Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation
The induction of defense responses in <i>Pinus sylvestris</i> L. seedlings by methyl jasmonate (MeJA) was investigated in three experiments. Two different MeJA application methods were tested, and induction of defense responses was assayed by seedling inoculation with <i>Heterobasi...
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MDPI AG
2021-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/5/628 |
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| author | Ilze Šņepste Baiba Krivmane Vilnis Šķipars Astra Zaluma Dainis E. Ruņģis |
| author_facet | Ilze Šņepste Baiba Krivmane Vilnis Šķipars Astra Zaluma Dainis E. Ruņģis |
| author_sort | Ilze Šņepste |
| collection | DOAJ |
| description | The induction of defense responses in <i>Pinus sylvestris</i> L. seedlings by methyl jasmonate (MeJA) was investigated in three experiments. Two different MeJA application methods were tested, and induction of defense responses was assayed by seedling inoculation with <i>Heterobasidion annosum</i> (Fr.) Bref. and <i>Lophodermium seditiosum</i> Minter, Staley and Millar. In the first experiment, five-year-old <i>P. sylvestris</i> ramets of one clone were directly treated with MeJA, followed by inoculation with <i>H. annosum</i>. In the second experiment, open-pollinated Scots pine seedlings were treated with MeJA by direct spraying and vaporization, and inoculation with <i>H. annosum</i> was done using a slightly modified protocol. In the third experiment, open-pollinated Scots pine seedlings were treated with MeJA by vaporization and inoculated with <i>L. seditiosum</i>. Direct application of MeJA induced seedling mortality, and in some cases, decreased resistance to inoculation with <i>H. annosum</i>. Application of MeJA by vaporization was less stressful for seedlings, and resulted in increased resistance to both <i>H. annosum</i> and <i>L. seditiosum</i>. In addition, an unforeseen <i>Neodiprion sertifer</i> (Geoffroy) and <i>Hylobius abietis</i> L. infestation provided anecdotal evidence of the efficacy of MeJA in inducing resistance to insect pests as well. Further studies are required on the induction of resistance to additional diseases and pests. Induced resistance could be used as a possible protective mechanism for Scots pine seedlings prior to planting during reforestation of stands to increase vitality and survival. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T11:23:50Z |
| publishDate | 2021-05-01 |
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| series | Forests |
| spelling | doaj.art-5449b0dea3814446985e389b08ce8b8f2023-11-21T19:48:10ZengMDPI AGForests1999-49072021-05-0112562810.3390/f12050628Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> InoculationIlze Šņepste0Baiba Krivmane1Vilnis Šķipars2Astra Zaluma3Dainis E. Ruņģis4Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rīgas Street, LV-2169 Salaspils, LatviaGenetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rīgas Street, LV-2169 Salaspils, LatviaGenetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rīgas Street, LV-2169 Salaspils, LatviaForest Phytopathology and Mycology, Latvian State Forest Research Institute “Silava”, 111 Rīgas Street, LV-2169 Salaspils, LatviaGenetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rīgas Street, LV-2169 Salaspils, LatviaThe induction of defense responses in <i>Pinus sylvestris</i> L. seedlings by methyl jasmonate (MeJA) was investigated in three experiments. Two different MeJA application methods were tested, and induction of defense responses was assayed by seedling inoculation with <i>Heterobasidion annosum</i> (Fr.) Bref. and <i>Lophodermium seditiosum</i> Minter, Staley and Millar. In the first experiment, five-year-old <i>P. sylvestris</i> ramets of one clone were directly treated with MeJA, followed by inoculation with <i>H. annosum</i>. In the second experiment, open-pollinated Scots pine seedlings were treated with MeJA by direct spraying and vaporization, and inoculation with <i>H. annosum</i> was done using a slightly modified protocol. In the third experiment, open-pollinated Scots pine seedlings were treated with MeJA by vaporization and inoculated with <i>L. seditiosum</i>. Direct application of MeJA induced seedling mortality, and in some cases, decreased resistance to inoculation with <i>H. annosum</i>. Application of MeJA by vaporization was less stressful for seedlings, and resulted in increased resistance to both <i>H. annosum</i> and <i>L. seditiosum</i>. In addition, an unforeseen <i>Neodiprion sertifer</i> (Geoffroy) and <i>Hylobius abietis</i> L. infestation provided anecdotal evidence of the efficacy of MeJA in inducing resistance to insect pests as well. Further studies are required on the induction of resistance to additional diseases and pests. Induced resistance could be used as a possible protective mechanism for Scots pine seedlings prior to planting during reforestation of stands to increase vitality and survival.https://www.mdpi.com/1999-4907/12/5/628thaumatin-like proteingene expressioninduced resistancefungal pathogens |
| spellingShingle | Ilze Šņepste Baiba Krivmane Vilnis Šķipars Astra Zaluma Dainis E. Ruņģis Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation Forests thaumatin-like protein gene expression induced resistance fungal pathogens |
| title | Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation |
| title_full | Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation |
| title_fullStr | Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation |
| title_full_unstemmed | Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation |
| title_short | Induction of Defense Responses in <i>Pinus sylvestris</i> Seedlings by Methyl Jasmonate and Response to <i>Heterobasidion annosum</i> and <i>Lophodermium seditiosum</i> Inoculation |
| title_sort | induction of defense responses in i pinus sylvestris i seedlings by methyl jasmonate and response to i heterobasidion annosum i and i lophodermium seditiosum i inoculation |
| topic | thaumatin-like protein gene expression induced resistance fungal pathogens |
| url | https://www.mdpi.com/1999-4907/12/5/628 |
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