Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study
Abstract Objectives To investigate sonication as a new tool in microbiological probing of dental infections. Methods Comparison of a standard probing method: intraoperative swab, with sonication, and vortex of the removed tooth, was performed on 20 carious destructed teeth. Illumina high throughput...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02374-0 |
| _version_ | 1811215432058667008 |
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| author | Oliver Wagendorf Peter Menzel Rolf Schwarzer Norbert Neckel Saskia Preissner Max Heiland Susanne Nahles |
| author_facet | Oliver Wagendorf Peter Menzel Rolf Schwarzer Norbert Neckel Saskia Preissner Max Heiland Susanne Nahles |
| author_sort | Oliver Wagendorf |
| collection | DOAJ |
| description | Abstract Objectives To investigate sonication as a new tool in microbiological probing of dental infections. Methods Comparison of a standard probing method: intraoperative swab, with sonication, and vortex of the removed tooth, was performed on 20 carious destructed teeth. Illumina high throughput sequencing of the 16S-rRNA-gene was used for assessing the microbial composition. Antibiotic susceptibility has been assigned based on known resistances of each detected species. Probing procedures were compared using Bland–Altmann-Test, and antibiotic susceptibility using the Friedmann-Test and alpha-adjusted post-hoc-analysis. Results In total, 60 samples were analysed: 20 intraoperative swabs, 20 vortex fluids, and 20 sonication fluids. Sonication fluid yielded the highest number of bacterial sequencing reads in all three procedures. Comparing the operational taxonomic units (OTUs) of the identified bacteria, significantly more OTUs were found in sonication fluid samples. Phylum and order abundances varied between the three procedures. Significantly more Actinomycetales have been found in sonication fluid samples compared to swab samples. The assigned resistance rates for the identified bacteria (1.79–31.23%) showed no differences between the tested probing procedures. The lowest resistance rates were found for amoxicillin + clavulanate (3.95%) and levofloxacin (3.40%), with the highest in amoxicillin (30.21%) and clindamycin (21.88%). Conclusions By using sonication on extracted teeth, it is possible to get a more comprehensive image of the residing microbial flora compared to the standard procedure. If sonication is not available, vortexing is a potential alternative. In immunocompromised patients, especially when actinomycosis is suspected, sonication should be considered for a more detailed microbiological evaluation of the potential disease-causing microbiome. Due to the high rates of antibiotic resistance, a more targeted antibiotic therapy is favourable. Levofloxacin should be considered as a first-line alternative to amoxicillin + clavulanate in patients with an allergy to penicillin. |
| first_indexed | 2024-04-12T06:21:18Z |
| format | Article |
| id | doaj.art-5461bba86c7b4c74b622421c01e6fcfe |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-12T06:21:18Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-5461bba86c7b4c74b622421c01e6fcfe2022-12-22T03:44:17ZengBMCBMC Oral Health1472-68312022-08-0122111110.1186/s12903-022-02374-0Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot studyOliver Wagendorf0Peter Menzel1Rolf Schwarzer2Norbert Neckel3Saskia Preissner4Max Heiland5Susanne Nahles6Department of Oral and Maxillofacial Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of HealthLabor Berlin – Charité Vivantes GmbHLabor Berlin – Charité Vivantes GmbHDepartment of Oral and Maxillofacial Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of HealthDepartment of Oral and Maxillofacial Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of HealthDepartment of Oral and Maxillofacial Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of HealthDepartment of Oral and Maxillofacial Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of HealthAbstract Objectives To investigate sonication as a new tool in microbiological probing of dental infections. Methods Comparison of a standard probing method: intraoperative swab, with sonication, and vortex of the removed tooth, was performed on 20 carious destructed teeth. Illumina high throughput sequencing of the 16S-rRNA-gene was used for assessing the microbial composition. Antibiotic susceptibility has been assigned based on known resistances of each detected species. Probing procedures were compared using Bland–Altmann-Test, and antibiotic susceptibility using the Friedmann-Test and alpha-adjusted post-hoc-analysis. Results In total, 60 samples were analysed: 20 intraoperative swabs, 20 vortex fluids, and 20 sonication fluids. Sonication fluid yielded the highest number of bacterial sequencing reads in all three procedures. Comparing the operational taxonomic units (OTUs) of the identified bacteria, significantly more OTUs were found in sonication fluid samples. Phylum and order abundances varied between the three procedures. Significantly more Actinomycetales have been found in sonication fluid samples compared to swab samples. The assigned resistance rates for the identified bacteria (1.79–31.23%) showed no differences between the tested probing procedures. The lowest resistance rates were found for amoxicillin + clavulanate (3.95%) and levofloxacin (3.40%), with the highest in amoxicillin (30.21%) and clindamycin (21.88%). Conclusions By using sonication on extracted teeth, it is possible to get a more comprehensive image of the residing microbial flora compared to the standard procedure. If sonication is not available, vortexing is a potential alternative. In immunocompromised patients, especially when actinomycosis is suspected, sonication should be considered for a more detailed microbiological evaluation of the potential disease-causing microbiome. Due to the high rates of antibiotic resistance, a more targeted antibiotic therapy is favourable. Levofloxacin should be considered as a first-line alternative to amoxicillin + clavulanate in patients with an allergy to penicillin.https://doi.org/10.1186/s12903-022-02374-0MicrobiomeTooth extractionSonicationAntibiotic resistance |
| spellingShingle | Oliver Wagendorf Peter Menzel Rolf Schwarzer Norbert Neckel Saskia Preissner Max Heiland Susanne Nahles Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study BMC Oral Health Microbiome Tooth extraction Sonication Antibiotic resistance |
| title | Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study |
| title_full | Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study |
| title_fullStr | Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study |
| title_full_unstemmed | Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study |
| title_short | Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study |
| title_sort | sonication versus the conventional method for evaluation of the dental microbiome a prospective pilot study |
| topic | Microbiome Tooth extraction Sonication Antibiotic resistance |
| url | https://doi.org/10.1186/s12903-022-02374-0 |
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