An Immersible Microgripper for Pancreatic Islet and Organoid Research
To improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregate...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/2/67 |
| _version_ | 1827656963406692352 |
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| author | Eike Früh Sebastian Bütefisch Benjamin Gursky Dennis Brüning Monika Leester-Schädel Andreas Dietzel Ingo Rustenbeck |
| author_facet | Eike Früh Sebastian Bütefisch Benjamin Gursky Dennis Brüning Monika Leester-Schädel Andreas Dietzel Ingo Rustenbeck |
| author_sort | Eike Früh |
| collection | DOAJ |
| description | To improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregates and organoids in a well-controlled manner, without causing structural damage or disturbing the function of interest, new instrumentation is needed. In particular, the precise and stable positioning in a cell bath with flow rates sufficient to characterize the kinetic responses to physiological or pharmacological stimuli can be a demanding task. Here, we present data that demonstrate that microgrippers are well suited to this task. The current version is able to work in aqueous solutions and was shown to position isolated pancreatic islets and 3D aggregates of insulin-secreting MIN6-cells. A stable hold required a gripping force of less than 30 μN and did not affect the cellular integrity. It was maintained even with high flow rates of the bath perfusion, and it was precise enough to permit the simultaneous microfluorimetric measurements and membrane potential measurements of the single cells within the islet through the use of patch-clamp electrodes. |
| first_indexed | 2024-03-09T22:35:40Z |
| format | Article |
| id | doaj.art-544e98b459e046e6aa8ab81d97ae4e15 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T22:35:40Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-544e98b459e046e6aa8ab81d97ae4e152023-11-23T18:48:35ZengMDPI AGBioengineering2306-53542022-02-01926710.3390/bioengineering9020067An Immersible Microgripper for Pancreatic Islet and Organoid ResearchEike Früh0Sebastian Bütefisch1Benjamin Gursky2Dennis Brüning3Monika Leester-Schädel4Andreas Dietzel5Ingo Rustenbeck6Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, GermanyPTB–Physikalisch-Technische Bundesanstalt/Federal Institute of Metrology, D38116 Braunschweig, GermanyPVZ-Center of Pharmaceutical Engineering of the Technische Universität Braunschweig, Technische Universität Braunschweig, D38106 Braunschweig, GermanyInstitute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, GermanyPVZ-Center of Pharmaceutical Engineering of the Technische Universität Braunschweig, Technische Universität Braunschweig, D38106 Braunschweig, GermanyPVZ-Center of Pharmaceutical Engineering of the Technische Universität Braunschweig, Technische Universität Braunschweig, D38106 Braunschweig, GermanyInstitute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, GermanyTo improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregates and organoids in a well-controlled manner, without causing structural damage or disturbing the function of interest, new instrumentation is needed. In particular, the precise and stable positioning in a cell bath with flow rates sufficient to characterize the kinetic responses to physiological or pharmacological stimuli can be a demanding task. Here, we present data that demonstrate that microgrippers are well suited to this task. The current version is able to work in aqueous solutions and was shown to position isolated pancreatic islets and 3D aggregates of insulin-secreting MIN6-cells. A stable hold required a gripping force of less than 30 μN and did not affect the cellular integrity. It was maintained even with high flow rates of the bath perfusion, and it was precise enough to permit the simultaneous microfluorimetric measurements and membrane potential measurements of the single cells within the islet through the use of patch-clamp electrodes.https://www.mdpi.com/2306-5354/9/2/67cytosolic calciumelectrophysiologypancreatic isletmicrogripperorganoidsSU-8 |
| spellingShingle | Eike Früh Sebastian Bütefisch Benjamin Gursky Dennis Brüning Monika Leester-Schädel Andreas Dietzel Ingo Rustenbeck An Immersible Microgripper for Pancreatic Islet and Organoid Research Bioengineering cytosolic calcium electrophysiology pancreatic islet microgripper organoids SU-8 |
| title | An Immersible Microgripper for Pancreatic Islet and Organoid Research |
| title_full | An Immersible Microgripper for Pancreatic Islet and Organoid Research |
| title_fullStr | An Immersible Microgripper for Pancreatic Islet and Organoid Research |
| title_full_unstemmed | An Immersible Microgripper for Pancreatic Islet and Organoid Research |
| title_short | An Immersible Microgripper for Pancreatic Islet and Organoid Research |
| title_sort | immersible microgripper for pancreatic islet and organoid research |
| topic | cytosolic calcium electrophysiology pancreatic islet microgripper organoids SU-8 |
| url | https://www.mdpi.com/2306-5354/9/2/67 |
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