Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene
Cucumber mosaic virus possesses 2b gene known as a suppressor of post-transcriptional gene silencing (PTGS). To investigate its function and effect in plant, transgenic Nicotiana benethamiana expressing 2b gene was developed and analyzed in phenotypic characteristics and differential gene expression...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2015-09-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?uid=1452&&vmd=Full |
| _version_ | 1818567185283416064 |
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| author | Seong-Han Sohn Hong-Soo Choi Jung-Sun Kim So-Yoon Won Do-Sun Kim Yul-Kyun Ahn Yoon-Hee Kim |
| author_facet | Seong-Han Sohn Hong-Soo Choi Jung-Sun Kim So-Yoon Won Do-Sun Kim Yul-Kyun Ahn Yoon-Hee Kim |
| author_sort | Seong-Han Sohn |
| collection | DOAJ |
| description | Cucumber mosaic virus possesses 2b gene known as a suppressor of post-transcriptional gene silencing (PTGS). To investigate its function and effect in plant, transgenic Nicotiana benethamiana expressing 2b gene was developed and analyzed in phenotypic characteristics and differential gene expression (DEG) comparing with wild-type. Eight lines of transgenic plants (T0) were obtained with difficulty and showed severe deformed phenotypes in leaves, flowers, petioles and etc. Moreover, transgenic plants were hardly able to set seeds, but small amounts of seeds were barely produced in some of transgene-hemizygous plants. DEG analysis showed that transgenic plant ectopically accumulated diverse RNA transcripts at higher levels than wild-type probably due to the disturbance in RNA metabolism, especially of RNA decay, caused by 2b-mediated inhibition of PTGS. These ectopic accumulations of RNAs disrupt protein and RNA homeostasis and then subsequently lead to abnormal phenotypes of transgenic plants. |
| first_indexed | 2024-12-14T06:20:09Z |
| format | Article |
| id | doaj.art-54465ffb38574be4b70e5ddfe84eb642 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-12-14T06:20:09Z |
| publishDate | 2015-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-54465ffb38574be4b70e5ddfe84eb6422022-12-21T23:13:52ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912015-09-01213186192http://dx.doi.org/10.5423/RPD.2015.21.3.186Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b geneSeong-Han Sohn0Hong-Soo Choi1 Jung-Sun Kim2So-Yoon Won3Do-Sun Kim4Yul-Kyun Ahn5 Yoon-Hee Kim6Rural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaRural Development Administration, Wanju 565-852, KoreaCucumber mosaic virus possesses 2b gene known as a suppressor of post-transcriptional gene silencing (PTGS). To investigate its function and effect in plant, transgenic Nicotiana benethamiana expressing 2b gene was developed and analyzed in phenotypic characteristics and differential gene expression (DEG) comparing with wild-type. Eight lines of transgenic plants (T0) were obtained with difficulty and showed severe deformed phenotypes in leaves, flowers, petioles and etc. Moreover, transgenic plants were hardly able to set seeds, but small amounts of seeds were barely produced in some of transgene-hemizygous plants. DEG analysis showed that transgenic plant ectopically accumulated diverse RNA transcripts at higher levels than wild-type probably due to the disturbance in RNA metabolism, especially of RNA decay, caused by 2b-mediated inhibition of PTGS. These ectopic accumulations of RNAs disrupt protein and RNA homeostasis and then subsequently lead to abnormal phenotypes of transgenic plants.http://www.online-rpd.org/journal/view.html?uid=1452&&vmd=FullCucumber mosaic virus (CMV)Differential gene expression (DEG)Nicotiana benthamianaPost-transcriptional gene silencing (PTGS) |
| spellingShingle | Seong-Han Sohn Hong-Soo Choi Jung-Sun Kim So-Yoon Won Do-Sun Kim Yul-Kyun Ahn Yoon-Hee Kim Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene Research in Plant Disease Cucumber mosaic virus (CMV) Differential gene expression (DEG) Nicotiana benthamiana Post-transcriptional gene silencing (PTGS) |
| title | Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene |
| title_full | Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene |
| title_fullStr | Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene |
| title_full_unstemmed | Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene |
| title_short | Phenotypic and Transcriptomic Analysis of Nicotiana benthamiana Expressing Cucumber mosaic virus 2b gene |
| title_sort | phenotypic and transcriptomic analysis of nicotiana benthamiana expressing cucumber mosaic virus 2b gene |
| topic | Cucumber mosaic virus (CMV) Differential gene expression (DEG) Nicotiana benthamiana Post-transcriptional gene silencing (PTGS) |
| url | http://www.online-rpd.org/journal/view.html?uid=1452&&vmd=Full |
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