Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon
Abstract Background The neotropical anopheline mosquito Anopheles darlingi is a major malaria vector in the Americas. Studies on mosquito-associated microbiota have shown that symbiotic bacteria play a major role in host biology. Mosquitoes acquire and transmit microorganisms over their life cycle....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-023-05749-6 |
| _version_ | 1797832219583053824 |
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| author | Katherine D. Mosquera Louise K. J. Nilsson Marta Rodrigues de Oliveira Elerson Matos Rocha Osvaldo Marinotti Sebastian Håkansson Wanderli P. Tadei Antonia Queiroz Lima de Souza Olle Terenius |
| author_facet | Katherine D. Mosquera Louise K. J. Nilsson Marta Rodrigues de Oliveira Elerson Matos Rocha Osvaldo Marinotti Sebastian Håkansson Wanderli P. Tadei Antonia Queiroz Lima de Souza Olle Terenius |
| author_sort | Katherine D. Mosquera |
| collection | DOAJ |
| description | Abstract Background The neotropical anopheline mosquito Anopheles darlingi is a major malaria vector in the Americas. Studies on mosquito-associated microbiota have shown that symbiotic bacteria play a major role in host biology. Mosquitoes acquire and transmit microorganisms over their life cycle. Specifically, the microbiota of immature forms is largely acquired from their aquatic environment. Therefore, our study aimed to describe the microbial communities associated with An. darlingi immature forms and their breeding sites in the Coari municipality, Brazilian Amazon. Methods Larvae, pupae, and breeding water were collected in two different geographical locations. Samples were submitted for DNA extraction and high-throughput 16S rRNA gene sequencing was conducted. Microbial ecology analyses were performed to explore and compare the bacterial profiles of An. darlingi and their aquatic habitats. Results We found lower richness and diversity in An. darlingi microbiota than in water samples, which suggests that larvae are colonized by a subset of the bacterial community present in their breeding sites. Moreover, the bacterial community composition of the immature mosquitoes and their breeding water differed according to their collection sites, i.e., the microbiota associated with An. darlingi reflected that in the aquatic habitats where they developed. The three most abundant bacterial classes across the An. darlingi samples were Betaproteobacteria, Clostridia, and Gammaproteobacteria, while across the water samples they were Gammaproteobacteria, Bacilli, and Alphaproteobacteria. Conclusions Our findings reinforce the current evidence that the environment strongly shapes the composition and diversity of mosquito microbiota. A better understanding of mosquito–microbe interactions will contribute to identifying microbial candidates impacting host fitness and disease transmission. Graphical Abstract |
| first_indexed | 2024-04-09T14:04:17Z |
| format | Article |
| id | doaj.art-546294d8b02d43708435cdbf4bde0bc3 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-09T14:04:17Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-546294d8b02d43708435cdbf4bde0bc32023-05-07T11:07:10ZengBMCParasites & Vectors1756-33052023-05-0116111210.1186/s13071-023-05749-6Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian AmazonKatherine D. Mosquera0Louise K. J. Nilsson1Marta Rodrigues de Oliveira2Elerson Matos Rocha3Osvaldo Marinotti4Sebastian Håkansson5Wanderli P. Tadei6Antonia Queiroz Lima de Souza7Olle Terenius8Department of Cell and Molecular Biology, Biomedical Centre (BMC), Uppsala UniversityDepartment of Cell and Molecular Biology, Biomedical Centre (BMC), Uppsala UniversityPrograma de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE), Universidade do Estado do AmazonasSchool of Agricultural Sciences, Department of Bioprocesses and Biotechnology, Central Multi User Laboratory, São Paulo State University (UNESP)Department of Biology, Indiana UniversityDivision of Applied Microbiology, Department of Chemistry, Lund UniversityLaboratório de Malária e Dengue, Instituto Nacional de Pesquisas da AmazôniaDepartment of Entomology and Acarology, Luiz de Queiroz College of Agriculture (ESALQ), University of São PauloDepartment of Cell and Molecular Biology, Biomedical Centre (BMC), Uppsala UniversityAbstract Background The neotropical anopheline mosquito Anopheles darlingi is a major malaria vector in the Americas. Studies on mosquito-associated microbiota have shown that symbiotic bacteria play a major role in host biology. Mosquitoes acquire and transmit microorganisms over their life cycle. Specifically, the microbiota of immature forms is largely acquired from their aquatic environment. Therefore, our study aimed to describe the microbial communities associated with An. darlingi immature forms and their breeding sites in the Coari municipality, Brazilian Amazon. Methods Larvae, pupae, and breeding water were collected in two different geographical locations. Samples were submitted for DNA extraction and high-throughput 16S rRNA gene sequencing was conducted. Microbial ecology analyses were performed to explore and compare the bacterial profiles of An. darlingi and their aquatic habitats. Results We found lower richness and diversity in An. darlingi microbiota than in water samples, which suggests that larvae are colonized by a subset of the bacterial community present in their breeding sites. Moreover, the bacterial community composition of the immature mosquitoes and their breeding water differed according to their collection sites, i.e., the microbiota associated with An. darlingi reflected that in the aquatic habitats where they developed. The three most abundant bacterial classes across the An. darlingi samples were Betaproteobacteria, Clostridia, and Gammaproteobacteria, while across the water samples they were Gammaproteobacteria, Bacilli, and Alphaproteobacteria. Conclusions Our findings reinforce the current evidence that the environment strongly shapes the composition and diversity of mosquito microbiota. A better understanding of mosquito–microbe interactions will contribute to identifying microbial candidates impacting host fitness and disease transmission. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05749-6Anopheles darlingiMicrobiotaBreeding sitesAmazonMalaria |
| spellingShingle | Katherine D. Mosquera Louise K. J. Nilsson Marta Rodrigues de Oliveira Elerson Matos Rocha Osvaldo Marinotti Sebastian Håkansson Wanderli P. Tadei Antonia Queiroz Lima de Souza Olle Terenius Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon Parasites & Vectors Anopheles darlingi Microbiota Breeding sites Amazon Malaria |
| title | Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon |
| title_full | Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon |
| title_fullStr | Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon |
| title_full_unstemmed | Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon |
| title_short | Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon |
| title_sort | comparative assessment of the bacterial communities associated with anopheles darlingi immature stages and their breeding sites in the brazilian amazon |
| topic | Anopheles darlingi Microbiota Breeding sites Amazon Malaria |
| url | https://doi.org/10.1186/s13071-023-05749-6 |
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