Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE)
Abstract Background The pristane-induced lupus (PIL) model is a useful tool for studying environmental-related systemic lupus erythematosus (SLE). However, neuropsychiatric manifestations in this model have not been investigated in detail. Because neuropsychiatric lupus (NPSLE) is an important compl...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Behavioral and Brain Functions |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12993-023-00205-y |
| _version_ | 1811165771582144512 |
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| author | Yang Yun Xuejiao Wang Jingyi Xu Chenye Jin Jingyu Chen Xueru Wang Jianing Wang Ling Qin Pingting Yang |
| author_facet | Yang Yun Xuejiao Wang Jingyi Xu Chenye Jin Jingyu Chen Xueru Wang Jianing Wang Ling Qin Pingting Yang |
| author_sort | Yang Yun |
| collection | DOAJ |
| description | Abstract Background The pristane-induced lupus (PIL) model is a useful tool for studying environmental-related systemic lupus erythematosus (SLE). However, neuropsychiatric manifestations in this model have not been investigated in detail. Because neuropsychiatric lupus (NPSLE) is an important complication of SLE, we investigated the neuropsychiatric symptoms in the PIL mouse model to evaluate its suitability for NPSLE studies. Results PIL mice showed olfactory dysfunction accompanied by an anxiety- and depression-like phenotype at month 2 or 4 after pristane injection. The levels of cytokines (IL-1β, IFN-α, IFN-β, IL-10, IFN-γ, IL-6, TNF-α and IL-17A) and chemokines (CCL2 and CXCL10) in the brain and blood–brain barrier (BBB) permeability increased significantly from week 2 or month 1, and persisted throughout the observed course of the disease. Notably, IgG deposition in the choroid plexus and lateral ventricle wall were observed at month 1 and both astrocytes and microglia were activated. Persistent activation of astrocytes was detected throughout the observed course of the disease, while microglial activation diminished dramatically at month 4. Lipofuscin deposition, a sign of neuronal damage, was detected in cortical and hippocampal neurons from month 4 to 8. Conclusion PIL mice exhibit a series of characteristic behavioral deficits and pathological changes in the brain, and therefore might be suitable for investigating disease pathogenesis and for evaluating potential therapeutic targets for environmental-related NPSLE. |
| first_indexed | 2024-04-10T15:42:45Z |
| format | Article |
| id | doaj.art-5447f7fa0c024fbab687f379811e6453 |
| institution | Directory Open Access Journal |
| issn | 1744-9081 |
| language | English |
| last_indexed | 2024-04-10T15:42:45Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Behavioral and Brain Functions |
| spelling | doaj.art-5447f7fa0c024fbab687f379811e64532023-02-12T12:18:20ZengBMCBehavioral and Brain Functions1744-90812023-02-0119111910.1186/s12993-023-00205-yPristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE)Yang Yun0Xuejiao Wang1Jingyi Xu2Chenye Jin3Jingyu Chen4Xueru Wang5Jianing Wang6Ling Qin7Pingting Yang8Department of Nephrology, Shengjing Hospital of China Medical UniversityDepartment of Physiology, China Medical UniversityDepartment of Rheumatology and Immunology, First Affiliated Hospital, China Medical UniversityDepartment of Rheumatology and Immunology, First Affiliated Hospital, China Medical UniversityDepartment of Physiology, China Medical UniversityDepartment of Physiology, China Medical UniversityDepartment of Rheumatology and Immunology, First Affiliated Hospital, China Medical UniversityDepartment of Physiology, China Medical UniversityDepartment of Rheumatology and Immunology, First Affiliated Hospital, China Medical UniversityAbstract Background The pristane-induced lupus (PIL) model is a useful tool for studying environmental-related systemic lupus erythematosus (SLE). However, neuropsychiatric manifestations in this model have not been investigated in detail. Because neuropsychiatric lupus (NPSLE) is an important complication of SLE, we investigated the neuropsychiatric symptoms in the PIL mouse model to evaluate its suitability for NPSLE studies. Results PIL mice showed olfactory dysfunction accompanied by an anxiety- and depression-like phenotype at month 2 or 4 after pristane injection. The levels of cytokines (IL-1β, IFN-α, IFN-β, IL-10, IFN-γ, IL-6, TNF-α and IL-17A) and chemokines (CCL2 and CXCL10) in the brain and blood–brain barrier (BBB) permeability increased significantly from week 2 or month 1, and persisted throughout the observed course of the disease. Notably, IgG deposition in the choroid plexus and lateral ventricle wall were observed at month 1 and both astrocytes and microglia were activated. Persistent activation of astrocytes was detected throughout the observed course of the disease, while microglial activation diminished dramatically at month 4. Lipofuscin deposition, a sign of neuronal damage, was detected in cortical and hippocampal neurons from month 4 to 8. Conclusion PIL mice exhibit a series of characteristic behavioral deficits and pathological changes in the brain, and therefore might be suitable for investigating disease pathogenesis and for evaluating potential therapeutic targets for environmental-related NPSLE.https://doi.org/10.1186/s12993-023-00205-yNeuropsychiatric lupusMouse modelBehavioral deficitCytokineIgGGlia cells |
| spellingShingle | Yang Yun Xuejiao Wang Jingyi Xu Chenye Jin Jingyu Chen Xueru Wang Jianing Wang Ling Qin Pingting Yang Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) Behavioral and Brain Functions Neuropsychiatric lupus Mouse model Behavioral deficit Cytokine IgG Glia cells |
| title | Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) |
| title_full | Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) |
| title_fullStr | Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) |
| title_full_unstemmed | Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) |
| title_short | Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE) |
| title_sort | pristane induced lupus mice as a model for neuropsychiatric lupus npsle |
| topic | Neuropsychiatric lupus Mouse model Behavioral deficit Cytokine IgG Glia cells |
| url | https://doi.org/10.1186/s12993-023-00205-y |
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