as efficacious initiators of the apoptosis pathway and could be explored as potential adjuvant therapies in the treatment of AML.http://www.sciencedirect.com/science/article/pii/S2531137923007162 |
| spellingShingle | IC Rocha RGB Gomes NG Amôr STO Saad MC Alvarez POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE Hematology, Transfusion and Cell Therapy |
| title | POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE |
| title_full | POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE |
| title_fullStr | POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE |
| title_full_unstemmed | POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE |
| title_short | POTENTIAL EFFECTS OF CITRUS FLAVONOIDS HESPERIDIN AND ITS AGLYCONE HESPERETIN IN LEUKEMIA CELL LINE |
| title_sort | potential effects of citrus flavonoids hesperidin and its aglycone hesperetin in leukemia cell line |
| url | http://www.sciencedirect.com/science/article/pii/S2531137923007162 |
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