Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad
Abstract Background Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad. Methods In total, 200 fresh stool samples were collected from...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13756-019-0626-z |
| _version_ | 1819203844813357056 |
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| author | Oumar Ouchar Mahamat Abdelsalam Tidjani Manon Lounnas Mallorie Hide Julio Benavides Calèbe Somasse Abdoul-Salam Ouedraogo Soufiane Sanou Christian Carrière Anne-Laure Bañuls Hélène Jean-Pierre Yann Dumont Sylvain Godreuil |
| author_facet | Oumar Ouchar Mahamat Abdelsalam Tidjani Manon Lounnas Mallorie Hide Julio Benavides Calèbe Somasse Abdoul-Salam Ouedraogo Soufiane Sanou Christian Carrière Anne-Laure Bañuls Hélène Jean-Pierre Yann Dumont Sylvain Godreuil |
| author_sort | Oumar Ouchar Mahamat |
| collection | DOAJ |
| description | Abstract Background Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad. Methods In total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method. Results ESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored bla CTX-M genes: bla CTX-M-15 in 94.25% (82/87) and bla CTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains. Conclusions The high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria. |
| first_indexed | 2024-12-23T04:26:23Z |
| format | Article |
| id | doaj.art-544ac4448fe04cff9c5ec29c802bc2a2 |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-23T04:26:23Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-544ac4448fe04cff9c5ec29c802bc2a22022-12-21T18:00:08ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-10-01811710.1186/s13756-019-0626-zFecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in ChadOumar Ouchar Mahamat0Abdelsalam Tidjani1Manon Lounnas2Mallorie Hide3Julio Benavides4Calèbe Somasse5Abdoul-Salam Ouedraogo6Soufiane Sanou7Christian Carrière8Anne-Laure Bañuls9Hélène Jean-Pierre10Yann Dumont11Sylvain Godreuil12Laboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierFaculté de Médecine, Université de N’DjaménaLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierMIVEGEC, IRD, CNRS, Université de Montpellier7 Departamento de Ecología y Biodiversidad, Facultad de Ciencias de la Vida, Universidad Andrés BelloLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierService de bactériologie-virologie, Département des laboratoires, Centre Hospitalier Universitaire Souro SanouService de bactériologie-virologie, Département des laboratoires, Centre Hospitalier Universitaire Souro SanouLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierMIVEGEC, IRD, CNRS, Université de MontpellierLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierLaboratoire de Bactériologie, Centre Hospitalier Universitaire de MontpellierAbstract Background Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad. Methods In total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method. Results ESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored bla CTX-M genes: bla CTX-M-15 in 94.25% (82/87) and bla CTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains. Conclusions The high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria.http://link.springer.com/article/10.1186/s13756-019-0626-zESBLEnterobacteriaceaeFecal carriageChad |
| spellingShingle | Oumar Ouchar Mahamat Abdelsalam Tidjani Manon Lounnas Mallorie Hide Julio Benavides Calèbe Somasse Abdoul-Salam Ouedraogo Soufiane Sanou Christian Carrière Anne-Laure Bañuls Hélène Jean-Pierre Yann Dumont Sylvain Godreuil Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad Antimicrobial Resistance and Infection Control ESBL Enterobacteriaceae Fecal carriage Chad |
| title | Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad |
| title_full | Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad |
| title_fullStr | Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad |
| title_full_unstemmed | Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad |
| title_short | Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad |
| title_sort | fecal carriage of extended spectrum β lactamase producing enterobacteriaceae in hospital and community settings in chad |
| topic | ESBL Enterobacteriaceae Fecal carriage Chad |
| url | http://link.springer.com/article/10.1186/s13756-019-0626-z |
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