Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats
Background: This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats. Materials and Methods:...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Saudi Journal of Biological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X23002826 |
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| author | Maria Amir Nurhusien Yimer Mark Hiew Sabri Mohd Yusoff Bedru Hussen Abdul Quddus |
| author_facet | Maria Amir Nurhusien Yimer Mark Hiew Sabri Mohd Yusoff Bedru Hussen Abdul Quddus |
| author_sort | Maria Amir |
| collection | DOAJ |
| description | Background: This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats. Materials and Methods: The experiments were performed on 24 (6 × 4 groups) adult female SD rats aged 12 weeks old. G1 was the control group and received a normal diet with normal saline. However, on pregnancy days 3 (Pd3) and 4 (Pd4), G2, G3, and G4 were given normal saline and subcutaneously administered IMC twice daily at different doses of 4.33, 4.66 and 5.00 mg/kg body weight, respectively. The rats were euthanized on day 8 of pregnancy (Pd8). The uterus was excised and examined for signs of pregnancy, followed by tissue samples from liver, kidney, and ovary (for histomorphological examination using haematoxylin and eosin stain). Results: All IMC treatment doses disrupted the implantation process and caused a significant reduction in embryo development. Analysis for histopathological changes revealed that IMC doses above 4.33 mg/kg body weight caused more adverse reproductive health effects in rats. Vasoconstriction and micro vascularization were detected in the liver, while degenerative Bowman's capsules and inflammatory cells were observed in kidney sections from IMC-treated rats. Conclusion: IMC therapy interfered with implantation and embryo development in rats, resulting in significant uterine vasoconstriction and atrophy, 4.33 mg/kg bwt dose appeared to be optimum to establish embryo implantation dysfunction in SD rats. |
| first_indexed | 2024-03-08T21:50:52Z |
| format | Article |
| id | doaj.art-5446d9b828f8490f95655e1d2434a356 |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-03-08T21:50:52Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-5446d9b828f8490f95655e1d2434a3562023-12-20T07:33:39ZengElsevierSaudi Journal of Biological Sciences1319-562X2023-12-013012103837Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of ratsMaria Amir0Nurhusien Yimer1Mark Hiew2Sabri Mohd Yusoff3Bedru Hussen4Abdul Quddus5Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Faculty of Veterinary and Animal Sciences, Department of Veterinary Physiology and Biochemistry, Ziauddin University, 75600 Karachi, PakistanDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, East Java, Indonesia; Corresponding author.Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaSchool of Public Health, University of Hong Kong, 7 Sassoon Rd, Sandy Bay, Hong KongFaculty of Veterinary and Animal Science, Lasbela University of Agriculture Water and Marine Science, 90150 Uthal, Balochistan, PakistanBackground: This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats. Materials and Methods: The experiments were performed on 24 (6 × 4 groups) adult female SD rats aged 12 weeks old. G1 was the control group and received a normal diet with normal saline. However, on pregnancy days 3 (Pd3) and 4 (Pd4), G2, G3, and G4 were given normal saline and subcutaneously administered IMC twice daily at different doses of 4.33, 4.66 and 5.00 mg/kg body weight, respectively. The rats were euthanized on day 8 of pregnancy (Pd8). The uterus was excised and examined for signs of pregnancy, followed by tissue samples from liver, kidney, and ovary (for histomorphological examination using haematoxylin and eosin stain). Results: All IMC treatment doses disrupted the implantation process and caused a significant reduction in embryo development. Analysis for histopathological changes revealed that IMC doses above 4.33 mg/kg body weight caused more adverse reproductive health effects in rats. Vasoconstriction and micro vascularization were detected in the liver, while degenerative Bowman's capsules and inflammatory cells were observed in kidney sections from IMC-treated rats. Conclusion: IMC therapy interfered with implantation and embryo development in rats, resulting in significant uterine vasoconstriction and atrophy, 4.33 mg/kg bwt dose appeared to be optimum to establish embryo implantation dysfunction in SD rats.http://www.sciencedirect.com/science/article/pii/S1319562X23002826Embryo implantation dysfunctionHistopathologyIndomethacinSD ratsVital organs |
| spellingShingle | Maria Amir Nurhusien Yimer Mark Hiew Sabri Mohd Yusoff Bedru Hussen Abdul Quddus Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats Saudi Journal of Biological Sciences Embryo implantation dysfunction Histopathology Indomethacin SD rats Vital organs |
| title | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_full | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_fullStr | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_full_unstemmed | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_short | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_sort | effect of indomethacin on embryo implantation and histomorphology of uterus ovary kidney and liver of rats |
| topic | Embryo implantation dysfunction Histopathology Indomethacin SD rats Vital organs |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X23002826 |
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