Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation
Information on the basic changes associated with green tea small molecules in acute inflammation is deficient. The purpose of the study was to characterize and establish the effects of green tea silver nanoparticles (AgNPs) following inflammation in BALB/c male mice. In this study, green tea silver...
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| Language: | English |
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Elsevier
2023-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023011295 |
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| author | Herbert Izo Ninsiima Ejike Daniel Eze Kenneth Ssekatawa Halima Nalugo Caroline Asekenye David Onanyang Edson Ireeta Munanura Moses Ariong Kevin Matama Gerald Zirintunda Ngala Elvis Mbiydzenyuy Fred Ssempijja Adam Moyosore Afodun Regan Mujinya Ibe Michael Usman Oscar Hilary Asiimwe Julius Tibyangye Keneth Iceland Kasozi |
| author_facet | Herbert Izo Ninsiima Ejike Daniel Eze Kenneth Ssekatawa Halima Nalugo Caroline Asekenye David Onanyang Edson Ireeta Munanura Moses Ariong Kevin Matama Gerald Zirintunda Ngala Elvis Mbiydzenyuy Fred Ssempijja Adam Moyosore Afodun Regan Mujinya Ibe Michael Usman Oscar Hilary Asiimwe Julius Tibyangye Keneth Iceland Kasozi |
| author_sort | Herbert Izo Ninsiima |
| collection | DOAJ |
| description | Information on the basic changes associated with green tea small molecules in acute inflammation is deficient. The purpose of the study was to characterize and establish the effects of green tea silver nanoparticles (AgNPs) following inflammation in BALB/c male mice. In this study, green tea silver nitrate nanoparticles were characterized and the extract were made up to constitute high (100%), medium (10%), and low (1%) concentrations for administration. Acute inflammation was induced in groups I–V of the experimental rodents by injecting 0.5 ml/kg of fresh egg albumin on the subplantar surface of the right hind paw and animals were monitored for 36 h. Group I–III were administered 100%, 10%, 1% green tea nanoparticles extract while group IV was given diclofenac. Group V was the positive control while group VI was the negative control that received the vehicle. Paw edema was measured at a 2 h interval for 3 days, while the pain was assessed by measuring the locomotion activity using the voluntary wheel running and the anxiety-like behavior. Hypersensitivity was measured through the temperature sensation experiment and a non-linear regression analysis was done. Here, synthesized green tea AgNPs registered an absorbance band at 460 nm, phytochemicals due to presence of organic functional groups of OCO of oxycarbons, of CC of a conjugate alkene, CO of a stretching bond of a secondary alcohol. The silver green tea nanoparticles were spherical, covered by a slimy layer, capped and stable. Green tea AgNPs significantly decreased temperature hypersensitivity in BALB/c male mice and this demonstrated their protective effects. Low concentrations of green tea nanoparticles inhibited edema thus mimicking effects of diclofenac, however, the percentage of inhibition was highest in medium and high silver-tea nanoparticles concentrations demonstration the importance of concentration in therapeutics. Anxiety was lowest in BALB/c male mice treated with high concentrations of silver green tea nanoparticles, and this led to increased locomotory activity in mice. Green tea AgNPs have strong anti-inflammatory effects at high concentrations. Concentrations of green tea AgNPs modulated basic sensory and motor behaviors in BALB/c male mice demonstrating their importance in complementary and integrative medical practice. |
| first_indexed | 2024-04-09T19:24:24Z |
| format | Article |
| id | doaj.art-5465ab269a9e44539bfc8eee838effde |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:24:24Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5465ab269a9e44539bfc8eee838effde2023-04-05T08:19:18ZengElsevierHeliyon2405-84402023-03-0193e13922Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammationHerbert Izo Ninsiima0Ejike Daniel Eze1Kenneth Ssekatawa2Halima Nalugo3Caroline Asekenye4David Onanyang5Edson Ireeta Munanura6Moses Ariong7Kevin Matama8Gerald Zirintunda9Ngala Elvis Mbiydzenyuy10Fred Ssempijja11Adam Moyosore Afodun12Regan Mujinya13Ibe Michael Usman14Oscar Hilary Asiimwe15Julius Tibyangye16Keneth Iceland Kasozi17Department of Physiology, School of Medicine, Kabale University, Box 317, Kabale, UgandaDepartment of Physiology, School of Medicine, Kabale University, Box 317, Kabale, UgandaDepartment of Veterinary Pharmacy, Clinics and Comparative Medicine, School of Veterinary Medicine and Animal Resources, Makerere University, Box 7062, Kampala, UgandaDepartment of Anatomy, Faculty of Medicine, Mbarara University of Science and Technology, Box 1410 Mbarara, UgandaDepartment of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Kampala International University Western Campus, Box 71, Bushenyi, UgandaDepartment of Biology, Faculty of Science, Gulu University, P.O. Box 166, Gulu, UgandaDepartment of Pharmacy, College of Health Sciences, Makerere University, Box 7062, Kampala, UgandaDepartment of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Kampala International University