Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis
In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an e...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117300255 |
| _version_ | 1818478985374334976 |
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| author | Julia TCW Claudia M.B. Carvalho Bo Yuan Shen Gu Alyssa N. Altheimer Shane McCarthy Dheeraj Malhotra Jonathan Sebat Arthur J. Siegel Uwe Rudolph James R. Lupski Deborah L. Levy Kristen J. Brennand |
| author_facet | Julia TCW Claudia M.B. Carvalho Bo Yuan Shen Gu Alyssa N. Altheimer Shane McCarthy Dheeraj Malhotra Jonathan Sebat Arthur J. Siegel Uwe Rudolph James R. Lupski Deborah L. Levy Kristen J. Brennand |
| author_sort | Julia TCW |
| collection | DOAJ |
| description | In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures. |
| first_indexed | 2024-12-10T09:55:07Z |
| format | Article |
| id | doaj.art-5444bb3114534651822ec6346e405cd0 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-10T09:55:07Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-5444bb3114534651822ec6346e405cd02022-12-22T01:53:32ZengElsevierStem Cell Reports2213-67112017-03-018351952810.1016/j.stemcr.2017.01.010Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of PsychosisJulia TCW0Claudia M.B. Carvalho1Bo Yuan2Shen Gu3Alyssa N. Altheimer4Shane McCarthy5Dheeraj Malhotra6Jonathan Sebat7Arthur J. Siegel8Uwe Rudolph9James R. Lupski10Deborah L. Levy11Kristen J. Brennand12Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USADepartment of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USADepartment of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USADepartment of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USADepartments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USACold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USAF. Hoffmann-La Roche Ltd, 4070 Basel, SwitzerlandDepartments of Psychiatry, Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USADepartment of Internal Medicine, McLean Hospital, Belmont, MA 02478, USALaboratory of Genetic Neuropharmacology, McLean Hospital, Belmont, MA 02478, USADepartment of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USADepartment of Psychiatry, Harvard Medical School, Boston, MA 02115, USADepartments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USAIn the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.http://www.sciencedirect.com/science/article/pii/S22136711173002559p24.1marker chromosomehuman induced pluripotent stem cellneural progenitor cellschizophreniabipolar disorderisogenicmosaic |
| spellingShingle | Julia TCW Claudia M.B. Carvalho Bo Yuan Shen Gu Alyssa N. Altheimer Shane McCarthy Dheeraj Malhotra Jonathan Sebat Arthur J. Siegel Uwe Rudolph James R. Lupski Deborah L. Levy Kristen J. Brennand Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis Stem Cell Reports 9p24.1 marker chromosome human induced pluripotent stem cell neural progenitor cell schizophrenia bipolar disorder isogenic mosaic |
| title | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis |
| title_full | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis |
| title_fullStr | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis |
| title_full_unstemmed | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis |
| title_short | Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis |
| title_sort | divergent levels of marker chromosomes in an hipsc based model of psychosis |
| topic | 9p24.1 marker chromosome human induced pluripotent stem cell neural progenitor cell schizophrenia bipolar disorder isogenic mosaic |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117300255 |
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