Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines
Some hormone-independent breast cancers lack functional estrogen receptors (ERs) and show evidence of a more aggressive metastatic phenotype. A protective role of the ER has also been suggested in hormoneresistant breast cancer progression. In this study, we have investigated the effect of the ectop...
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| Format: | Article |
| Language: | English |
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Elsevier
2007-02-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558607800418 |
| _version_ | 1819199912790720512 |
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| author | Abhik Bandyopadhyay Long Wang Shiau Hui Chin Lu-Zhe Sun |
| author_facet | Abhik Bandyopadhyay Long Wang Shiau Hui Chin Lu-Zhe Sun |
| author_sort | Abhik Bandyopadhyay |
| collection | DOAJ |
| description | Some hormone-independent breast cancers lack functional estrogen receptors (ERs) and show evidence of a more aggressive metastatic phenotype. A protective role of the ER has also been suggested in hormoneresistant breast cancer progression. In this study, we have investigated the effect of the ectopic expression of human ERα on the bone-metastatic potential of highly metastatic ERα-negative human breast cancer MDA-MB-231 and MDA-MB-435-F-L cell lines in an experimental model of bone metastasis in nude mice. ERα overexpression had no effect on the growth of both cell lines but reduced the expression of integrin aVα3 and the receptor activator of NF-κB, which are known to promote bone metastasis. A significant reduction in the incidence of osteolytic bone metastasis was observed by X-ray imaging of the legs and arms of mice inoculated with ERα-expressing clones of MDA-MB-231 cells in comparison to controls. Ectopic expression of ERα in MDA-MB-435-F-L cells also reduced their widespread skeletal metastasis to the legs, arms, spine, and mandible, as detected by whole-mouse enhanced green fluorescent protein imaging. Our study indicates for the first time that stable reintroduction of functional ERα in ERa-negative human breast cancer cells can inhibit their aggressive bone-metastatic potential in an experimental bone metastasis model. |
| first_indexed | 2024-12-23T03:23:53Z |
| format | Article |
| id | doaj.art-54469757504b41d2a8127c5a0d24c2e3 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-23T03:23:53Z |
| publishDate | 2007-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-54469757504b41d2a8127c5a0d24c2e32022-12-21T18:01:54ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022007-02-019211311810.1593/neo.06784Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell LinesAbhik Bandyopadhyay0Long Wang1Shiau Hui Chin2Lu-Zhe Sun3Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229, USADepartment of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229, USADepartment of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229, USASan Antonio Cancer Institute, University of Texas Health Science Center, San Antonio, TX 78229, USASome hormone-independent breast cancers lack functional estrogen receptors (ERs) and show evidence of a more aggressive metastatic phenotype. A protective role of the ER has also been suggested in hormoneresistant breast cancer progression. In this study, we have investigated the effect of the ectopic expression of human ERα on the bone-metastatic potential of highly metastatic ERα-negative human breast cancer MDA-MB-231 and MDA-MB-435-F-L cell lines in an experimental model of bone metastasis in nude mice. ERα overexpression had no effect on the growth of both cell lines but reduced the expression of integrin aVα3 and the receptor activator of NF-κB, which are known to promote bone metastasis. A significant reduction in the incidence of osteolytic bone metastasis was observed by X-ray imaging of the legs and arms of mice inoculated with ERα-expressing clones of MDA-MB-231 cells in comparison to controls. Ectopic expression of ERα in MDA-MB-435-F-L cells also reduced their widespread skeletal metastasis to the legs, arms, spine, and mandible, as detected by whole-mouse enhanced green fluorescent protein imaging. Our study indicates for the first time that stable reintroduction of functional ERα in ERa-negative human breast cancer cells can inhibit their aggressive bone-metastatic potential in an experimental bone metastasis model.http://www.sciencedirect.com/science/article/pii/S1476558607800418Skeletal metastasisestrogen receptorbreast cancerwholemouse imagingmetastasis-related genes |
| spellingShingle | Abhik Bandyopadhyay Long Wang Shiau Hui Chin Lu-Zhe Sun Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines Neoplasia: An International Journal for Oncology Research Skeletal metastasis estrogen receptor breast cancer wholemouse imaging metastasis-related genes |
| title | Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines |
| title_full | Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines |
| title_fullStr | Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines |
| title_full_unstemmed | Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines |
| title_short | Inhibition of Skeletal Metastasis by Ectopic ERα Expression in ERα-Negative Human Breast Cancer Cell Lines |
| title_sort | inhibition of skeletal metastasis by ectopic erα expression in erα negative human breast cancer cell lines |
| topic | Skeletal metastasis estrogen receptor breast cancer wholemouse imaging metastasis-related genes |
| url | http://www.sciencedirect.com/science/article/pii/S1476558607800418 |
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