Western Campus, Box 71, Bushenyi, UgandaDepartment of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Kampala International University Western Campus, Box 71, Bushenyi, UgandaDepartment of Animal Production and Management, Faculty of Agriculture and Animal Sciences, Busitema University, Box 206, Soroti, UgandaDepartment of Basic Medical Sciences, Michael Chilufya Sata School of Medicine, Copperbelt University, Ndola, ZambiaDepartment of Anatomy, Faculty of Medicine, Mbarara University of Science and Technology, Box 1410 Mbarara, UgandaDepartment of Anatomy and Cell Biology, Faculty of Health Sciences, Busitema University, UgandaFaculty of Biomedical Sciences, Kampala International University Western Campus, Box 71, Bushenyi, UgandaFaculty of Biomedical Sciences, Kampala International University Western Campus, Box 71, Bushenyi, UgandaSchool of Health Sciences, Mountains of the Moon University, Fort Portal, UgandaMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen, AB243UE, Scotland, United Kingdom; Department of Medical Laboratory Sciences, Faculty of Health Sciences, Muni University, P.O Box 725, Arua, UgandaDepartment of Physiology, School of Medicine, Kabale University, Box 317, Kabale, Uganda; Infection Medicine, Deanery of Biomedical Sciences, College of Medicine and Veterinary Medicine, University of Edinburgh, EH8 9JZ, Edinburgh, United Kingdom; Corresponding author. Department of Physiology, School of Medicine, Kabale University, Box 317, Kabale, Uganda.Information on the basic changes associated with green tea small molecules in acute inflammation is deficient. The purpose of the study was to characterize and establish the effects of green tea silver nanoparticles (AgNPs) following inflammation in BALB/c male mice. In this study, green tea silver nitrate nanoparticles were characterized and the extract were made up to constitute high (100%), medium (10%), and low (1%) concentrations for administration. Acute inflammation was induced in groups I–V of the experimental rodents by injecting 0.5 ml/kg of fresh egg albumin on the subplantar surface of the right hind paw and animals were monitored for 36 h. Group I–III were administered 100%, 10%, 1% green tea nanoparticles extract while group IV was given diclofenac. Group V was the positive control while group VI was the negative control that received the vehicle. Paw edema was measured at a 2 h interval for 3 days, while the pain was assessed by measuring the locomotion activity using the voluntary wheel running and the anxiety-like behavior. Hypersensitivity was measured through the temperature sensation experiment and a non-linear regression analysis was done. Here, synthesized green tea AgNPs registered an absorbance band at 460 nm, phytochemicals due to presence of organic functional groups of OCO of oxycarbons, of CC of a conjugate alkene, CO of a stretching bond of a secondary alcohol. The silver green tea nanoparticles were spherical, covered by a slimy layer, capped and stable. Green tea AgNPs significantly decreased temperature hypersensitivity in BALB/c male mice and this demonstrated their protective effects. Low concentrations of green tea nanoparticles inhibited edema thus mimicking effects of diclofenac, however, the percentage of inhibition was highest in medium and high silver-tea nanoparticles concentrations demonstration the importance of concentration in therapeutics. Anxiety was lowest in BALB/c male mice treated with high concentrations of silver green tea nanoparticles, and this led to increased locomotory activity in mice. Green tea AgNPs have strong anti-inflammatory effects at high concentrations. Concentrations of green tea AgNPs modulated basic sensory and motor behaviors in BALB/c male mice demonstrating their importance in complementary and integrative medical practice.http://www.sciencedirect.com/science/article/pii/S2405844023011295Small moleculesUganda teaThe safety of teaAgNPsPhysiology of nanoparticlesGreen tea synthesis |
| spellingShingle | Herbert Izo Ninsiima Ejike Daniel Eze Kenneth Ssekatawa Halima Nalugo Caroline Asekenye David Onanyang Edson Ireeta Munanura Moses Ariong Kevin Matama Gerald Zirintunda Ngala Elvis Mbiydzenyuy Fred Ssempijja Adam Moyosore Afodun Regan Mujinya Ibe Michael Usman Oscar Hilary Asiimwe Julius Tibyangye Keneth Iceland Kasozi Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation Heliyon Small molecules Uganda tea The safety of tea AgNPs Physiology of nanoparticles Green tea synthesis |
| title | Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation |
| title_full | Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation |
| title_fullStr | Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation |
| title_full_unstemmed | Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation |
| title_short | Green tea silver nanoparticles improve physiological motor and cognitive function in BALB/c mice during inflammation |
| title_sort | green tea silver nanoparticles improve physiological motor and cognitive function in balb c mice during inflammation |
| topic | Small molecules Uganda tea The safety of tea AgNPs Physiology of nanoparticles Green tea synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023011295 |
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