Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study
The rhizome of Acorus calamus, an herb widely used in Indian system of medicine for many ailments including epilepsy, mental illness and rheumatism, was subjected to soxhlet extraction to elucidate antioxidant property of different solvent extracts using in vitro assays. The benzene extract was most...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2016-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016000057 |
| _version_ | 1797700006607585280 |
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| author | M. Devaki R. Nirupama M. Nirupama H.N. Yajurvedi |
| author_facet | M. Devaki R. Nirupama M. Nirupama H.N. Yajurvedi |
| author_sort | M. Devaki |
| collection | DOAJ |
| description | The rhizome of Acorus calamus, an herb widely used in Indian system of medicine for many ailments including epilepsy, mental illness and rheumatism, was subjected to soxhlet extraction to elucidate antioxidant property of different solvent extracts using in vitro assays. The benzene extract was most potent in scavenging hydroxyl and superoxide radicals and in reducing 1,1-diphenyl-2-picryl hydrazyl and ferric reducing antioxidant power. In addition the benzene extract prevented oxidative damage to DNA and mitochondria. It was also effective in preventing stress -induced decrease in total plasma anti-oxidant activity as determined in vivo using rat model wherein stress was induced by exposing to restraint and forced swimming. The minimum effective dose of the benzene extract was 5 mg/kg body weight (oral), and at this dose, its effect was similar to the same dose of a standard anti-oxidant, ascorbic acid. The study for the first time, clearly demonstrates a potent anti-oxidant activity of A. calamus combining in vitro and in vivo results. Hence, the therapeutic value of this herb maybe due to its anti-oxidant property. |
| first_indexed | 2024-03-12T04:16:58Z |
| format | Article |
| id | doaj.art-545b258816d44dfb881c9a46347cc094 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T04:16:58Z |
| publishDate | 2016-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-545b258816d44dfb881c9a46347cc0942023-09-03T10:35:44ZengTsinghua University PressFood Science and Human Wellness2213-45302016-06-0152768410.1016/j.fshw.2016.02.003Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro studyM. Devaki0R. Nirupama1M. Nirupama2H.N. Yajurvedi3Department of Zoology, University of Mysore, Manasagangotri, Mysore 570 006, IndiaDepartment of Zoology, University of Mysore, Manasagangotri, Mysore 570 006, IndiaDepartment of Zoology, University of Mysore, Manasagangotri, Mysore 570 006, IndiaDepartment of Zoology, University of Mysore, Manasagangotri, Mysore 570 006, IndiaThe rhizome of Acorus calamus, an herb widely used in Indian system of medicine for many ailments including epilepsy, mental illness and rheumatism, was subjected to soxhlet extraction to elucidate antioxidant property of different solvent extracts using in vitro assays. The benzene extract was most potent in scavenging hydroxyl and superoxide radicals and in reducing 1,1-diphenyl-2-picryl hydrazyl and ferric reducing antioxidant power. In addition the benzene extract prevented oxidative damage to DNA and mitochondria. It was also effective in preventing stress -induced decrease in total plasma anti-oxidant activity as determined in vivo using rat model wherein stress was induced by exposing to restraint and forced swimming. The minimum effective dose of the benzene extract was 5 mg/kg body weight (oral), and at this dose, its effect was similar to the same dose of a standard anti-oxidant, ascorbic acid. The study for the first time, clearly demonstrates a potent anti-oxidant activity of A. calamus combining in vitro and in vivo results. Hence, the therapeutic value of this herb maybe due to its anti-oxidant property.http://www.sciencedirect.com/science/article/pii/S2213453016000057Acorus calamusAntioxidantForced swimmingRadical scavengingDNA protection assay |
| spellingShingle | M. Devaki R. Nirupama M. Nirupama H.N. Yajurvedi Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study Food Science and Human Wellness Acorus calamus Antioxidant Forced swimming Radical scavenging DNA protection assay |
| title | Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study |
| title_full | Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study |
| title_fullStr | Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study |
| title_full_unstemmed | Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study |
| title_short | Protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: An in vivo and in vitro study |
| title_sort | protective effect of rhizome extracts of the herb vacha acorus calamus against oxidative damage an in vivo and in vitro study |
| topic | Acorus calamus Antioxidant Forced swimming Radical scavenging DNA protection assay |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016000057 |
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