ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner
Emerging evidence suggests that chromodomain-helicase-DNA-binding (CHD) proteins are involved in stem cell maintenance and differentiation via the coordination of chromatin structure and gene expression. However, the molecular function of some CHD proteins in stem cell regulation is still poorly und...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/9/12/428 |
| _version_ | 1797546524055437312 |
|---|---|
| author | Hyunjin Yoo Hyeonwoo La Eun Joo Lee Hee-Jin Choi Jeongheon Oh Nguyen Xuan Thang Kwonho Hong |
| author_facet | Hyunjin Yoo Hyeonwoo La Eun Joo Lee Hee-Jin Choi Jeongheon Oh Nguyen Xuan Thang Kwonho Hong |
| author_sort | Hyunjin Yoo |
| collection | DOAJ |
| description | Emerging evidence suggests that chromodomain-helicase-DNA-binding (CHD) proteins are involved in stem cell maintenance and differentiation via the coordination of chromatin structure and gene expression. However, the molecular function of some CHD proteins in stem cell regulation is still poorly understood. Herein, we show that <i>Chd9</i> knockdown (KD) in mouse embryonic stem cells (ESCs) cultured in normal serum media, not in 2i-leukemia inhibitory factor (LIF) media, causes rapid cell proliferation. This is caused by transcriptional regulation related to the cell cycle and the response to growth factors. Our analysis showed that, unlike the serum cultured-<i>Chd9</i> KD ESCs, the 2i-LIF-cultured-<i>Chd9</i> KO ESCs displayed elevated levels of critical G1 phase regulators such as p21 and p27. Consistently, the DNA binding sites of CHD9 overlap with some transcription factor DNA motifs that are associated with genes regulating the cell cycle and growth pathways. These transcription factors include the cycle gene homology region (CHR), Arid5a, and LIN54. Collectively, our results provide new insights into CHD9-mediated gene transcription for controlling the cell cycle of ESCs. |
| first_indexed | 2024-03-10T14:31:01Z |
| format | Article |
| id | doaj.art-5460d96efc6e48d2814405bba33ef7ba |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T14:31:01Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-5460d96efc6e48d2814405bba33ef7ba2023-11-20T22:36:37ZengMDPI AGBiology2079-77372020-11-0191242810.3390/biology9120428ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent MannerHyunjin Yoo0Hyeonwoo La1Eun Joo Lee2Hee-Jin Choi3Jeongheon Oh4Nguyen Xuan Thang5Kwonho Hong6Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaDepartment of Stem Cell and Regenerative Biotechnology and Humanized Pig Center (SRC), Konkuk University, Seoul 05029, KoreaEmerging evidence suggests that chromodomain-helicase-DNA-binding (CHD) proteins are involved in stem cell maintenance and differentiation via the coordination of chromatin structure and gene expression. However, the molecular function of some CHD proteins in stem cell regulation is still poorly understood. Herein, we show that <i>Chd9</i> knockdown (KD) in mouse embryonic stem cells (ESCs) cultured in normal serum media, not in 2i-leukemia inhibitory factor (LIF) media, causes rapid cell proliferation. This is caused by transcriptional regulation related to the cell cycle and the response to growth factors. Our analysis showed that, unlike the serum cultured-<i>Chd9</i> KD ESCs, the 2i-LIF-cultured-<i>Chd9</i> KO ESCs displayed elevated levels of critical G1 phase regulators such as p21 and p27. Consistently, the DNA binding sites of CHD9 overlap with some transcription factor DNA motifs that are associated with genes regulating the cell cycle and growth pathways. These transcription factors include the cycle gene homology region (CHR), Arid5a, and LIN54. Collectively, our results provide new insights into CHD9-mediated gene transcription for controlling the cell cycle of ESCs.https://www.mdpi.com/2079-7737/9/12/428CHD9chromatin structureES cellcell cycletranscription |
| spellingShingle | Hyunjin Yoo Hyeonwoo La Eun Joo Lee Hee-Jin Choi Jeongheon Oh Nguyen Xuan Thang Kwonho Hong ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner Biology CHD9 chromatin structure ES cell cell cycle transcription |
| title | ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner |
| title_full | ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner |
| title_fullStr | ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner |
| title_full_unstemmed | ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner |
| title_short | ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic Stem Cells in a Cell Culture Condition-Dependent Manner |
| title_sort | atp dependent chromatin remodeler chd9 controls the proliferation of embryonic stem cells in a cell culture condition dependent manner |
| topic | CHD9 chromatin structure ES cell cell cycle transcription |
| url | https://www.mdpi.com/2079-7737/9/12/428 |
| work_keys_str_mv | AT hyunjinyoo atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT hyeonwoola atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT eunjoolee atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT heejinchoi atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT jeongheonoh atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT nguyenxuanthang atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner AT kwonhohong atpdependentchromatinremodelerchd9controlstheproliferationofembryonicstemcellsinacellcultureconditiondependentmanner |