Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections.
Virulence of the most deadly malaria parasite Plasmodium falciparum is linked to the variant surface antigen PfEMP1, which is encoded by about 60 var genes per parasite genome. Although the expression of particular variants has been associated with different clinical outcomes, little is known about...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-04-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1005538 |
| _version_ | 1818582573413040128 |
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| author | Anna Bachmann Michaela Petter Ralf Krumkamp Meral Esen Jana Held Judith A M Scholz Tao Li B Kim Lee Sim Stephen L Hoffman Peter G Kremsner Benjamin Mordmüller Michael F Duffy Egbert Tannich |
| author_facet | Anna Bachmann Michaela Petter Ralf Krumkamp Meral Esen Jana Held Judith A M Scholz Tao Li B Kim Lee Sim Stephen L Hoffman Peter G Kremsner Benjamin Mordmüller Michael F Duffy Egbert Tannich |
| author_sort | Anna Bachmann |
| collection | DOAJ |
| description | Virulence of the most deadly malaria parasite Plasmodium falciparum is linked to the variant surface antigen PfEMP1, which is encoded by about 60 var genes per parasite genome. Although the expression of particular variants has been associated with different clinical outcomes, little is known about var gene expression at the onset of infection. By analyzing controlled human malaria infections via quantitative real-time PCR, we show that parasite populations from 18 volunteers expressed virtually identical transcript patterns that were dominated by the subtelomeric var gene group B and, to a lesser extent, group A. Furthermore, major changes in composition and frequency of var gene transcripts were detected between the parental parasite culture that was used to infect mosquitoes and Plasmodia recovered from infected volunteers, suggesting that P. falciparum resets its var gene expression during mosquito passage and starts with the broad expression of a specific subset of var genes when entering the human blood phase. |
| first_indexed | 2024-12-16T07:51:32Z |
| format | Article |
| id | doaj.art-5464bcb773fb43a8aa635e2277a2665f |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-16T07:51:32Z |
| publishDate | 2016-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-5464bcb773fb43a8aa635e2277a2665f2022-12-21T22:38:51ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-04-01124e100553810.1371/journal.ppat.1005538Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections.Anna BachmannMichaela PetterRalf KrumkampMeral EsenJana HeldJudith A M ScholzTao LiB Kim Lee SimStephen L HoffmanPeter G KremsnerBenjamin MordmüllerMichael F DuffyEgbert TannichVirulence of the most deadly malaria parasite Plasmodium falciparum is linked to the variant surface antigen PfEMP1, which is encoded by about 60 var genes per parasite genome. Although the expression of particular variants has been associated with different clinical outcomes, little is known about var gene expression at the onset of infection. By analyzing controlled human malaria infections via quantitative real-time PCR, we show that parasite populations from 18 volunteers expressed virtually identical transcript patterns that were dominated by the subtelomeric var gene group B and, to a lesser extent, group A. Furthermore, major changes in composition and frequency of var gene transcripts were detected between the parental parasite culture that was used to infect mosquitoes and Plasmodia recovered from infected volunteers, suggesting that P. falciparum resets its var gene expression during mosquito passage and starts with the broad expression of a specific subset of var genes when entering the human blood phase.https://doi.org/10.1371/journal.ppat.1005538 |
| spellingShingle | Anna Bachmann Michaela Petter Ralf Krumkamp Meral Esen Jana Held Judith A M Scholz Tao Li B Kim Lee Sim Stephen L Hoffman Peter G Kremsner Benjamin Mordmüller Michael F Duffy Egbert Tannich Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. PLoS Pathogens |
| title | Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. |
| title_full | Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. |
| title_fullStr | Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. |
| title_full_unstemmed | Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. |
| title_short | Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections. |
| title_sort | mosquito passage dramatically changes var gene expression in controlled human plasmodium falciparum infections |
| url | https://doi.org/10.1371/journal.ppat.1005538 |
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